69 research outputs found
Conformational Altered p53 as an Early Marker of Oxidative Stress in Alzheimer's Disease
In order to study oxidative stress in peripheral cells of Alzheimer's disease (AD) patients, immortalized lymphocytes derived from two peculiar cohorts of patients, referring to early onset AD (EOSAD) and subjects harboured AD related mutation (ADmut), were used. Oxidative stress was evaluated measuring i) the typical oxidative markers, such as HNE Michel adducts, 3 Nitro-Tyrosine residues and protein carbonyl on protein extracts, ii) and the antioxidant capacity, following the enzymatic kinetic of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRD). We found that the signs of oxidative stress, measured as oxidative marker levels, were evident only in ADmut but not in EOSAD patients. However, oxidative imbalance in EOSAD as well as ADmut lymphocytes was underlined by a reduced SOD activity and GRD activity in both pathological groups in comparison with cells derived from healthy subjects. Furthermore, a redox modulated p53 protein was found conformational altered in both EOSAD and ADmut B lymphocytes in comparison with control cells. This conformational altered p53 isoform, named “unfolded p53”, was recognized by the use of two specific conformational anti-p53 antibodies. Immunoprecipitation experiments, performed with the monoclonal antibodies PAb1620 (that recognizes p53wt) and PAb240 (that is direct towards unfolded p53), and followed by the immunoblotting with anti-4-hydroxynonenal (HNE) and anti- 3-nitrotyrosine (3NT) antibodies, showed a preferential increase of nitrated tyrosine residues in unfolded p53 isoform comparing to p53 wt protein, in both ADmut and EOSAD. In addition, a correlation between unfolded p53 and SOD activity was further found. Thus this study suggests that ROS/RNS contributed to change of p53 tertiary structure and that unfolded p53 can be considered as an early marker of oxidative imbalance in these patients
Hybridization and adaptive evolution of diverse Saccharomyces species for cellulosic biofuel production
Additional file 15. Summary of whole genome sequencing statistics
The effect of altosid, a preparation with hormone-like activity, on the metamorphosis of house fly Musca demestica L.
Differentiation of sensitivity of Pediculus humanus humanus L. to the organochlorine insecticides in relation to physiological and enviromental conditions. Part III. Effects of enviromental conditions (temperature, humidity) and of propagation conditions (concentration of the culture) on the sensitivity of body louse to p,p'-DDT and gamma-HCH
Chuvstvitel'nost' platjanykh vshejj Pediculus humanus humanus L. k khlorirovannym insekticidam v zavisimosti ot temperatury
pp' DDT izomer and Lindan (hexachlorocyclohexane) were used; the sensitivity to pp' DDT showed no appreciable changes in the temperature range of 25-30°, its increase being observed at 37°C. The sensitivity to the fumigating action of gamma HCH izomer is maximal at 37° and minimal at 25°C
Differentation of sensitivity of Pediculus humanus humanus L. on chloroganic insecticides in relation to physiological and environmental conditions. Part II. Influence of physiological conditions such as age, satiety on sensitivity of pediculus humanus on p.p'DDT and gamma HCH
The action of permetrine and borax in poisoned food on certain insects of hygienic importance
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