Apparatus enables automatic microanalysis of body fluids

Apparatus will automatically and quantitatively determine body fluid constituents which are amenable to analysis by fluorometry or colorimetry. The results of the tests are displayed as percentages of full scale deflection on a strip-chart recorder. The apparatus can also be adapted for microanalysis of various other fluids

Facile syntheses of building blocks for the construction of phosphotyrosine mimetics

The copper-catalysed zinc phosphonate chemistry described by Yokomatsu and Shibuya can be used to enter the classical organometallic coupling repertoire via Stille and Suzuki–Miyaura couplings. 1,4-Diiodobenzene underwent coupling with the organozinc reagent derived from diethyl bromodifluoromethylphosphonate with copper(I) catalysis to afford diethyl (4-iodophenyl)difluoromethylphosphonate. Higher yielding couplings were run with (4-trifluoromethylsulfonyloxy)- and (4-nonafluorobutylsulfonyloxy)-iodobenzenes. The iodide and the triflate coupled under palladium-catalysed conditions with a range of stannanes and boronic acids in moderate to excellent yields. Shibuya–Yokomatsu couplings were also successful with more functionalised iodoarenes and heteroarenes presenting the important phosphate mimic on a range of scaffolds

Controlled Nanoparticle Formation by Diffusion Limited Coalescence

Polymeric nanoparticles (NPs) have a great application potential in science and technology. Their functionality strongly depends on their size. We present a theory for the size of NPs formed by precipitation of polymers into a bad solvent in the presence of a stabilizing surfactant. The analytical theory is based upon diffusion-limited coalescence kinetics of the polymers. Two relevant time scales, a mixing and a coalescence time, are identified and their ratio is shown to determine the final NP diameter. The size is found to scale in a universal manner and is predominantly sensitive to the mixing time and the polymer concentration if the surfactant concentration is sufficiently high. The model predictions are in good agreement with experimental data. Hence the theory provides a solid framework for tailoring nanoparticles with a priori determined size.Comment: 4 pages, 3 figure

Astrometric Discovery of GJ 164B

We discovered a low-mass companion to the M-dwarf GJ 164 with the CCD-based imaging system of the Stellar Planet Survey (STEPS) astrometric program. The existence of GJ 164B was confirmed with Hubble Space Telescope NICMOS imaging observations. A high-dispersion spectral observation in V sets a lower limit of delta m> 2.2 mag between the two components of the system. Based upon our parallax value of 0.082 +/- 0.008, we derive the following orbital parameters: P = 2.04 +/- 0.03 y, a = 1.03 +/- 0.03 AU, and Mtotal = 0.265 +/- 0.020 MSun. The component masses are MA = 0.170 +/- 0.015 MSun and MB = 0.095 +/- 0.015 MSun. Based on its mass, colors, and spectral properties, GJ 164B has spectral type M6-8 V.Comment: pdf file 14 pages with 6 fig

In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons

In vivo whole-cell recording when combined with morphological characterization after biocytin labeling is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we provide a step-by-step procedure for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. Two types of visual stimulation methods are described. While we focus on superior colliculus neurons, this protocol is applicable to other brain areas.Comment: 29 pages including 4 figure

Prospects for Redshifted 21-cm observations of quasar HII regions

The introduction of low-frequency radio arrays over the coming decade is expected to revolutionize the study of the reionization epoch. Observation of the contrast in redshifted 21cm emission between a large HII region and the surrounding neutral IGM will be the simplest and most easily interpreted signature. We find that an instrument like the planned Mileura Widefield Array Low-Frequency Demonstrator (LFD) will be able to obtain good signal to noise on HII regions around the most luminous quasars, and determine some gross geometric properties, e.g. whether the HII region is spherical or conical. A hypothetical follow-up instrument with 10 times the collecting area of the LFD (MWA-5000) will be capable of mapping the detailed geometry of HII regions, while SKA will be capable of detecting very narrow spectral features as well as the sharpness of the HII region boundary. The MWA-5000 will discover serendipitous HII regions in widefield observations. We estimate the number of HII regions which are expected to be generated by quasars. Assuming a late reionization at z~6 we find that there should be several tens of quasar HII regions larger than 4Mpc at z~6-8 per field of view. Identification of HII regions in forthcoming 21cm surveys can guide a search for bright galaxies in the middle of these regions. Most of the discovered galaxies would be the massive hosts of dormant quasars that left behind fossil HII cavities that persisted long after the quasar emission ended, owing to the long recombination time of intergalactic hydrogen. A snap-shot survey of candidate HII regions selected in redshifted 21cm image cubes may prove to be the most efficient method for finding very high redshift quasars and galaxies.Comment: 14 pages, 8 figures. Submitted to Ap

Improved Moving Puncture Gauge Conditions for Compact Binary Evolutions

Robust gauge conditions are critically important to the stability and accuracy of numerical relativity (NR) simulations involving compact objects. Most of the NR community use the highly robust---though decade-old---moving-puncture (MP) gauge conditions for such simulations. It has been argued that in binary black hole (BBH) evolutions adopting this gauge, noise generated near adaptive-mesh-refinement (AMR) boundaries does not converge away cleanly with increasing resolution, severely limiting gravitational waveform accuracy at computationally feasible resolutions. We link this noise to a sharp (short-wavelength), initial outgoing gauge wave crossing into progressively lower resolution AMR grids, and present improvements to the standard MP gauge conditions that focus on stretching, smoothing, and more rapidly settling this outgoing wave. Our best gauge choice greatly reduces gravitational waveform noise during inspiral, yielding less fluctuation in convergence order and $\sim 40$ lower waveform phase and amplitude errors at typical resolutions. Noise in other physical quantities of interest is also reduced, and constraint violations drop by more than an order of magnitude. We expect these improvements will carry over to simulations of all types of compact binary systems, as well as other $N$+1 formulations of gravity for which MP-like gauge conditions can be chosen.Comment: 25 pages, 16 figures, 2 tables. Matches published versio

An all-optical buffer based on temporal cavity solitons operating at 10 Gb/s

We demonstrate the operation of an all-optical buffer based on temporal cavity solitons stored in a nonlinear passive fiber ring resonator. Unwanted acoustic interactions between neighboring solitons are suppressed by modulating the phase of the external laser driving the cavity. A new locking scheme is presented that allows the buffer to operate with an arbitrarily large number of cavity solitons in the loop. Experimentally, we are able to demonstrate the storage of 4536 bits of data, written all-optically into the fiber ring at 10 Gb/s, for 1 minute.Comment: 5 pages, 4 figure

Acoustic characterization of crack damage evolution in sandstone deformed under conventional and true triaxial loading

We thank the Associate Editor, Michelle Cooke, and the reviewers, Ze'ev Reches and Yves Guéguen, for useful comments which helped to improve the manuscript. We thank J.G. Van Munster for providing access to the true triaxial apparatus at KSEPL and for technical support during the experimental program. We thank R. Pricci for assistance with technical drawings of the apparatus. This work was partly funded by NERC award NE/N002938/1 and by a NERC Doctoral Studentship, which we gratefully acknowledge. Supporting data are included in a supporting information file; any additional data may be obtained from J.B. (e-mail: [email protected]).Peer reviewedPublisher PD