17 research outputs found
Selected Preconception Health Indicators and Birth Weight Disparities in a National Study
Get PDFThis analysis explored the effect of timing, sequencing, and change in preconception health across adolescence and young adulthood on racial/ethnic disparities in birth weight in a diverse national cohort of young adult women
Origins of Disparities in Cardiovascular Disease: Birth Weight, Body Mass Index, and Young Adult Systolic Blood Pressure in the National Longitudinal Study of Adolescent Health
Get PDFWe evaluated the contributions of birth weight and current body mass index (BMI) to racial/ethnic disparities in systolic blood pressure (SBP) in the U.S
Preconception Health Trajectories and Birth Weight in a National Prospective Cohort
Get PDFThis study was designed to assess the relationship between birth weight and prospectively measured trajectories of preconception health across adolescence and young adulthood in a diverse national cohort of young adult women
Recording temporal data with minutes resolution into DNA
Get PDFRecording complex biological signals is a crucial application of synthetic biology and essential for a deeper understanding of biological processes. An ideal ābiorecorderā would have the ability to record biological signals over a wide spatial distribution of cells with high temporal resolution. However, the genetically encoded biorecording tools available have very good spatial resolution (cellular level), but currently rely on turning on and off transcription and translation of a protein (e.g., Cas9 or a recombinase) to record the biological signal, making their temporal resolution on the order of hours. Here we introduce a DNA polymerase based biorecorder that can record cationic concentration fluctuations into DNA sequence with a resolution of ~1 minute. We use a template independent DNA polymerase; terminal deoxynucleotidyl transferase (TdT) that randomly incorporates bases onto a single strand of DNA. The preference of base incorporated by TdT changes with the concentration of cations in TdTās environment. Therefore, by analyzing a strand of DNA that was extended in fluctuating cation concentrations, we can determine the temporal profile of cation concentration from the bases added. Using this method, we can measure a change in Co2+ concentration during a one hour period with an accuracy of 1 min. We also show the approach works for Zn2+ and Ca2+. We will present our methods for optimizing this biorecorder and characterize its performance in vitro. Recording data onto DNA with minutes time resolution could solve many challenging data acquisition problems in neuroscience and developmental biology, and could aid in the use of DNA as a data storage medium
