Analysis of the topography of microchannels with different sizes

This paper was presented at the 2nd Micro and Nano Flows Conference (MNF2009), which was held at Brunel University, West London, UK. The conference was organised by Brunel University and supported by the Institution of Mechanical Engineers, IPEM, the Italian Union of Thermofluid dynamics, the Process Intensification Network, HEXAG - the Heat Exchange Action Group and the Institute of Mathematics and its Applications.The longterm aim in the design of modern evaporators is to find a heat transfer calculation method based on the phenomena of growing and departing vapour bubbles on the heated surface. New developments in computer calculation and in measurement technique support the local analysis of the heat transfer, bubble formation and the microstructure of the heated surface. All three kinds of data originate from the same equipment and from experiments performed with equal detail and precision. The influence of different rough and structured surfaces on the heat transfer coefficient will be investigated by a joint research project of Brunel and Hannover Universities with refrigerants boiling in single microchannels of different inner diameter. The microchannels are capillary tubes and they are manufactured by industrial processes. The samples of the microchannels have to be prepared by special methods, because the surfaces inside the microchannels cannot be measured in the same way as used for the heat transfer measurements. The microstructure of the surfaces of three microchannels (D = 0.4, 0.5 and 0.8mm) is measured by a contact stylus instrument. The paper will focus on the detailed description of the profiles and the topographies of the samples. The roughness parameter in accordance with standard practice are calculated for ca 1000 runs (profiles). The roughness parameters of the smaller microchannels are twofold higher than those of the largest microchannel investigated. The scattering of the roughness of the largest microchannel (D = 0.8 mm) is less and the cavities are smaller than those of the other ones (D = 0.4 and 0.5 mm). All surfaces investigated demonstrate a deterministic microstructure as for emery ground surfaces on smooth tubes in the literature

Retinoic Acid Functions as a Key GABAergic Differentiation Signal in the Basal Ganglia

Retinoic acid (RA) is essential for the generation of GABAergic inhibitory neurons in the mouse forebrain, and RA treatment of embryonic stem cells induces the production of GABAergic neurons

Transient receptor potential canonical 4 and 5 proteins as targets in cancer therapeutics

Novel approaches towards cancer therapy are urgently needed. One approach might be to target ion channels mediating Ca²+ entry because of the critical roles played by Ca²+ in many cell types, including cancer cells. There are several types of these ion channels, but here we address those formed by assembly of transient receptor potential canonical (TRPC) proteins, particularly those which involve two closely related members of the family: TRPC4 and TRPC5. We focus on these proteins because recent studies point to roles in important aspects of cancer: drug resistance, transmission of drug resistance through extracellular vesicles, tumour vascularisation, and evoked cancer cell death by the TRPC4/5 channel activator (−)-englerin A. We conclude that further research is both justified and necessary before these proteins can be considered as strong targets for anti-cancer cell drug discovery programmes. It is nevertheless already apparent that inhibitors of the channels would be unlikely to cause significant adverse effects, but, rather, have other effects which may be beneficial in the context of cancer and chemotherapy, potentially including suppression of innate fear, visceral pain and pathological cardiac remodelling

Present state and future perspectives of using pluripotent stem cells in toxicology research

The use of novel drugs and chemicals requires reliable data on their potential toxic effects on humans. Current test systems are mainly based on animals or in vitro–cultured animal-derived cells and do not or not sufficiently mirror the situation in humans. Therefore, in vitro models based on human pluripotent stem cells (hPSCs) have become an attractive alternative. The article summarizes the characteristics of pluripotent stem cells, including embryonic carcinoma and embryonic germ cells, and discusses the potential of pluripotent stem cells for safety pharmacology and toxicology. Special attention is directed to the potential application of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) for the assessment of developmental toxicology as well as cardio- and hepatotoxicology. With respect to embryotoxicology, recent achievements of the embryonic stem cell test (EST) are described and current limitations as well as prospects of embryotoxicity studies using pluripotent stem cells are discussed. Furthermore, recent efforts to establish hPSC-based cell models for testing cardio- and hepatotoxicity are presented. In this context, methods for differentiation and selection of cardiac and hepatic cells from hPSCs are summarized, requirements and implications with respect to the use of these cells in safety pharmacology and toxicology are presented, and future challenges and perspectives of using hPSCs are discussed