1,924 research outputs found
Identification of individual enzymes in crude extracts by acrylamide gel electrophoresis
Identification of enzymes by acrylamide gel electrophoresi
Scalable iterative methods for sampling from massive Gaussian random vectors
Sampling from Gaussian Markov random fields (GMRFs), that is multivariate
Gaussian ran- dom vectors that are parameterised by the inverse of their
covariance matrix, is a fundamental problem in computational statistics. In
this paper, we show how we can exploit arbitrarily accu- rate approximations to
a GMRF to speed up Krylov subspace sampling methods. We also show that these
methods can be used when computing the normalising constant of a large
multivariate Gaussian distribution, which is needed for both any
likelihood-based inference method. The method we derive is also applicable to
other structured Gaussian random vectors and, in particu- lar, we show that
when the precision matrix is a perturbation of a (block) circulant matrix, it
is still possible to derive O(n log n) sampling schemes.Comment: 17 Pages, 4 Figure
Rehydrating ascospores to improve germination
Rehydrating ascospores to improve germinatio
Feedback in the local LBG Analog Haro 11 as probed by far-UV and X-ray observations
We have re-analyzed FUSE data and obtained new Chandra observations of Haro
11, a local (D_L=88 Mpc) UV luminous galaxy. Haro 11 has a similar far-UV
luminosity (10^10.3 L_\odot), UV surface brightness (10^9.4 L_\odot kpc^-2),
SFR, and metallicity to that observed in Lyman Break Galaxies (LBGs). We show
that Haro 11 has extended, soft thermal (kT~0.68 keV) X-ray emission with a
luminosity and size which scales with the physical properties (e.g. SFR,
stellar mass) of the host galaxy. An enhanced alpha/Fe, ratio of ~4 relative to
solar abundance suggests significant supernovae enrichment. These results are
consistent with the X-ray emission being produced in a shock between a
supernovae driven outflow and the ambient material. The FUV spectra show strong
absorption lines similar to those observed in LBG spectra. A blueshifted
absorption component is identified as a wind outflowing at ~200-280 km/s.
OVI\lambda\lambda1032,1038 emission, the dominant cooling mechanism for coronal
gas at T~10^5.5 K is also observed. If associated with the outflow, the
luminosity of the OVI emission suggests that <20% of the total mechanical
energy from the supernovae and solar winds is being radiated away. This implies
that radiative cooling through OVI is not significantly inhibiting the growth
of the outflowing gas. In contradiction to the findings of Bergvall et al 2006,
we find no convincing evidence of Lyman continuum leakage in Haro 11. We
conclude that the wind has not created a `tunnel' allowing the escape of a
significant fraction of Lyman continuum photons and place a limit on the escape
fraction of f_{esc}<2%. Overall, both Haro 11 and a previously observed LBG
analogue VV 114, provide an invaluable insight into the X-ray and FUV
properties of high redshift LBGs.Comment: Accepted for publication in ApJ, 40 pages, 17 figure
Ferromagnetic redshift of the optical gap in GdN
We report measurements of the optical gap in a GdN film at temperatures from
300 to 6K, covering both the paramagnetic and ferromagnetic phases. The gap is
1.31eV in the paramagnetic phase and red-shifts to 0.9eV in the spin-split
bands below the Curie temperature. The paramagnetic gap is larger than was
suggested by very early experiments, and has permitted us to refine a
(LSDA+U)-computed band structure. The band structure was computed in the full
translation symmetry of the ferromagnetic ground state, assigning the
paramagnetic-state gap as the average of the majority- and minority-spin gaps
in the ferromagnetic state. That procedure has been further tested by a band
structure in a 32-atom supercell with randomly-oriented spins. After fitting
only the paramagnetic gap the refined band structure then reproduces our
measured gaps in both phases by direct transitions at the X point.Comment: 5 pages, 4 figure
Development of targeted messages to promote smoking cessation among construction trade workers
Blue-collar workers, particularly those in the construction trades, are more likely to smoke and have less success in quitting when compared with white-collar workers. Little is known about health communication strategies that might influence this priority population. This article describes our formative work to develop targeted messages to increase participation in an existing smoking cessation program among construction workers. Using an iterative and sequential mixed-methods approach, we explored the culture, health attitudes and smoking behaviors of unionized construction workers. We used focus group and survey data to inform message development, and applied audience segmentation methods to identify potential subgroups. Among 144 current smokers, 65% reported wanting to quit smoking in the next 6 months and only 15% had heard of a union-sponsored smoking cessation program, despite widespread advertising. We tested 12 message concepts and 26 images with the target audience to evaluate perceived relevance and effectiveness. Participants responded most favorably to messages and images that emphasized family and work, although responses varied by audience segments based on age and parental status. This study is an important step towards integrating the culture of a high-risk group into targeted messages to increase participation in smoking cessation activities
Molecular Gas in M82: Resolving the Outflow and Streamers
We present a high-resolution (3.6'', 70pc) CO(1-0) mosaic of the molecular
gas in M 82 covering an area of 2.5' x 3.5' (2.8kpc x 3.9kpc) obtained with the
OVRO millimeter interferometer. The observations reveal the presence of huge
amounts of molecular gas (> 70% of the total molecular mass, M_tot=1.3 x 10^9
M_sun) outside the central 1 kpc disk. Molecular streamers are detected in and
below M82's disk out to distances from the center of 1.7 kpc. Some of these
streamers are well correlated with optical absorption features; they form the
basis of some of the prominent tidal HI features around M 82. This provides
evidence that the molecular gas within M 82's optical disk is disrupted by the
interaction with M 81. Molecular gas is found in M 82's outflow/halo, reaching
distances up to 1.2 kpc below the plane; CO line-splitting has been detected
for the first time in the outflow. The maximum outflow velocity is 230 km/s; we
derive an opening angle of 55 deg for the molecular outflow cone. The total
amount of gas in the outflow is >3 x 10^8 M_sun and its kinetic energy is of
order 10^55 erg, about one percent of the estimated total mechanical energy
input of M 82's starburst. Our study implies that extreme starburst
environments can move significant amounts of molecular gas in to a galaxy's
halo (and even to the intergalactic medium).Comment: accepted for publication in the ApJ Letters full PS file @
http://www.aoc.nrao.edu/~fwalter/walter_m82.p
Spatially Resolved Spitzer-IRS Spectroscopy of the Central Region of M82
We present high spatial resolution (~ 35 parsec) 5-38 um spectra of the
central region of M82, taken with the Spitzer Infrared Spectrograph. From these
spectra we determined the fluxes and equivalent widths of key diagnostic
features, such as the [NeII]12.8um, [NeIII]15.5um, and H_2 S(1)17.03um lines,
and the broad mid-IR polycyclic aromatic hydrocarbon (PAH) emission features in
six representative regions and analysed the spatial distribution of these lines
and their ratios across the central region. We find a good correlation of the
dust extinction with the CO 1-0 emission. The PAH emission follows closely the
ionization structure along the galactic disk. The observed variations of the
diagnostic PAH ratios across M82 can be explained by extinction effects, within
systematic uncertainties. The 16-18um PAH complex is very prominent, and its
equivalent width is enhanced outwards from the galactic plane. We interpret
this as a consequence of the variation of the UV radiation field. The EWs of
the 11.3um PAH feature and the H_2 S(1) line correlate closely, and we conclude
that shocks in the outflow regions have no measurable influence on the H_2
emission. The [NeIII]/[NeII] ratio is on average low at ~0.18, and shows little
variations across the plane, indicating that the dominant stellar population is
evolved (5 - 6 Myr) and well distributed. There is a slight increase of the
ratio with distance from the galactic plane of M82 which we attribute to a
decrease in gas density. Our observations indicate that the star formation rate
has decreased significantly in the last 5 Myr. The quantities of dust and
molecular gas in the central area of the galaxy argue against starvation and
for negative feedback processes, observable through the strong extra-planar
outflows.Comment: 15 pages, 12 figures, 3 tables, ApJ, emulateap
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