Exploring sustainability potentials in vineyards through LCA? Evidence from farming practices in South Africa

Following the urgency to curb environmental impacts across all sectors globally, this is the first life cycle assessment of different wine grape farming practices suitable for commercial conventional production in South Africa, aiming at better understanding the potentials to reduce adverse effects on the environment and on human health.Purpose Following the urgency to curb environmental impacts across all sectors globally, this is the first life cycle assessment of different wine grape farming practices suitable for commercial conventional production in South Africa, aiming at better understanding the potentials to reduce adverse effects on the environment and on human health. Methods An attributional life cycle assessment was conducted on eight different scenarios that reduce the inputs of herbicides and insecticides compared against a business as usual (BAU) scenario. We assess several impact categories based on ReCiPe, namely global warming potential, terrestrial acidification, freshwater eutrophication, terrestrial toxicity, freshwater toxicity, marine toxicity, human carcinogenic toxicity and human non-carcinogenic toxicity, human health and ecosystems. A water footprint assessment based on the AWARE method accounts for potential impacts within the watershed. Results and discussion Results show that in our impact assessment, more sustainable farming practices do not always outperform the BAU scenario, which relies on synthetic fertiliser and agrochemicals. As a main trend, most of the impact categories were dominated by energy requirements of wine grape production in an irrigated vineyard, namely the usage of electricity for irrigation pumps and diesel for agricultural machinery. The most favourable scenario across the impact categories provided a low diesel usage, strongly reduced herbicides and the absence of insecticides as it applied cover crops and an integrated pest management. Pesticides and heavy metals contained in agrochemicals are the main contributors to emissions to soil that affected the toxicity categories and impose a risk on human health, which is particularly relevant for the manual labour-intensive South African wine sector. However, we suggest that impacts of agrochemicals on human health and the environment are undervalued in the assessment. The 70% reduction of toxic agrochemicals such as Glyphosate and Paraquat and the 100% reduction of Chlorpyriphos in vineyards hardly affected the model results for human and ecotoxicity. Our concerns are magnified by the fact that manual labour plays a substantial role in South African vineyards, increasing the exposure of humans to these toxic chemicals at their workplace. Conclusions A more sustainable wine grape production is possible when shifting to integrated grape production practices that reduce the inputs of agrochemicals. Further, improved water and related electricity management through drip irrigation, deficit irrigation and photovoltaic-powered irrigation is recommendable, relieving stress on local water bodies, enhancing drought-preparedness planning and curbing CO2 emissions embodied in products.Peer reviewe

Vine Signal Extraction - an Application of remote sensing in precision Viticulture

This paper presents a study of precision agriculture in the wine industry. While precision viticulture mostly aims to maximise yields by delivering the right inputs to appropriate places on a farm in the correct doses and at the right time, the objective of this study was rather to assess vine biomass differences. The solution proposed in this paper uses aerial imagery as the primary source of data for vine analysis. The first objective to be achieved by the solution is to automatically identify vineyards blocks, vine rows, and individual vines within rows. This is made possible through a series of enhancements and hierarchical segmentations of the aerial images. The second objective is to determine the correlation of image data with the biophysical data (yield and pruning mass) of each vine. A multispectral aerial image is used to compute vegetation indices, which serve as indicators of biophysical measures. The results of the automatic detection are compared against a test field, to verify both vine location and vegetation index correlation with relevant vine parameters. The advantage of this technique is that it functions in environments where active cover crop growth between vines is evident and where variable vine canopy conditions are present within a vineyard block

GRA.LE.D. (GRApevine LEaf Digitalization) Software for the Detection and Graphic Reconstruction of Ampelometric Differences Between Vitis Leaves

Raster graphic ampelometric software was not exclusively developed for the estimation of leaf area,but also for the characterization of grapevine (Viti vinifera L.) leaves. The software was written in C++programming language, using the C++ Builder 2007 for Windows 95-XP and Linux operation systems. Ithandles desktop-scanned images. On the image analysed with the GRA.LE.D., the user has to determine11 points. These points are then connected and the distances between them calculated. The GRA.LE.D.software supports standard ampelometric measurements such as leaf area, angles between the veins andlengths of the veins. These measurements are recorded by the software and exported into plain ASCII textfiles for single or multiple samples. Twenty-two biometric data points of each leaf are identified by theGRA.LE.D. It presents the opportunity to statistically analyse experimental data, allows comparison ofcultivars and enables graphic reconstruction of leaves using the Microsoft Excel Chart Wizard. The GRA.LE.D. was thoroughly calibrated and compared to other widely used instruments and methods such asphoto-gravimetry, LiCor Li3100, WinDIAS2.0 and ImageTool. By comparison, the GRA.LE.D. presentedthe most accurate measurements of leaf area, but the LiCor Li3100 and the WinDIAS2.0 were faster, whilethe photo-gravimetric method proved to be the most time-consuming. The WinDIAS2.0 instrument wasthe least reliable. The GRA.LE.D. is uncomplicated, user-friendly, accurate, consistent, reliable and haswide practical application

Vine signal extraction : an application of remote sensing in precision viticulture

The original publication is available at http://www.sasev.org/.This paper presents a study of precision agriculture in the wine industry. While precision viticulture mostly aims to maximise yields by delivering the right inputs to appropriate places on a farm in the correct doses and at the right time, the objective of this study was rather to assess vine biomass differences. The solution proposed in this paper uses aerial imagery as the primary source of data for vine analysis. The first objective to be achieved by the solution is to automatically identify vineyards blocks, vine rows, and individual vines within rows. This is made possible through a series of enhancements and hierarchical segmentations of the aerial images. The second objective is to determine the correlation of image data with the biophysical data (yield and pruning mass) of each vine. A multispectral aerial image is used to compute vegetation indices, which serve as indicators of biophysical measures. The results of the automatic detection are compared against a test field, to verify both vine location and vegetation index correlation with relevant vine parameters. The advantage of this technique is that it functions in environments where active cover crop growth between vines is evident and where variable vine canopy conditions are present within a vineyard block.Publishers' versio

Integrative effects of vine water relations and grape ripeness level of vitis vinifera l. cv. Shiraz/Richter 99. I. physiological changes and vegetative-reproductive growth balances

CITATION: Hunter, J. J., et al. 2014. Integrative effects of vine water relations and grape ripeness level of vitis vinifera l. cv. Shiraz/Richter 99. I. physiological changes and vegetative-reproductive growth balances. South African Journal of Enology & Viticulture, 35(2):332-358, doi:10.21548/35-2-1021.The original publication is available at http://www.journals.ac.za/index.php/sajevThe water relations and physiological status of the grapevine are critical for obtaining a quality product and for fully exploring vineyard and grape potential. The objective of this investigation was to determine the effect of grapevine water status (induced by means of two field water capacity-based irrigation levels, 75% and 100%, applied at single and combined vine developmental stages) on morphological and physiological changes in Vitis vinifera L. cv. Shiraz/Richter 99 grapevines and grapes (harvested at different soluble solid levels) under field conditions. The integrative effects of vine water relations and grape ripeness level, specifically in a Mediterranean high winter rainfall area, have not yet been investigated systematically. The terroir affected the reaction of the vines to treatments. The soil displayed high water-holding capacity and a buffer against favourable evapotranspiration conditions, even with a western aspect and being subjected to long and relatively dry seasons, with frequent occurrence of high temperatures and grapevines with fully developed canopies. The vines did not seem overly stressed – in line with the relatively high base soil water fractions of mostly more than 50% of field water capacity. Primary and secondary leaf water potential and stem water potential displayed similar patterns and the water potential of the primary and secondary leaves was similar. Despite relatively high base soil water contents that prevented excessively low plant water potential and classic leaf and berry behaviour to surface, the vines still responded in a noticeable way to volume and timing of irrigation in relation to the grape ripeness level status. Water relations, ripeness level and terroir conditions showed an integrated, steering impact on physiological, vegetative and reproductive behaviour. Post-véraison irrigated vines were expected to maintain relatively high water potential during the last weeks of the ripening period, but this seemed not to be the case. All vines seemed to have recuperated/stabilised during this time, maintaining their water balances. Physical, physiological and compositional changes in the berry during late ripening under field conditions were clarified further. New information was obtained on the relationships between the behaviour of the root system, canopy and grapes and the changing terroir conditions during the ripening period.http://www.journals.ac.za/index.php/sajev/article/view/1021Publisher's versio

Ecophysiology, vigour, berry and wine characteristics of grapevines growing on and off heuweltjies

CITATION: Bekker, S. J., et al.2016. Ecophysiology, vigour, berry and wine characteristics of grapevines growing on and off heuweltjies. South African Journal of Enology & Viticulture, 37(2):176-192, doi:10.21548/37-2-690.The original publication is available at http://www.journals.ac.za/index.php/sajevHeuweltjies are unique landscape features putatively created by the termite Microhodotermes viator through their burrowing and nest-building activities. They have been closely examined in the natural veld of the Western Cape in the recent past and are the focus of many ecological studies, but their effect in cultivated landscapes (e.g. vineyards and orchards) has remained unexplored. This study addresses the vigour and physiology of vines growing on and off heuweltjies, as well as the wine emanating from these vines. This study was conducted on Cabernet Sauvignon and Shiraz in two climatic regions of the Western Cape, namely Stellenbosch (Mediterranean climate) and Robertson (semi-arid climate) respectively, to better understand how differences in heuweltjie characteristics correspond to differences in rainfall and temperature. Through the use of ANOVAs and Fisher’s LSD post hoc tests to indicate statistical significance, it was apparent that the soil on and off heuweltjies differed significantly in respect of several physical and chemical properties. Consequently, soil water content was more favourable on heuweltjies, especially in the Stellenbosch area, where only supplementary irrigation was applied. Heuweltjies induce substantial changes in grapevine vigour and grape composition. Differences in grapevine physiology between heuweltjie and non-heuweltjie plots were subtle, but vine vigour was severely altered on the heuweltjieassociated vines, exhibiting excessive vegetative growth in Stellenbosch and leading to variations in berry and wine characteristics on and off the heuweltjies. The opposite was observed in the semi-arid climate of Robertson. The presence of heuweltjies in vineyards presents an opportunity to produce and market wines with a difference in respect of their characteristics and unique origin.http://www.journals.ac.za/index.php/sajev/article/view/690Publisher's versio

GRA.LE.D. (GRApevine LEeaf Digitalization) software for the detection and graphic reconstruction of ampelometric differences between Vitis leaves

CITATION: Bodor, P. et al. 2012. GRA.LE.D. (GRApevine LEeaf Digitalization) software for the detection and graphic reconstruction of ampelometric differences between Vitis leaves. South African Journal of Enology and Viticulture, 33(1):1-6, doi:10.21548/33-1-1299.The original publication is available at http://www.journals.ac.za/index.php/sajevRaster graphic ampelometric software was not exclusively developed for the estimation of leaf area, but also for the characterization of grapevine (Viti vinifera L.) leaves. The software was written in C++ programming language, using the C++ Builder 2007 for Windows 95-XP and Linux operation systems. It handles desktop-scanned images. On the image analysed with the GRA.LE.D., the user has to determine 11 points. These points are then connected and the distances between them calculated. The GRA.LE.D. software supports standard ampelometric measurements such as leaf area, angles between the veins and lengths of the veins. These measurements are recorded by the software and exported into plain ASCII text files for single or multiple samples. Twenty-two biometric data points of each leaf are identified by the GRA.LE.D. It presents the opportunity to statistically analyse experimental data, allows comparison of cultivars and enables graphic reconstruction of leaves using the Microsoft Excel Chart Wizard. The GRA. LE.D. was thoroughly calibrated and compared to other widely used instruments and methods such as photo-gravimetry, LiCor Li3100, WinDIAS2.0 and ImageTool. By comparison, the GRA.LE.D. presented the most accurate measurements of leaf area, but the LiCor Li3100 and the WinDIAS2.0 were faster, while the photo-gravimetric method proved to be the most time-consuming. The WinDIAS2.0 instrument was the least reliable. The GRA.LE.D. is uncomplicated, user-friendly, accurate, consistent, reliable and has wide practical application.http://www.journals.ac.za/index.php/sajev/article/view/1299Publisher's versio

Synthesis and characterization of a reconstituted myoglobin-chlorin e6 adduct for theranostic applications

Chlorin e6 (Ce6) and its derivatives are among the most important photosensitizers in photodynamic therapy. Due to their intense fluorescence, chlorins may also be used for diagnostics. However, low solubility in water and high tendency to aggregation restrict their medical use. Here we demonstrate that apo-myoglobin, by reinserting Ce6 in its heme binding pocket, can be used to monomolecularly disperse it. The reconstructed myoglobin-Ce6 adduct presents noticeable changes in the photophysical properties of the chromophore. A red-shift, in particular in the transparency window, can be observed in the absorption and in the emission spectra of the adduct compared to the spectra of the free chlorin in PBS. The adduct presents a higher quantum yield and an increased excited-state lifetime with respect to the free Ce6. The binding of Ce6 to apo-myoglobin determines a decrease of the O-1(2) generation but a three-fold increase of peroxides production, determining globally an increase in the performance of Ce6 as a photosensitizer and imaging agent