7 research outputs found
Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle – an Experimental Study on a Porcine Model
Cleft palate caused by congenital teratoma
International audienceA cleft palate results from incomplete fusion of the lateral palatine processes, the median nasal septum and the median palatine process. This case report describes a rare case of congenital teratoma originating from the nasal septum that may have interfered with the fusion of the palatal shelves during embryonic development, resulting in a cleft palate. An infant girl was born at 40 weeks of gestation weighing 3020 g with a complete cleft palate associated with a large central nasopharyngeal tumour. Computed tomography (CT) of the head showed a well defined mass of mixed density. The tumour was attached to the nasal septum in direct contact with the cleft palate. A biopsy confirmed the teratoma. Tumour resection was performed at 5 months, soft palate reconstruction at 7 months and hard palate closure at 14 months. There was no sign of local recurrence 1 year later. Most teratomas are benign and the prognosis is usually good. However, recurrence is not rare if germ cell carcinomatous foci are present within the teratoma. For these reasons, we advocate the use of a two-stage procedure in which closure of the cleft palate is postponed until histological examination confirms complete excision of the teratoma
Donor Nerve Selection in Free Gracilis Muscle Transfer; Systematic Review and Meta-Analysis
Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle – an Experimental Study on a Porcine Model
The aim of this study was to evaluate the amount of Endothelin-1 (ET-1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET-1 gene expression in pathogenesis of mechanically induced vasospasm. The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs. Laser Doppler was used for peripheral blood flow measurement. Specimens from the vasospastic vessel (group of specimens B) and from the flap border with no vasospasm (control group A) were taken 2 h after the stimulus initiation. Detection of ET-1 mRNA by Quantitative Real-Time RT-PCR was performed. β-actin was selected as an acceptable reference gene. Relative gene expression data were given as the n-fold change in transcription of target genes normalized to the endogenous control. Relative gene expressions and time indicators of vasospasm were compared in both groups. No significant difference of the ET-1 gene expressions was found between groups A and B (p = 0.505). No correlation between the duration of vasospasm and ET-1 gene expression was found as well (p = 0.299). In conclusion, the expression of the ET-1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased. In this study, the causality of the vasospasm pathogenesis and gene expression of ET-1 was not proven
Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels
The ability to tailor mechanical
properties and architecture is
crucial in creating macroporous hydrogel scaffolds for tissue engineering.
In the present work, a technique for the modification of the pore
size and stiffness of acrylamide-based cryogels is demonstrated via
the regulation of an electron beam irradiation dose. The samples were
characterized by equilibrium swelling measurements, light and scanning
electron microscopy, mercury porosimetry, Brunauer–Emmett–Teller
surface area analysis, and stiffness measurements. Their properties
were compared to cryogels prepared by a standard redox-initiated radical
polymerization. A <sup>125</sup>I radiolabeled azidopentanoyl-GGGRGDSGGGY-NH<sub>2</sub> peptide was bound to the surface to determine the concentration
of the adhesive sites available for biomimetic modification. The functionality
of the prepared substrates was evaluated by in vitro cultivation of
adipose-derived stem cells. Moreover, the feasibility of preparing
layered cryogels was demonstrated. This may be the key to the future
preparation of complex hydrogel-based scaffolds to mimic the extracellular
microenvironment in a wide range of applications