17 research outputs found
Imaging cardiac SCN5A using the novel F-18 radiotracer radiocaine
The key function of the heart, a well-orchestrated series of contractions, is controlled by cardiac action potentials. These action potentials are initiated and propagated by a single isoform of voltage gated sodium channels - SCN5A. However, linking changes in SCN5A expression levels to human disease in vivo has not yet been possible. Radiocaine, an F-18 radiotracer for positron emission tomography (PET), is the first SCN5A imaging agent in the heart. Explants from healthy and failing human hearts were compared using radiocaine autoradiography to determine that the failing heart has ~30% lower SCN5A levels - the first evidence of changes in SCN5A expression in humans as a function of disease. Paving the way for translational imaging, radiocaine proved to exhibit high in vivo specific binding to the myocardium of non-human primates. We envision that SCN5A measurements using PET imaging may serve as a novel diagnostic tool to stratify arrhythmia risk and assess for progression of heart failure in patients with a broad spectrum of cardiovascular diseases.status: publishe
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Imaging cardiac SCN5A using the novel F-18 radiotracer radiocaine
The key function of the heart, a well-orchestrated series of contractions, is controlled by cardiac action potentials. These action potentials are initiated and propagated by a single isoform of voltage gated sodium channels – SCN5A. However, linking changes in SCN5A expression levels to human disease in vivo has not yet been possible. Radiocaine, an F-18 radiotracer for positron emission tomography (PET), is the first SCN5A imaging agent in the heart. Explants from healthy and failing human hearts were compared using radiocaine autoradiography to determine that the failing heart has ~30% lower SCN5A levels - the first evidence of changes in SCN5A expression in humans as a function of disease. Paving the way for translational imaging, radiocaine proved to exhibit high in vivo specific binding to the myocardium of non-human primates. We envision that SCN5A measurements using PET imaging may serve as a novel diagnostic tool to stratify arrhythmia risk and assess for progression of heart failure in patients with a broad spectrum of cardiovascular diseases
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18F-Deoxyfluorination of Phenols via Ru π-Complexes
The deficiency of robust and practical methods for 18F-radiofluorination is a bottleneck for positron emission tomography (PET) tracer development. Here, we report the first transition-metal-assisted 18F-deoxyfluorination of phenols. The transformation benefits from readily available phenols as starting materials, tolerance of moisture and ambient atmosphere, large substrate scope, and translatability to generate doses appropriate for PET imaging
<sup>18</sup>F‑Deoxyfluorination of Phenols via Ru π‑Complexes
The deficiency of robust and practical
methods for <sup>18</sup>F-radiofluorination is a bottleneck for positron
emission tomography
(PET) tracer development. Here, we report the first transition-metal-assisted <sup>18</sup>F-deoxyfluorination of phenols. The transformation benefits
from readily available phenols as starting materials, tolerance of
moisture and ambient atmosphere, large substrate scope, and translatability
to generate doses appropriate for PET imaging
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HDAC6 Brain Mapping with [18F]Bavarostat Enabled by a Ru-Mediated Deoxyfluorination
Histone deacetylase 6 (HDAC6) function and dysregulation have been implicated in the etiology of certain cancers and more recently in central nervous system (CNS) disorders including Rett syndrome, Alzheimer’s and Parkinson’s diseases, and major depressive disorder. HDAC6-selective inhibitors have therapeutic potential, but in the CNS drug space the development of highly brain penetrant HDAC inhibitors has been a persistent challenge. Moreover, no tool exists to directly characterize HDAC6 and its related biology in the living human brain. Here, we report a highly brain penetrant HDAC6 inhibitor, Bavarostat, that exhibits excellent HDAC6 selectivity (>80-fold over all other Zn-containing HDAC paralogues), modulates tubulin acetylation selectively over histone acetylation, and has excellent brain penetrance. We further demonstrate that Bavarostat can be radiolabeled with 18F by deoxyfluorination through in situ formation of a ruthenium π-complex of the corresponding phenol precursor: the only method currently suitable for synthesis of [18F]Bavarostat. Finally, by using [18F]Bavarostat in a series of rodent and nonhuman primate imaging experiments, we demonstrate its utility for mapping HDAC6 in the living brain, which sets the stage for first-in-human neurochemical imaging of this important target
<sup>18</sup>F‑Deoxyfluorination of Phenols via Ru π‑Complexes
The deficiency of robust and practical
methods for <sup>18</sup>F-radiofluorination is a bottleneck for positron
emission tomography
(PET) tracer development. Here, we report the first transition-metal-assisted <sup>18</sup>F-deoxyfluorination of phenols. The transformation benefits
from readily available phenols as starting materials, tolerance of
moisture and ambient atmosphere, large substrate scope, and translatability
to generate doses appropriate for PET imaging
<sup>18</sup>F‑Deoxyfluorination of Phenols via Ru π‑Complexes
The deficiency of robust and practical
methods for <sup>18</sup>F-radiofluorination is a bottleneck for positron
emission tomography
(PET) tracer development. Here, we report the first transition-metal-assisted <sup>18</sup>F-deoxyfluorination of phenols. The transformation benefits
from readily available phenols as starting materials, tolerance of
moisture and ambient atmosphere, large substrate scope, and translatability
to generate doses appropriate for PET imaging
Development of a Fluorinated Class‑I HDAC Radiotracer Reveals Key Chemical Determinants of Brain Penetrance
Despite
major efforts, our knowledge about many brain diseases
remains remarkably limited. Epigenetic dysregulation has been one
of the few leads toward identifying the causes and potential treatments
of psychiatric disease over the past decade. A new positron emission
tomography radiotracer, [<sup>11</sup>C]ÂMartinostat, has enabled the
study of histone deacetylase in living human subjects. A unique property
of [<sup>11</sup>C]ÂMartinostat is its profound brain penetrance, a
feature that is challenging to engineer intentionally. In order to
understand determining factors for the high brain-uptake of Martinostat,
a series of compounds was evaluated in rodents and nonhuman primates.
The study revealed the major structural contributors to brain uptake,
as well as a more clinically relevant fluorinated HDAC radiotracer
with comparable behavior to Martinostat, yet longer half-life