186 research outputs found
Preconcentration and frontal electroelution of amino acids for in-line solid-phase extractionācapillary electrophoresis
The application of real-time PCR to the analysis of T cell repertoires
The diversity of T-cell populations is determined by the spectrum of antigen-specific T-cell receptors (TCRs) that are heterodimers of Ī± and Ī² subunits encoded by rearranged combinations of variable (AV and BV), joining (AJ and BJ), and constant region genes (AC and BC). We have developed a novel approach for analysis of Ī² transcript diversity in mice with a real-time PCR-based method that uses a matrix of BV- and BJ-specific primers to amplify 240 distinct BVāBJ combinations. Defined endpoints (Ct values) and dissociation curves are generated for each BVāBJ combination and the Ct values are consolidated in a matrix that characterizes the Ī² transcript diversity of each RNA sample. Relative diversities of BVāBJ combinations in individual RNA samples are further described by estimates of scaled entropy. A skin allograft system was used to demonstrate that dissection of repertoires into 240 BVāBJ combinations increases efficiency of identifying and sequencing Ī² transcripts that are overrepresented at inflammatory sites. These BVāBJ matrices should generate greater investigation in laboratory and clinical settings due to increased throughput, resolution and identification of overrepresented TCR transcripts
Immune response profiling in early rheumatoid arthritis: discovery of a novel interaction of treatment response with viral immunity
Dr. Joseph Goldberger and Pellagra : His Life and Legacy
Laupus Library History Collections & the Department of Bioethics & Interdisciplinary Studies sponsor the History of Medicine Presentations as an educational service for the East Carolina University community. The Library hopes that the speakers and topics selected will promote a greater understanding of the historical and philosophical underpinnings of today's health care disciplines.Laupus Library History Collections & the Department of Bioethics & Interdisciplinary Studie
Detection on polyacrylamide gels of l-glutamic acid decarboxylase activities from Bacillus thuringiensis
Qualitative and quantitative differences between resident peritoneal mononuclear phagocytes of beige and control mice.
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