Lorentz TEM imaging of stripe structures embedded in a soft magnetic matrix

N\'eel walls in soft magnetic NiFe/NiFeGa hybrid stripe structures surrounded by a NiFe film are investigated by high resolution Lorentz transmission electron microscopic imaging. An anti-parallel orientation of magnetization in 1000 nm wide neighboring unirradiated-irradiated stripes is observed by forming high angle domain walls during magnetization reversal. Upon downscaling the stripe structure size from 1000 nm to 200 nm a transition from a discrete domain pattern to an effective magnetic medium is observed for external magnetic field reversal. This transition is associated with vanishing ability of hosting high angle domain walls between adjacent stripes. The investigation also demonstrated the potentiality of Lorentz microscopy to image periodic stripe structures well under micron length-scale.Comment: 7 pages, 6 figure

Studies on Enantioselective Nickel-Catalyzed Hydrocyanation and Chromane Natural Products

Hydrocyanation describes an atom-economic, C-C bond-forming reaction leading to nitriles, which are of high importance as target structures and synthetic intermediates. However, hydrocyanation of non-activated alkenes is less developed than other transition metal-catalyzed transformations, although it opens up valuable opportunities for (asymmetric) organic synthesis. In this work, enantioselective nickel-catalyzed hydrocyanation using TADDOL-derived chiral phosphine-phosphite ligands was investigated in terms of potential improvements, substrate scope and limitations. As a highly attractive substrate class, 1,3-diarylpropenes (homostilbenes) were hydrocyanated for the first time. For this purpose, a library of various substituted homostilbenes was initially synthesized and their hydrocyanation was investigated with respect to chemo-, regio- and enantioselectivity. Notably, high levels of regio-retention (and enantioselectivity) were observed despite the risk of chain-walking. The utility of the developed methodology was demonstrated in the synthesis of a new colchinol. In a separate project, an enantioselective iridium-catalyzed cyclobutanol fragmentation was employed in studies towards the synthesis of an antiplasmodial chromane natural product, and coupling strategies for the introduction of a terpene-derived side chain were investigated

The Impact of Noise and Mismatch on SAR ADCs and a Calibratable Capacitance Array Based Approach for High Resolutions

This paper describes widely used capacitor structures for charge-redistribution (CR) successive approximation register (SAR) based analog-to-digital converters (ADCs) and analyzes their linearity limitations due to kT/C noise, mismatch and parasitics. Results of mathematical considerations and statistical simulations are presented which show that most widespread dimensioning rules are overcritical. For high-resolution CR SAR ADCs in current CMOS technologies, matching of the capacitors, influenced by local mismatch and parasitics, is a limiting factor. For high-resolution medium-speed CR SAR ADCs, a novel capacitance array based approach using in-field calibration is proposed. This architecture promises a high resolution with small unit capacitances and without expensive factory calibration as laser trimming

Laminar air flow reduces particle load in TKA—even outside the LAF panel: a prospective, randomized cohort study

Purpose: Released particles are a major risk of airborne contamination during surgery. The present prospective study investigated the quantitative and qualitative particle load in the operating room (OR) depending on location, time of surgery and use of laminar air flow (LAF) system. Methods: The particle load/m(3) was measured during the implantation of 12 total knee arthroplasties (6 x LAF, 6 x Non-LAF) by using the Met One HHPC 6 + device (Beckmann Coulter GmbH, Germany). Measurement was based on the absorption and scattering of (laser) light by particles and was performed at three different time-points [empty OR, setting up, ongoing operation) at 3 fixed measurement points [OR table (central LAF area), anaesthesia tower (marginal LAF area), surgical image amplifier (outside LAF area)]. Results: Independent of time and location, all measurements showed a significantly higher particle load in the Non-LAF group (p < 0.01). With ongoing surgical procedure both groups showed increasing particle load. While there was a major increase of fine particles (size < 1 mu m) with advancing activity in the LAF group, the Non-LAF group showed higher particle gain with increasing particle size. The lowest particle load in the LAF group was measured at the operating column, increasing with greater distance from the operating table. The Non-LAF group presented a significantly higher particle load than the LAF group at all locations. Conclusion: The use of a LAF system significantly reduces the particle load and therefore potential bacterial contamination regardless of the time or place of measurement and therefore seems to be a useful tool for infection prevention. As LAF leads to a significant decrease of respirable particles, it appears to be a protective factor for the health of the surgical team regardless of its use in infection prevention. Level of evidence: I

HiNO: An Approach for Inferring Hierarchical Organization from Regulatory Networks

BACKGROUND: Gene expression as governed by the interplay of the components of regulatory networks is indeed one of the most complex fundamental processes in biological systems. Although several methods have been published to unravel the hierarchical structure of regulatory networks, weaknesses such as the incorrect or inconsistent assignment of elements to their hierarchical levels, the incapability to cope with cyclic dependencies within the networks or the need for a manual curation to retrieve non-overlapping levels remain unsolved. METHODOLOGY/RESULTS: We developed HiNO as a significant improvement of the so-called breadth-first-search (BFS) method. While BFS is capable of determining the overall hierarchical structures from gene regulatory networks, it especially has problems solving feed-forward type of loops leading to conflicts within the level assignments. We resolved these problems by adding a recursive correction approach consisting of two steps. First each vertex is placed on the lowest level that this vertex and its regulating vertices are assigned to (downgrade procedure). Second, vertices are assigned to the next higher level (upgrade procedure) if they have successors with the same level assignment and have themselves no regulators. We evaluated HiNO by comparing it with the BFS method by applying them to the regulatory networks from Saccharomyces cerevisiae and Escherichia coli, respectively. The comparison shows clearly how conflicts in level assignment are resolved in HiNO in order to produce correct hierarchical structures even on the local levels in an automated fashion. CONCLUSIONS: We showed that the resolution of conflicting assignments clearly improves the BFS-method. While we restricted our analysis to gene regulatory networks, our approach is suitable to deal with any directed hierarchical networks structure such as the interaction of microRNAs or the action of non-coding RNAs in general. Furthermore we provide a user-friendly web-interface for HiNO that enables the extraction of the hierarchical structure of any directed regulatory network. AVAILABILITY: HiNO is freely accessible at http://mips.helmholtz-muenchen.de/hino/