982 research outputs found
Piecewise adiabatic population transfer in a molecule via a wave packet
We propose a class of schemes for robust population transfer between quantum
states that utilize trains of coherent pulses and represent a generalized
adiabatic passage via a wave packet. We study piecewise Stimulated Raman
Adiabatic Passage with pulse-to-pulse amplitude variation, and piecewise
chirped Raman passage with pulse-to-pulse phase variation, implemented with an
optical frequency comb. In the context of production of ultracold ground-state
molecules, we show that with almost no knowledge of the excited potential,
robust high-efficiency transfer is possibleComment: 4 pages, 5 figures. Submitted to Phys. Rev. Let
Flow-Based Single Cell Deposition for High-Throughput Screening of Protein Libraries
The identification and engineering of proteins having refined or novel characteristics is an important area of research in many scientific fields. Protein modelling has enabled the rational design of unique proteins, but high-throughput screening of large libraries is still required to identify proteins with potentially valuable properties. Here we report on the development and evaluation of a novel fluorescent activated cell sorting based screening platform. Single bacterial cells, expressing a protein library to be screened, are electronically sorted and deposited onto plates containing solid nutrient growth media in a dense matrix format of between 44 and 195 colonies/cm2. We show that this matrix format is readily applicable to machine interrogation (<30 seconds per plate) and subsequent bioinformatic analysis (~60 seconds per plate) thus enabling the high-throughput screening of the protein library. We evaluate this platform and show that bacteria containing a bioluminescent protein can be spectrally analysed using an optical imager, and a rare clone (0.5% population) can successfully be identified, picked and further characterised. To further enhance this screening platform, we have developed a prototype electronic sort stream multiplexer, that when integrated into a commercial flow cytometric sorter, increases the rate of colony deposition by 89.2% to 24 colonies per second. We believe that the screening platform described here is potentially the foundation of a new generation of high-throughput screening technologies for proteins
Velocity-selective direct frequency-comb spectroscopy of atomic vapors
We present an experimental and theoretical investigation of two-photon direct
frequency-comb spectroscopy performed through velocity-selective excitation. In
particular, we explore the effect of repetition rate on the
two-photon transitions
excited in a rubidium atomic vapor cell. The transitions occur via step-wise
excitation through the states by use of the direct
output of an optical frequency comb. Experiments were performed with two
different frequency combs, one with a repetition rate of MHz and
one with a repetition rate of MHz. The experimental spectra are
compared to each other and to a theoretical model.Comment: 10 pages, 7 figure
Laser-Induced Plasma Analysis for Surrogate Nuclear Debris
This work identifies analytical lines in laser-induced plasma for chemical analyses of major elements found in surrogate nuclear debris. These lines are evaluated for interferences and signal strength to insure they would be useful to measure relative concentrations. Compact, portable instruments are employed and can be included as part of a mobile nuclear forensics laboratory for field screening of nuclear debris and contamination. The average plasma temperature is measured using the well-established Boltzmann plot technique, and plasma\u27s average electron density is determined using empirical formulae based on Stark broadening of the H-alpha line. These measurements suggest existence of partial local thermal equilibrium
Complete transfer of populations from a single state to a pre-selected superposition of states using Piecewise Adiabatic Passage
We develop a method for executing robust and selective transfer of
populations between a single level and pre-selected superpositions of energy
eigenstates. Viewed in the frequency domain, our method amounts to executing a
series of simultaneous adiabatic passages into each component of the target
superposition state. Viewed in {the} time domain, the method works by
accumulating the wavefunction of the target wave packet as it revisits the
Franck Condon region, in what amounts to an extension of the Piecewise
Adiabatic Passage technique [ Shapiro et.al., Phys. Rev. Lett. 99, 033002
(2007)] to the multi-state regime. The viability of the method is verified by
performing numerical tests for the Na_2 molecule.Comment: 8 pages, 4 figure
Hydrogen Motion in Magnesium Hydride by NMR
In coarse-grained MgH2, the diffusive motion of hydrogen remains too slow (<10^5 hops s^−1) to narrow the H NMR line up to 400 °C. Slow-motion dipolar relaxation time T1D measurements reveal the motion, with hopping rate ωH from 0.1 to 430 s^−1 over the range of 260 to 400 °C, the first direct measurement of H hopping in MgH2. The ωH data are described by an activation energy of 1.72 eV (166 kJ/mol) and attempt frequency of 2.5 × 10^15 s^−1. In ball-milled MgH2 with 0.5 mol % added Nb2O5 catalyst, line-narrowing is evident already at 50 °C. The line shape shows distinct broad and narrow components corresponding to immobile and mobile H, respectively. The fraction of mobile H grows continuously with temperature, reaching ∼30% at 400 °C. This demonstrates that this material’s superior reaction kinetics are due to an increased rate of H motion, in addition to the shorter diffusion paths from ball-milling. In ball-milled MgH2 without additives, the line-narrowed component is weaker and is due, at least in part, to trapped H2 gas. The spin−lattice relaxation rates T1^−1 of all materials are compared, with ball-milling markedly increasing T1^−1. The weak temperature dependence of T1^−1 suggests a mechanism with paramagnetic relaxation centers arising from the mechanical milling
"Out here the rainbow has mud on it": Amenability of mobile technology based EMA to assess high risk behaviors among rural MSM
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Temperature-Programmed Desorption: Principles, Instrument Design, and Demonstration With NaAlH4
This article is a brief introduction to temperature-programmed desorption (TPD), an analytical technique devised to analyze, in this case, materials for their potential as hydrogen storage materials. The principles and requirements of TPD are explained and the different components of a generic TPD apparatus are described. The construction of a modified TPD instrument from commercially available components is reported together with the control and acquisition technique used to create a TPD spectrum. The chemical and instrumental parameters to be considered in a typical TPD experiment and the analytical utility of the technique are demonstrated by the dehydrogenation of titanium-doped NaAlH{sub 4} by means of thermally programmed desorption
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