Coral of Opportunity Survivorship and the Use of Coral Nurseries in Coral Reef Restoration

Coral reef damage is unfortunately becoming a common occurrence off southeast Florida, U.S.A. Reattachment of the dislodged scleractinian corals usually initiates damage site restoration. Because mortality of dislodged colonies is typically high and natural recovery in southeast Florida is typically slow, transplantation of additional scleractinian corals into a damaged area has been used to accelerate reef recovery. Donor colonies available for transplantation have been grown in situ, grown in laboratories, and taken from nondamaged reef areas. An alternative source of donor colonies for transplantation into damaged sites is “corals of opportunity,” which we define as scleractinian corals that have been detached from the reef through natural processes or unknown events. This paper describes a project, initiated in 2001 in Broward County, Florida, that was developed to collect these dislodged colonies and transplant them to a coral nursery. Coral nurseries are interim locations that function as storage sites for corals of opportunity where they can be cached, stabilized, and allowed to grow, until needed as donor colonies for future restoration activities. This project is a partnership between a local university, county government, and a volunteer dive group. Two hundred and fifty corals of opportunity were collected, transplanted to the coral nurseries, and monitored for survival. Transplanted colony survival was similar to that of naturally attached control colonies and significantly greater than that of corals of opportunity left unattached. Results provide resource managers with information on the utility of using corals of opportunity as a source of transplant donor colonies, and the value of using coral nurseries to create a reserve of corals of opportunity for use in future coral reef restoration activities

Florida Coral Reef Damage from Nuclear Submarine Grounding and Proposed Restoration

The United States submarine Memphis grounded in approximately 10 m water depth on a tropical coral reef ofT southeast Florida in February, 1993. The grounding caused extensive physical and biological damage to the reef substrate and to the coral community. As part of a claim by the State of Florida against the United States, the impact of the grounding was assessed, and the area of damage was determined through field and photographic studies. A recovery rate for the reef was assigned from literature estimates. The NOAA Habitat Equivalency Model (HEM) was used to calculate the reef area needed to be replaced in order to compensate for damages. A plan devised to restore the reef included: removal of loose rubble generated from the grounding; stabilization of reef faces in danger of collapse: emplacement of six different types of artificial reefs; transplantation of reef-building corals (15% of the number damaged) to bare damaged substrate and to the artificial reefs; and a 20 year monitoring period to assess restoration plan efficacy. Settlement of the claim in April, 1997 resulted in an award of $750,000 to the Ecosystem Management Trust Fund of the State of Florida. Utilization of this fund necessitates a revised plan to restore the damaged reef within economic constraints. This plan will involve rubble removal/stabilization, artificial reef emplacement, stony coral transplantation, and monitoring

Real-Time Coral Stress Observations Before, During, and After Beach Nourishment Dredging Offshore SE Florida

Beach nourishment in Southeast Florida involves dredging sand source borrow areas located between offshore reefs. From May 2005 to February 2006 Broward County, FL. nourished 10.9 km of beach with 1.5 ×106 m3 of sand. As part of a program to monitor potential reef community impacts, a visual stress index was developed from laboratory experiments and histological analyses for three stony coral species (Montastrea cavernosa, Solenastrea bournoni, and Siderastrea siderea). Scoring involved healthy = 0; moderately stressed = 1 (polyp swelling, increased mucus); markedly stressed = 2 (coloration changes, increased mucus secretion, tissue thinning); and severely stressed = 3 (severe swelling/thinning tissue erosion/necrosis). Colonies were scored weekly at sites adjacent to borrow areas and control sites pre-, during, and post-dredging. Permit conditions were established which would suspend dredging based on mean stress index values above 1.5 at 50% of monitored sites adjacent to borrow areas. This condition was never met. However, three hurricanes, passing the region during dredging, contributed to an elevated mean stress level above 1.0. Post-dredging observations documented recovery to pre-dredging stress levels. This program was effectively used to monitor stress on a sensitive marine habitat adjacent to sediment dredging activities

Regulation of the Phonotactic Threshold of the Female Cricket, Acheta domesticus: Juvenile Hormone III, Allatectomy, L1 Auditory Neuron Thresholds and Environmental Factors

Juvenile hormone III (JHIII), when applied to the abdomen of 1-day-old female Acheta domesticus (in quantities that would create JHIII titers in the hemolymph that were within the range measured in females of this species) caused a significant decrease in phonotactic thresholds (Fig. 1). Removal of the corpora allata from 5-day-old females with low phonotactic thresholds caused significantly increased phonotactic thresholds 2-5 days later. After a temporary increase (24 h) of, on average, about 25 dB, the phonotactic thresholds drop to about 10 dB above preallatectomy levels (Fig. 2), but remain significantly higher than controls. Application of JHIII to allatectomized females, with a mean increase in thresholds of 20 dB, results in significantly decreased thresholds (mean of about 20 dB) over the next 6 h (Fig. 3). Exposure to males 1 week before the imaginal molt causes the phonotactic thresholds of postimaginal females to drop 1-2 days significantly earlier than controls (Fig. 4). One- and 3-day-old females, phonotactically tested only once, exhibit lower thresholds in the early morning than they do in the late afternoon (Fig. 5). Five-day-old females do not exhibit such a diurnal rhythm. Phonotactically testing females more than once a day significantly influences their phonotactic thresholds (Figs. 6, 7). In 1-day-old females, with high (above 70 dB) phonotactic thresholds, the threshold of their L1 auditory interneurons can be 30 dB or more below their phonotactic threshold (Fig. 8). In females with phonotactic thresholds of 70 dB or lower, the L1 threshold is within 10 dB of their phonotactic threshold. Both JHIII and allatectomy influence phonotactic and L1 thresholds in a similar manner

Reliability and Validity of the HD-PRO-TriadTM, a Health-Related Quality of Life Measure Designed to Assess the Symptom Triad of Huntington\u27s Disease.

BACKGROUND: Huntington\u27s disease (HD), is a neurodegenerative disorder that is associated with cognitive, behavioral, and motor impairments that diminish health related quality of life (HRQOL). The HD-PRO-TRIADTM is a quality of life measure that assesses health concerns specific to individuals with HD. Preliminary psychometric characterization was limited to a convenience sample of HD participants who completed measures at home so clinician-ratings were unavailable. OBJECTIVES: The current study evaluates the reliability and validity of the HD-PRO-TRIADTM in a well-characterized sample of individuals with HD. METHODS: Four-hundred and eighty-two individuals with HD (n = 192 prodromal, n = 193 early, and n = 97 late) completed the HD-PRO-TRIADTM questionnaire. Clinician-rated assessments from the Unified Huntington Disease Rating Scales, the short Problem Behaviors Assessment, and three generic measures of HRQOL (WHODAS 2.0, RAND-12, and EQ-5D) were also examined. RESULTS: Internal reliability for all domains and the total HD-PRO-TRIADTM was excellent (all Cronbach\u27s α \u3e0.93). Convergent and discriminant validity were supported by significant associations between the HD-PRO-TRIADTM domains, and other patient reported outcome measures as well as clinician-rated measures. Known groups validity was supported as the HD-PRO-TRIADTM differentiated between stages of the disease. Floor and ceiling effects were generally within acceptable limits. There were small effect sizes for 12-month change over time and moderate effect sizes for 24-month change over time. CONCLUSIONS: Findings support excellent internal reliability, convergent and discriminant validity, known groups validity, and responsiveness to change over time. The current study supports the clinical efficacy of the HD-PRO-TRIADTM. Future research is needed to assess the test-retest reliability of this measure

Agreement Between Clinician-Rated Versus Patient-Reported Outcomes in Huntington Disease

BACKGROUND: Clinician-rated measures of functioning are often used as primary endpoints in clinical trials and other behavioral research in Huntington disease. As study costs for clinician-rated assessments are not always feasible, there is a question of whether patient self-report of commonly used clinician-rated measures may serve as acceptable alternatives in low risk behavioral trials. AIM: The purpose of this paper was to determine the level of agreement between self-report and clinician-ratings of commonly used functional assessment measures in Huntington disease. DESIGN: 486 participants with premanifest or manifest Huntington disease were examined. Total Functional Capacity, Functional Assessment, and Independence Scale assessments from the Unified Huntington Disease Rating scale were completed by clinicians; a self-report version was also completed by individuals with Huntington disease. Cronbach\u27s α was used to examine internal consistency, one-way analysis of variance was used to examine group differences, and paired t tests, kappa agreement coefficients, and intra-class correlations were calculated to determine agreement between raters. RESULTS: Internal consistency for self-reported ratings of functional capacity and ability were good. There were significant differences between those with premanifest, early-, and late-stage disease; those with later-stage disease reported less ability and independence than the other clinical groups. Although self-report ratings were not a perfect match with associated clinician-rated measures, differences were small. Cutoffs for achieving specified levels of agreement are provided. CONCLUSIONS: Depending on the acceptable margin of error in a study, self-reported administration of these functional assessments may be appropriate when clinician-related assessments are not feasible

Evaluating Cognition in Individuals with Huntington Disease: NeuroQoL Cognitive Functioning Measures

PURPOSE: Cognitive functioning impacts health-related quality of life (HRQOL) for individuals with Huntington disease (HD). The Neuro-QoL includes two patient-reported outcome (PRO) measures of cognition-Executive Function (EF) and General Concerns (GC). These measures have not previously been validated for use in HD. The purpose of this analysis is to evaluate the reliability and validity of the Neuro-QoL Cognitive Function measures for use in HD. METHODS: Five hundred ten individuals with prodromal or manifest HD completed the Neuro-QoL Cognition measures, two other PRO measures of HRQOL (WHODAS 2.0 and EQ5D), and a depression measure (PROMIS Depression). Measures of functioning The Total Functional Capacity and behavior (Problem Behaviors Assessment) were completed by clinician interview. Objective measures of cognition were obtained using clinician-administered Symbol Digit Modalities Test and the Stroop Test (Word, Color, and Interference). Self-rated, clinician-rated, and objective composite scores were developed. We examined the Neuro-QoL measures for reliability, convergent validity, discriminant validity, and known-groups validity. RESULTS: Excellent reliabilities (Cronbach\u27s alphas ≥ 0.94) were found. Convergent validity was supported, with strong relationships between self-reported measures of cognition. Discriminant validity was supported by less robust correlations between self-reported cognition and other constructs. Prodromal participants reported fewer cognitive problems than manifest groups, and early-stage HD participants reported fewer problems than late-stage HD participants. CONCLUSIONS: The Neuro-QoL Cognition measures provide reliable and valid assessments of self-reported cognitive functioning for individuals with HD. Findings support the utility of these measures for assessing self-reported cognition

High content live cell imaging for the discovery of new antimalarial marine natural products

<p>Abstract</p> <p>Background</p> <p>The human malaria parasite remains a burden in developing nations. It is responsible for up to one million deaths a year, a number that could rise due to increasing multi-drug resistance to all antimalarial drugs currently available. Therefore, there is an urgent need for the discovery of new drug therapies. Recently, our laboratory developed a simple one-step fluorescence-based live cell-imaging assay to integrate the complex biology of the human malaria parasite into drug discovery. Here we used our newly developed live cell-imaging platform to discover novel marine natural products and their cellular phenotypic effects against the most lethal malaria parasite, <it>Plasmodium falciparum</it>.</p> <p>Methods</p> <p>A high content live cell imaging platform was used to screen marine extracts effects on malaria. Parasites were grown <it>in vitro </it>in the presence of extracts, stained with RNA sensitive dye, and imaged at timed intervals with the BD Pathway HT automated confocal microscope.</p> <p>Results</p> <p>Image analysis validated our new methodology at a larger scale level and revealed potential antimalarial activity of selected extracts with a minimal cytotoxic effect on host red blood cells. To further validate our assay, we investigated parasite's phenotypes when incubated with the purified bioactive natural product bromophycolide A. We show that bromophycolide A has a strong and specific morphological effect on parasites, similar to the ones observed from the initial extracts.</p> <p>Conclusion</p> <p>Collectively, our results show that high-content live cell-imaging (HCLCI) can be used to screen chemical libraries and identify parasite specific inhibitors with limited host cytotoxic effects. All together we provide new leads for the discovery of novel antimalarials.</p