Cooperative regulation of extracellular signal-regulated kinase activation and cell shape change by filamin A and beta-arrestins.: FLNA AND ßarr COOPERATE TO REGULATE ERK AND CELL SHAPE

14 pagesbeta-Arrestins (betaarr) are multifunctional adaptor proteins that can act as scaffolds for G protein-coupled receptor activation of mitogen-activated protein kinases (MAPK). Here, we identify the actin-binding and scaffolding protein filamin A (FLNA) as a betaarr-binding partner using Son of sevenless recruitment system screening, a classical yeast two-hybrid system, coimmunoprecipitation analyses, and direct binding in vitro. In FLNA, the betaarr-binding site involves tandem repeat 22 in the carboxyl terminus. betaarr binds FLNA through both its N- and C-terminal domains, indicating the presence of multiple binding sites. We demonstrate that betaarr and FLNA act cooperatively to activate the MAPK extracellular signal-regulated kinase (ERK) downstream of activated muscarinic M1 (M1MR) and angiotensin II type 1a (AT1AR) receptors and provide experimental evidence indicating that this phenomenon is due to the facilitation of betaarr-ERK2 complex formation by FLNA. In Hep2 cells, stimulation of M1MR or AT1AR results in the colocalization of receptor, betaarr, FLNA, and active ERK in membrane ruffles. Reduction of endogenous levels of betaarr or FLNA and a catalytically inactive dominant negative MEK1, which prevents ERK activation, inhibit membrane ruffle formation, indicating the functional requirement for betaarr, FLNA, and active ERK in this process. Our results indicate that betaarr and FLNA cooperate to regulate ERK activation and actin cytoskeleton reorganization

β-Arrestin–dependent activation of Ca2+/calmodulin kinase II after β1–adrenergic receptor stimulation

β-Arrestin functions as a scaffold for CaMKII and the Rap guanine nucleotide exchange factor Epac to regulate signaling from β1-ARs

De la " pratique " à la chaire universitaire : l’enseignement de la procédure civile au tournant des XVIIIe et XIXe siècles

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Projet de reconstruction des halles centrales de Paris / par Mr Storez arch[itec]te, anc[ie]n inspecteur aux Travaux Publics

Échelle(s) : [Sans échelle (exprimée)

Introduction

« Histoire de la mémoire judiciaire ». Audace ou témérité ? Toute la question réside dans la définition des termes. Histoire de la mémoire judiciaire n’est pas histoire du champ judiciaire, dont la sphère embrasse une infinité de matières. En un temps où la mémoire est devenue un devoir et, fréquemment, un synonyme de l’histoire, la consultation d’ouvrages lexicologiques ou encyclopédiques, pour être méthodologiquement bien ordinaire, ne s’en impose que davantage. Le classique Dictionnaire de..

De la " pratique " à la chaire universitaire : l’enseignement de la procédure civile au tournant des XVIIIe et XIXe siècles

International audienceRésumé à veni

Identification de nouveaux partenaires d'interaction des b-arrestines (1-oligomérisation des b-arrestines)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF