Is there a relationship between the expression of β-catenin and Ki-67 in canine melanocytic neoplasms? Existe relação entre a expressão de β-catenina e Ki-67 em neoplasias melanocíticas caninas?

An immunohistochemical evaluation of 26 cutaneous and oral benign and malignant canine melanocytic neoplasms was performed to identify a possible relationship between the expression of the β-catenin molecule with cell proliferation using Ki-67 expression. This molecule is a component of the Wnt/β-catenin signalling pathway, which causes a cascade of intracellular events that activate transduction genes and nuclear transcription. The microscopic evaluation was performed considering the β-catenin labelling site (cytoplasmic, nuclear or mixed). The mitotic index was evaluated by the expression of Ki-67 in 10 high power fields (HPF) (400x). Statistically significant difference was neither detected between the β-catenin labelling sites and the various neoplasms, nor a correlation between the β-catenin molecule and the cell proliferation marker Ki-67 in the oral or cutaneous, benign or malignant neoplasms. Our study brings interesting findings and points to future research on this topic, especially with established variables in the clinical, pathological and immunohistochemical fields

Síndrome de Stewart-Treves no membro inferior com apresentação exuberante: Relato de caso

A síndrome de Stewart-Treves é uma rara condição onde um angiossarcoma se desenvolve sobre área de linfedema crônico. Afeta mais frequentemente os membros superiores e representa 5% dos casos de angiossarcoma. Apesar de ser mais comum em mulheres submetidas a mastectomia radical e esvaziamento axilar por neoplasia de mama, pode ocorrer a partir de linfedema de outras etiologias. Clinicamente as lesões são caracterizadas por nódulos únicos ou múltiplos de cor avermelhada ou azulada no estágio inicial e em estágio avançado podem evoluir para massas hemorrágicas e até gangrena. O prognóstico é ruim e a conduta cirúrgica com excisão ampla ou amputação oferecem uma maior chance de sobrevida. Relata-se um caso de Síndrome de Stewart-Treves na perna direita, portanto sem associação com neoplasia mamária e mastectomia, se apresentando de forma exuberante e com acometimento de linfonodos pélvicos e abdominais

Immunophenotypic characterization of lymphocytic infiltration in canine melanocytic tumors / Caracterização imunofenotípica da infiltração linfocítica em tumores melanocíticos caninos

Melanocytic tumors correspond to approximately for 4 to 7% of tumors in dogs and up to 7% of malignant tumours. Melanomas generally exhibit aggressive biological behavior. In humans, abundant tumor-infiltrating lymphocytes (TIL) can be considered a good prognostic factor. Research on TIL in canine melanomas is scarce, and to date, there are no studies to verify its association with established prognostic factors. Our study aimed to evaluate the lymphocyte population in canine melanocytic tumors using immunohistochemical markers, and to relate this to pre-established clinicopathological prognostic variables and cell proliferation index (Ki-67). An exploratory cross-sectional study was conducted with 21 cases in 19 dogs, of which 71.5% were melanotic melanomas, 19% were melanocytomas and 9.5% were amelanotic melanomas. Ki-67 expression was elevated in amelanotic melanomas, indicating aggressive tumor behavior. We detected lymphocytes T and B through CD3 and CD20 markers, respectively, and found that 86.7% of the melanotic melanomas were positive for CD3, whereas 73.3% were negative for CD20. TIL showed a strong association with malignant tumors, as well as a correlation with other pre-established prognostic factors, such as necrosis, ulceration, and nuclear atypia. TIL need to be further investigated to verify its inclusion as a prognostic factor for canine melanomas

Síndrome de Stewart-Treves no membro inferior com apresentação exuberante: Relato de caso

A síndrome de Stewart-Treves é uma rara condição onde um angiossarcoma se desenvolve sobre área de linfedema crônico. Afeta mais frequentemente os membros superiores e representa 5% dos casos de angiossarcoma. Apesar de ser mais comum em mulheres submetidas a mastectomia radical e esvaziamento axilar por neoplasia de mama, pode ocorrer a partir de linfedema de outras etiologias. Clinicamente as lesões são caracterizadas por nódulos únicos ou múltiplos de cor avermelhada ou azulada no estágio inicial e em estágio avançado podem evoluir para massas hemorrágicas e até gangrena. O prognóstico é ruim e a conduta cirúrgica com excisão ampla ou amputação oferecem uma maior chance de sobrevida. Relata-se um caso de Síndrome de Stewart-Treves na perna direita, portanto sem associação com neoplasia mamária e mastectomia, se apresentando de forma exuberante e com acometimento de linfonodos pélvicos e abdominais

Primary malignant neoplasms of the cecal appendix: unusual findings in acute appendicitis

Introduction: To evaluate the prevalence of appendix neoplasia correlating withpatient profile, histological types and frequency.Methods: Data collection was performed in the Pathology Department of a GeneralHospital, with the objective of identifying patients diagnosed with malignant cecalappendix tumors by histopathologic study of specimens from acute appendicitis.Results: The prevalence of malignant primary epithelial neoplasia of the appendixwas 1%. Fifty percent of the cases were neuroendocrine tumors, 35% were mucinous,and 15% were adenocarcinomas. The mean age at diagnosis was 41.3 (SD, 20.4)years (range16-81), with a women/men ratio of 3:1.Discussion: Appendiceal neoplasms are rare and should be suspected manlyin women over 40 years of age with suggestive symptoms of acute appendicitis.The size, location, extent, margins and presence of mucin are essential findings forthe treatment of these patients

Primary malignant neoplasms of the cecal appendix: unusual findings in acute appendicitis

Introduction: To evaluate the prevalence of appendix neoplasia correlating withpatient profile, histological types and frequency.Methods: Data collection was performed in the Pathology Department of a GeneralHospital, with the objective of identifying patients diagnosed with malignant cecalappendix tumors by histopathologic study of specimens from acute appendicitis.Results: The prevalence of malignant primary epithelial neoplasia of the appendixwas 1%. Fifty percent of the cases were neuroendocrine tumors, 35% were mucinous,and 15% were adenocarcinomas. The mean age at diagnosis was 41.3 (SD, 20.4)years (range16-81), with a women/men ratio of 3:1.Discussion: Appendiceal neoplasms are rare and should be suspected manlyin women over 40 years of age with suggestive symptoms of acute appendicitis.The size, location, extent, margins and presence of mucin are essential findings forthe treatment of these patients