Uticaj različitih oblika kalcijum-hidroksida i hlorheksidina kao interseansnih medikamenata na ishod endodontskog lečenja zuba sa hroničnim periapeksnim lezijama

Introduction/Objective The aim of this study was to determine clinical and radiographic periapical healing of teeth with apical periodontitis treated with different formulations of calcium hydroxide (CH) - paste (CH-paste) and gutta-percha points (CH-GP) - as well as those of chlorhexidine (CHX) - gel (CHXgel) and gutta-percha points (CHX-GP) -12 months after therapy. Methods Eighty patients with chronic apical periodontitis were randomly allocated to four treatment groups according to the intracanal medicament used: CH-paste, CH-GP, CHX-gel, and CHX-GP group. Seventy-eight patients were analyzed clinically and radiographically 12 months postoperatively. The periapical index (PAI) was used for the radiographic evaluation of treatment. Results Overall outcome was classified according to radiographic evaluation only, since clinical success was observed in all the patients. In all the groups, significant reduction in PAI scores was observed (p lt 0.001). The proportions of healed teeth (PAI ≤ 2) were 73.7%, 60%, 68.4%, and 65% in CH-paste, CH-GP, CHX-gel and CHX-GP group, respectively, with no significant differences between the groups. Conclusion The results suggest that there are no differences between investigated CH- and CHX-delivery systems regarding treatment outcome of teeth with apical periodontitis.Uvod/Cilj Cilj ovog istraživanja je bio da se ispita klinički i radiografski ishod lečenja zuba sa apeksnim periodontitisom 12 meseci posle završene terapije i primene različitih oblika kalcijum-hidroksida (KH): pasta (KH-pasta) i gutaperka poeni (KH-GP) i hlorheksidina (HH): gel (HH-gel) i gutaperka poeni (HH-GP). Metode Randomizovano je 80 ispitanika sa hroničnim periapeksnim lezijama u četiri grupe na osnovu vrste korišćenog interseansnog medikamenta: KH-pasta, KH-GP, HH-gel i HH-GP. Dvanaest meseci posle završenog lečenja pregledano je 78 ispitanika i urađeni su retroalveolarni snimci. Za procenu radiografskog uspeha lečenja korišćen je periapikalni indeks (PI). Rezultati Ishod lečenja je klasifikovan na osnovu radiološkog nalaza jer je kod svih ispitanika zabeležen klinički uspeh lečenja. U svim ispitivanim grupama je zabeleženo značajno smanjenje vrednosti PI (p lt 0,001). Izlečenje (PI ≤ 2) uočeno je kod 73,3% zuba u grupi KH-pasta, 60% u KH-GP grupi, 68,4% u grupi HH-gel i kod 65% zuba u grupi HH-GP, pri čemu razlike između grupa nisu bile statistički značajne. Zaključak Rezultati ovog istraživanja pokazuju da ne postoji razlika u ishodu lečenja zuba sa apeksnim periodontitisom posle primene ispitivanih oblika KH i HH

Participation of environmental taxes in government expenditures for environmental protection: the case of selected EU countries

The aim of this paper is to show whether the current system of environmental taxation and environmental policies of the states is efficient for solving environmental problems. We analyze the link between environmental tax revenues and total government expenditure as independent variables and government expenditures for environmental protection as dependent variable in selected EU countries. By the implementation of panel data regression analysis on a defined sample, we came to the conclusion that among the observed variables there is a positive relationship. However, this relationship shows that the growth of expenditures for environmental protection is slower than the growth of total expenditure. The results also suggest that the relationship between revenues from environmental taxes and expenditures of the state in the area of environmental protection is a little weaker. With respect to the result of analysis, it can be concluded that the existing state policy in is not sufficiently effective

Prevalencija bakterija Enterococcus faecalis i Porphyromonas gingivalis u inficiranim kanalima korena zuba i njihova osetljivost na endodontsko lečenje - molekularna studija

Introduction Because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. Objective The prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals was studied Also, the effect of endodontic therapy by using intracanal medicaments, calcium hydroxide paste (CH) or gutta-percha points containing calcium hydroxide (CH-GP) or chlorhexidine (CHX-GP) on these microorganisms was assessed by polymerase chain reaction (PCR) assay. Methods Fifty-one patients with chronic apical periodontitis were randomly allocated in one of the following groups according to the intracanal medicament used: CH, CH-GP and CHX-GP group. Bacterial samples were taken upon access (S1), after chemomechanical instrumentation (S2) and after 15-day medication (S3). PCR assay was used to detect the presence of selected bacteria. Results E. faecalis was detected in 49% (25/51) and P. gingivalis in 17.6% (9/51) of the samples. Samples which showed no bacterial presence at S1 were excluded from further analysis. Overall analysis of all 29 samples revealed significant differences between S1 and S2 (p lt 0.001), S2 and S3 (p lt 0.05), and S1 and S3 (p lt 0.001). When distinction was made between the intracanal medications, there was a significant difference in the number of PCR positive samples between S1 and S2, S1 and S3, but not between S2 and S3 samples. Conclusion E. faecalis is more prevalent than P. gingivalis in primary endodontic infection. Intracanal medication in conduction with instrumentation and irrigation efficiently eliminates E. faecalis and P. gingivalis from infected root canals.Uvod Budući da je apeksni parodontitis oboljenje koje nastaje usled postojanja infekcije, za postizanje optimalnog ishoda lečenja najznačajnije je uklanjanje bakterija iz kanala korena zuba ili barem smanjenje njihovog broja. Cilj rada Cilj ovog rada je bio da se ispita prevalencija bakterija Enterococcus faecalis i Porphyromonas gingivalis u inficiranim kanalima korena zuba i ustanovi efekat endodontskog lečenja primenom intrakanalnih medikamenata - kalcijum-hidroksidne suspenzije (CH), gutaperka-poena na bazi kalcijum-hidroksida (CH-GP) ili gutaperka-poena na bazi hlorheksidina (CHX-GP) - na ove mikroorganizme metodom lančane reakcije polimerizacije (PCR). Metode rada Istraživanjem je obuhvaćena 51 osoba koja je imala zub s hroničnim apeksnim parodontitisom. Ispitanici su metodom slučajnog uzorka svrstani u tri grupe u zavisnosti od vrste primenjenog intrakanalnog medikamenta (CH, CH-GP i CHX-GP). Bakterijski uzorci iz kanala korena sakupljani su pri inicijalnom ulasku u kanal korena (S1), posle hemomehaničke obrade (S2) i posle petnaestodnevnog lečenja (S3). PCR analiza je korišćena za određivanje prisustva ispitivanih bakterija. Rezultati E. faecalis je izolovan iz 25 (49%) uzoraka, a P. gingivalis iz devet (17,6%). Uzorci u kojima nisu nađene bakterije u S1 fazi isključeni su iz dalje analize. Analiza svih 29 uzoraka je ukazala na statistički značajnu razliku između S1 i S2 uzoraka (p lt 0,001), S2 i S3 (p lt 0,05) i S1 i S3 (p lt 0,001). Kada se uzme u obzir vrsta intrakanalnog medikamenta, statistički značajna razlika u broju PCR-pozitivnih uzoraka zabeležena je između S1 i S2, S1 i S3, ali ne i između S2 i S3. Zaključak U primarnoj endodontskoj infekciji E. faecalis se češće javlja od P. gingivalis. Intrakanalna medikacija zajedno s instrumentacijom i irigacijom efikasno uklanja E. faecalis i P. gingivalis iz inficiranih kanala korena

Effect of iron supplementation on maximal oxygen uptake in female athletes

Background/Aim. Iron is a vital constituent of hemoglobin, myoglobin, and some mitochondrial enzymes; therefore, body iron deficiency may result in reduced aerobic capacity. The aim of this study was to evaluate the effects of daily oral iron supplementation on body iron status, and the maximal oxygen uptake (VO2max) in female athletes with latent iron deficiency, as well as with irondeficiency anemia. Methods. A total of 37 female volleyball players were included in the study. Seventeen female athletes had latent iron deficiency, and 20 ones iron deficiency anemia. Both groups were divided into the experimental and the control group. The experimental groups received a daily oral iron supplement (200 mg ferrous sulfate), for a two-month training course. Iron status was determined by serum parameters as follows: red blood cells count, hemoglobin concentration, serum iron and ferritin levels, an unsaturated iron binding capacity, total iron binding capacity and transferrin saturation. VO2max was determined by an indirect test. Results. Statistical difference between the latent iron deficient group versus the iron deficient anemic group was found regarding VO2max (p < 0.001). There were correlations between hemoglobin concentration and VO2max in the latent iron deficient group, as well as in the iron deficient anaemic group (p < 0.05). After two months, there was a significant increase in VO2max in all groups (from 7.0% to 18.2%). Values of VO2max at the end of training period were significantly different (45.98 ± 1.76 vs 42.40 ± 1.22 mL/kg/min; p <0.001) between the experimental and the control group only in female athletes with iron deficiency anemia. After the supplementation, markers of iron status were significantly higher in supplemented groups than in the controls. Conclusion. VO2max was significantly lower in the iron deficient anemic group versus the latent iron deficient group. Iron supplementation during a two-month training period significantly improved body iron status in the iron deficient female athletes with or without anemia, and significantly increased VO2max only in the subjects with iron deficiency anemia

Kinetika fermentacije i fizičkohemijske karakteristike specijalnih piva sa dodatkom grožđa sorte Prokupac

Over the last decade, the market of special beers with improved healthy function and/or with new refreshing taste has significantly increased. One of the possible solutions enables mixing beer with bioactive components in grapes responsible for well-known health-promoting action of red wine. The effects of the addition of the Prokupac grape on the physicochemical properties and the fermentation kinetics of the grape beer were studied and the results were compared with a control lager beer. The effect of grape addition on the activity of yeast was also studied. Original extract, alcohol content, degree of fermentation, fermentation rate and yeast growth were significantly higher in beers with grapes as a consequence of higher concentration of simple sugars in grapes compared with pure wort. Based on the CIELab chromatic parameters the color of grape beer samples was yellow with certain proportion of redness, while the control beer was purely yellow. The increase in the concentration of grape mash affects the reduction of lightness and yellowness of beers, while the redness of samples was directly proportional with grape quantity. The phenolic content and antioxidant capacity of grape beers was remarkably higher compared to the control beer, which indicates that the grape beer is a better source of natural antioxidants than regular lager beer.Poslednjih godina, tržište specijalnih vrsta piva koja se odlikuju poboljšanom zdravstvenom funkcijom i/ili novim osvežavajućim ukusom se značajno povećalo. Jedna od mogućnosti je i obogaćivanje piva sa bioaktivnim jedinjenjima grožđa koja su odgovorna za dobro poznato blagotvorno dejstvo crvenih vina na zdravlje. U radu je ispitivan uticaj dodatka grožđa sorte Prokupac na fizičkohemijske karakteristike i kinetiku fermentacije specijalnih piva sa dodatkom grožđa, pri čemu su dobijeni rezultati poređeni sa kontrolnim lager pivom. Uticaj dodatka grožđa na aktivnost kvasca je takođe ispitivan. Početni ekstrakt, sadržaj alkohola, stepen prevrelosti, brzina fermentacije i razmnožavanje kvasca je bilo značajno veće kod piva sa dodatkom grožđa, zbog većeg sadržaja prostih šećera u grožđu u poređenju sa čistom sladovinom. Prema CIELab parametrima boje, boja piva sa dodatkom grožđa je bila žuta sa određenim udelom crvene, dok je kontrolno pivo bilo čiste žute boje. Povećanjem udela grožđa boja piva postaje tamnija i sa manjim udelom žute boje, dok je udeo crvene boje direktno proporcionalan sadržaju grožđa. Sadržaj fenolnih jedinjenja i antioksidativni kapacitet piva sa dodatkom grožđa je bio značajno veći u odnosu na kontrolno pivo, što znači da je pivo sa grožđem bolji izvor prirodnih antioksidanasa nego komercijalno lager pivo

ASSOCIATION OF RADIOGRAPHIC FINDINGS OF MUSCULOSKELETAL HAND INVOLVEMENT WITH CLINICAL AND SEROLOGICAL FEATURES OF SYSTEMIC SCLEROSIS

Musculoskeletal manifestations (MSM) frequently occur in systemic sclerosis (SSc) and imply a variety of rheumatic symptoms and clinical features, from arthralgia to arthritis, contractures, tendon friction rubs, tenosynovitis, myalgia, muscle tenderness,  and myositis. The objective of the study was to determine the prevalence of joint manifestations in clinical findings, as well as frequency and type of radiographic changes in 56 patients with the limited and diffuse form of SSc; to define the correlation between musculoskeletal hand changes in SSc and specific antibodies (antinuclear antibodies- ANA), as well as with antibodies specific for particular forms of SSc (anti-topoisomerase-1 antibodies- ATA, anticentromere antibodies- ACA); and to test the correlation between specific cardiopulmonary manifestations in SSc, and frequency and type of musculoskeletal changes. The obtained results indicated a high frequency of joint manifestations in SSc, which were estimated by clinical and radiographic examinations. Joint involvement in SSc was underestimated in clinical trials, as it occurred more frequently than expected. Radiographic hand findings in tested SSc patients indicated the presence of arthritis, erosions, joint space narrowing, radiological demineralization, acro-osteolysis, flexion contractures, and calcinosis. Hand involvement was an important cause of morbidity, which seriously affected the quality of life in patients with SSc. Various forms of joint and bone involvements could represent the base for introducing an innovative approach to treating musculoskeletal hand damage in SSc

PROTECTIVE EFFECT OF QUERCETIN ON CISPLATIN-INDUCED NEPHROTOXICITY IN RATS

The aim of this study was to investigate the possible effect of quercetin on cisplatin-induced nephrotoxicity in rats. Experiments were done on thirty-two Wistar rats divided into four groups of 8 animals each. The CIS group received a single dose of cisplatin (8 mg/kg) intraperitoneally, whereas the CISQ group received quercetin intraperitoneally at a dose of 50 mg/kg for 9 days and a single dose of cisplatin intraperitoneally (8 mg/kg) on the fifth day. Animals in the Q group received quercetin (50 mg/kg) and the C group received saline (1 mL/day), both given intraperitoneally for 9 days. Quantitative evaluation of structural and functional alterations in the kidneys were performed by histopathological and biochemical analyses. Histological sections of kidney in CIS group revealed mild degenerative changes of proximal tubules and focal apoptosis of tubulocites, while glomeruli had reduced lobular appearance. In CISQ group these changes were ameliorated and less visible. Analysis of biochemical parameters showed significantly higher urea and creatinine serum concentrations in CIS group in comparison with C group and CISQ group (p<0.001). The concentrations of potassium and sodium in the CIS group were lower, but not statistically significant in comparison to the C group. Kidney MDA levels were found to be significantly higher in CIS group than those in C group (p<0.001), whereas the values for CISQ group were significantly lower than MDA recorded for CIS group (p<0.001). The results suggest that quercetin has the nephroprotective action and reduces lipid peroxidation in cisplatin-treated rats.Key words: Cisplatin, quercetin, nephrotoxicity, rat

CYTOMORPHOLOGY OF THE BULBAR CONJUNCTIVAL CELLS IN PATIENTS WITH DRY EYE

Dry eye is among the most common pathological conditions in ophthalmology. The aim of our study was to present possibilites of two different cytological methods for examination of cytomorphology of bulbar conjunctival cells- impression cytology (IC) and combined cytological method for scanning electron microscopy in the diagnosis  of dry eye (ICSEM). A hundred and twenty-two patients of both sexes, in different age groups, were analyzed by clinical method (slit lamp, Schirmer I, TBUT, Rose Bengal) and two cytomorphological methods – IC and ICSEM. In patients with dry eye, squamous metaplasia, inflammation and severe loss of adhesiveness of the epithelium were present. ICSEM gives an advantage in early diagnosis of changes, before the lesion of superficial conjunctival epithelium in patients with dry eye. The phenomenon of metaplasia appears in the epithelium of the bulbar conjunctiva in the absence of manifest dry eye and represents the basis for understanding the increased incidence of this syndrome in older patients with dry eye

Primer kombinovanja raspodele atmosferskih aerosola po veličinama dobijenih metodom merenja električne pokretljivosti i optičkom metodom

Atmosferski aerosoli u urbanim sredinama se sastoje od čestica različitih dijametara, koje mogu imati veličinu od par nanometara do par mikrometara. Stoga je za merenje koncentracije aerosola često neophodno koristiti više instrumenata, sa principijelno različitim metodama merenja. U ovom radu su korišćene metode zasnovane na merenju električne pokretljivosti čestica, za opseg dijametara od 10nm do 420nm, i merenju ekvivalentnog optičkog dijametra, za opseg dijametara od 300nm do 10um. Kao glavni rezultat, prikazani su primeri kombinovanja spektara veličina čestica koji su dobijeni pomoću ove dve komplementarne metode merenja. U procesu kombinovanja spektara veličina čestica moguće je modifikovati raspodelu dobijenu optičkim merenjima traženjem optimalne vrednosti indeksa prelamanja čestica tako da se dobije što bolje slaganje sa raspodelom dobijenom merenjem električne pokretljivosti. Kao ulazni podaci su korišćeni rezultati merenja iz kampanje mobilnog monitoringa u Novom Sadu 2022

Applying intellectual capital in improving agri-industry at Republic of Serbia

Analysis of the economic framework for doing business between Serbia and Autonomous Province of Kosovo and Metohija, that is under the interim civil and military administration of the United Nations, according to UN Resolution 1244 (hereinafter referred to as Kosovo*) aims to point of the need to eliminate barriers in doing business between Serbia and Kosovo*, making recommendation for improving the business environment. The research involved experts analyzed legal regulations governing the business environment in Kosovo* and carried out the research. They interviewed business people and representatives of companies from central Serbia and Kosovo*. The results of the survey enable the creation of a wider picture of company operations when crossing of the administrative crossings, identifying disturbances in the passage of goods and services, as well as formulating proposals to overcome existing barriers in doing business between business entities in central Serbia and the Autonomous Province of Kosovo and Metohija