The influence of the high intra-abdominal pressure concerning intra-abdominal hypertension on a mortality rate at the intensive care unit.

Uvod. Pritisak unutar trbušne duplje normalno je nešto iznad atmosferskih vrednosti. Samo malo povećanje intraabdominalnog pritiska može dovesti do niza štetnih posledica u funkciji respiratornog, kardiovaskularnog, bubrežnog, centralnog i gastrointestinalnog sistema. Intraabdominalni pritisak se može meriti direktno invazivnim metodama i indirektno kroz kateter plasiran u mokraćnu bešiku. Indirektni način merenja je jeftin, jednostavan i daje iste rezultate kao i direktno merenje. Znatno povećanje intraabdominalnog pritiska se stalno sreće kod pacijenata u jedinicu intenzivnog lečenja, kod rupture aneurizme abdominalne aorte, abdominalne traume i akutnog pankreatitisa. Abdominalni kompartment sindrom predstavlja kombinaciju povećanog intraabdominalnog pritiska i multiorganske disfunkcije. Ovaj sindrom ima visok stepen smrtnosti, najviše zbog razvoja sepse i multiorganoskog oštećenja organa. Dijagnoza abdominalnog kompartment sindroma zahteva brižljivu identifikaciju pacijenata sa rizikom, redovno merenje intraabdominalnog pritiska, adekvatnu nadoknadu tečnosti i hiruršku dekompresiju. Danas, preporučljiv i ispravan vid lečenja, kod razvijenog abdominalnog kompartment sindroma je hirurška dekompresija. Ciljevi istraživanja. Rano prepoznavanje promena osnovnih vitalnih funkcija kod pacijenata sa povećanim IAP, prognostički faktori vezani za IAP koji utiču na evoluciju patološkog poremećaja, uticaj IAP na stopu smrtosti kod pacijenata suspektnih na IAH Materijal i metod rada. Prospektivna studija je sprovedena kod 100 pacijenta suspektnih na postojanje IAH i ACS u jedinici hirurške intenzivne nege i lečenja u Kliničko bolničkom Centru (KBC) “Zvezdara“ u Beogradu, u periodu januar 2014. do decembra 2015. godine. Rizična grupa pacijenata obuhvatala je pacijente koji imaju faktore rizika za smanjenje komplijanse trbušnog zida (akutno respiratorno oštećenje sa povećanim intratorakalnim pritiskom, abdominalna operacija sa čvrstim fascijalnim zatvaranjem), porast intraluminalnog sadržaja (gastropareza, ileus, pseudo-obstrukcija kolona), porast abdominalnog sadržaja (hemoperitoneum, pneumoperitoneum, ascites, poremećaj funkcije jetre, pankreatitis, peritonitis) i prekomernu nadoknadu tečnosti ili kapilarno krvarenje (acidoza (pH < 7,2), hipotenzija, masivna nadoknada tečnosti (> 5 l /24 sata), oligurija, sepsa). Studija nije obuhvatala pacijente koji su imali traumu, opekotine ili su bili prethodno operisani. Svi pacijenti su podeljeni na osnovu izmerenih vrednosti..

Characterization of Barium Titanate Ceramic Powders by Raman Spectroscopy

Barium titanate, BaTiO3 ceramic powders were prepared by mechanochemical synthesis and by the Pechini method. A powder mixture of BaO and TiO2 was treated in a planetary ball mill in an air atmosphere for Lip to 1 h, using zirconium oxide vial and zirconium oxide balls as the milling medium. After 60 min BaTiO3 phase was formed. In both ways BaTiO3 ceramics were sintered after 2 h on 1300 degrees C without pre-calcinations step. The heating rate was 10 degrees C min(-1). The formation of phase and crystal structure of BaTiO3 was approved by X-ray diffraction analysis and the Raman spectroscopy. The morphology and microstructure of obtained powders were examined by scanning electron microscopy method. Sharp phase transition from ferroelectric to paraelectric state was observed. The hysteresis loop is very well performed with regular sharp characteristic of ferroelectric materials

Development of SARS-CoV-2 N-protein specific capture ELISA

Ta na dijagnoza ljudi sa sumnjom na infekciju SARS-CoV-2 je od suštinskog zna aja za suzbijanje globalnog širenja COVID-19. Prisustvo SARS-CoV-2 može se otkriti RT-PCRom (otkriva RNK virusa) ili detekcijom prisustva virusnih antigena u biološkim te nostima ELISA-om ili sli nom tehnikom koje koriste antitela razvijena u životinjama. Cilj studije je bio uspostavljanje kvantitativnog testa koji se zasniva na koriš enju poliklonskih seruma za rutinsko odre ivanje koncentracije SARS-CoV-2 nukleokapsidnog proteina merenjam apsorbancije u standardnoj mikrotitarskoj plo ici sa 96 bunara. Za potrebe razvoja testa proizveden je rekombinantni N-protein i koriš en za proizvodnju antiseruma u miševima i ze evima. Proizvedeni antiserumi su pre iš eni i odre en im je titar. Poliklonskiantiserumi visokog afiniteta specifi ni za N-protein koriš eni su za razvoj ELISA testa specifi nog za ovaj protein. Test se zasniva na koriš enju poliklonskih seruma miševa koji su adherirani na dno bunara mikrotitarske plo ice za hvatanje N-proteina iz uzorka. Razli ite koncentracije rekombinantnog N-proteina su koriš ene za standardnu krivu za kvantifikaciju proteina. N-protein vezan za antitela miševa je detektovan ze jim poliklonskim serumom i anti-ze jim antitelom povezanim sa enzimom koji obezbe uje spektrofotometrijsko merenje. Uspešno smo razvili prototip ELISA testa za kvantifikaciju N-proteina sa granicom detekcije u opsegu od ng/mL. Prose na vrednost LOD za prototip ELISA testa za detekciju N-proteina je 9,2 ng/mL, dok je prose na vrednost LOQ 10,2 ng/mL. Pokazali smo da su proizvedeni poliklonski antiserumi pogodni za detekciju N-proteina sa sli nim ili boljim afinitetom i specifi noš u od komercijalnih antitela. Štaviše, prototip ELISA testa se može koristiti sa zadovoljavaju om pouzdanoš u za kvantifikaciju N-proteina u uzorcima bogatim proteinima, poput ljudskih seruma.The accurate diagnosis of people with suspected infection with the SARS-CoV-2 is essential to curb the global spread of COVID-19. The presence of SARS-CoV-2 can be detected by RT-PCR (it detects RNA of the virus) or by the presence of viral antigens in biological fluids in ELISA or similar techniques using antibodies developed in animals. The aim of the study was the establishment of a quantitative polyclonal sera-based test for routine measurement of the concentration of SARS CoV-2 nucleocapsid protein using absorbance measurement in a standard 96-well microtiter plate. For the purposes of the test development, recombinant N protein was produced and used for the production of mice and rabbit antisera. Produced antisera were purified and titer was determined. High-affinity polyclonal N-protein specific antisera were used for N-protein specific ELISA test development. The test is based on mice polyclonal sera adhered to microtiter plate bottom for the capture of the N protein from the specimen. Various concentrations of the recombinant N-protein were used to generate a standard curve for protein quantification. The N-protein bound to the mice antibodies was detected with rabbit polyclonal sera and anti-rabbit antibody coupled to an enzyme that provides spectrophotometric measurement. We have successfully developed the prototype ELISA for the quantification of N-protein with the detection limit being in the range of ng/mL. The average LOD value for the prototype ELISA was determined to be 9.2 ng/mL, while the average LOQ value was 10.2 ng/mL. We have demonstrated that produced polyclonal antisera are suitable for the detection of N-protein with affinity and specificity similar to, or better than commercial antibodies. Furthermore, the prototype ELISA can be used with satisfactory confidence for quantification of the N-protein in protein-rich samples, similar to human sera.Poster: [https://cherry.chem.bg.ac.rs/handle/123456789/5362

Development of SARS-CoV-2 N-protein specific capture ELISA

Ta na dijagnoza ljudi sa sumnjom na infekciju SARS-CoV-2 je od suštinskog zna aja zasuzbijanje globalnog širenja COVID-19. Prisustvo SARS-CoV-2 može se otkriti RT-PCRom (otkriva RNK virusa) ili detekcijom prisustva virusnih antigena u biološkim te nostimaELISA-om ili sli nom tehnikom koje koriste antitela razvijena u životinjama. Cilj studijeje bio uspostavljanje kvantitativnog testa koji se zasniva na koriš enju poliklonskih serumaza rutinsko odre ivanje koncentracije SARS-CoV-2 nukleokapsidnog proteina merenjamapsorbancije u standardnoj mikrotitarskoj plo ici sa 96 bunara. Za potrebe razvoja testaproizveden je rekombinantni N-protein i koriš en za proizvodnju antiseruma u miševima ize evima. Proizvedeni antiserumi su pre iš eni i odre en im je titar. Poliklonskiantiserumivisokog afiniteta specifi ni za N-protein koriš eni su za razvoj ELISA testa specifi nog zaovaj protein. Test se zasniva na koriš enju poliklonskih seruma miševa koji su adheriranina dno bunara mikrotitarske plo ice za hvatanje N-proteina iz uzorka. Razli itekoncentracije rekombinantnog N-proteina su koriš ene za standardnu krivu zakvantifikaciju proteina. N-protein vezan za antitela miševa je detektovan ze jimpoliklonskim serumom i anti-ze jim antitelom povezanim sa enzimom koji obezbe ujespektrofotometrijsko merenje. Uspešno smo razvili prototip ELISA testa za kvantifikacijuN-proteina sa granicom detekcije u opsegu od ng/mL. Prose na vrednost LOD za prototipELISA testa za detekciju N-proteina je 9,2 ng/mL, dok je prose na vrednost LOQ10,2 ng/mL. Pokazali smo da su proizvedeni poliklonski antiserumi pogodni za detekcijuN-proteina sa sli nim ili boljim afinitetom i specifi noš u od komercijalnih antitela.Štaviše, prototip ELISA testa se može koristiti sa zadovoljavaju om pouzdanoš u zakvantifikaciju N-proteina u uzorcima bogatim proteinima, poput ljudskih seruma.The accurate diagnosis of people with suspected infection with the SARS-CoV-2 isessential to curb the global spread of COVID-19. The presence of SARS-CoV-2 can bedetected by RT-PCR (it detects RNA of the virus) or by the presence of viral antigens inbiological fluids in ELISA or similar techniques using antibodies developed in animals.The aim of the study was the establishment of a quantitative polyclonal sera-based test forroutine measurement of the concentration of SARS CoV-2 nucleocapsid protein usingabsorbance measurement in a standard 96-well microtiter plate. For the purposes of the testdevelopment, recombinant N protein was produced and used for the production of miceand rabbit antisera. Produced antisera were purified and titer was determined. High-affinitypolyclonal N-protein specific antisera were used for N-protein specific ELISA testdevelopment. The test is based on mice polyclonal sera adhered to microtiter plate bottomfor the capture of the N protein from the specimen. Various concentrations of therecombinant N-protein were used to generate a standard curve for protein quantification.The N-protein bound to the mice antibodies was detected with rabbit polyclonal sera andanti-rabbit antibody coupled to an enzyme that provides spectrophotometric measurement.We have successfully developed the prototype ELISA for the quantification of N-proteinwith the detection limit being in the range of ng/mL. The average LOD value for theprototype ELISA was determined to be 9.2 ng/mL, while the average LOQ value was10.2 ng/mL. We have demonstrated that produced polyclonal antisera are suitable for thedetection of N-protein with affinity and specificity similar to, or better than commercialantibodies. Furthermore, the prototype ELISA can be used with satisfactory confidence forquantification of the N-protein in protein-rich samples, similar to human sera.Abstract: [https://cherry.chem.bg.ac.rs/handle/123456789/5361

The influence of the high intra-abdominal pressure concerning intra-abdominal hypertension on a mortality rate at the intensive care unit.

Uvod. Pritisak unutar trbušne duplje normalno je nešto iznad atmosferskih vrednosti. Samo malo povećanje intraabdominalnog pritiska može dovesti do niza štetnih posledica u funkciji respiratornog, kardiovaskularnog, bubrežnog, centralnog i gastrointestinalnog sistema. Intraabdominalni pritisak se može meriti direktno invazivnim metodama i indirektno kroz kateter plasiran u mokraćnu bešiku. Indirektni način merenja je jeftin, jednostavan i daje iste rezultate kao i direktno merenje. Znatno povećanje intraabdominalnog pritiska se stalno sreće kod pacijenata u jedinicu intenzivnog lečenja, kod rupture aneurizme abdominalne aorte, abdominalne traume i akutnog pankreatitisa. Abdominalni kompartment sindrom predstavlja kombinaciju povećanog intraabdominalnog pritiska i multiorganske disfunkcije. Ovaj sindrom ima visok stepen smrtnosti, najviše zbog razvoja sepse i multiorganoskog oštećenja organa. Dijagnoza abdominalnog kompartment sindroma zahteva brižljivu identifikaciju pacijenata sa rizikom, redovno merenje intraabdominalnog pritiska, adekvatnu nadoknadu tečnosti i hiruršku dekompresiju. Danas, preporučljiv i ispravan vid lečenja, kod razvijenog abdominalnog kompartment sindroma je hirurška dekompresija. Ciljevi istraživanja. Rano prepoznavanje promena osnovnih vitalnih funkcija kod pacijenata sa povećanim IAP, prognostički faktori vezani za IAP koji utiču na evoluciju patološkog poremećaja, uticaj IAP na stopu smrtosti kod pacijenata suspektnih na IAH Materijal i metod rada. Prospektivna studija je sprovedena kod 100 pacijenta suspektnih na postojanje IAH i ACS u jedinici hirurške intenzivne nege i lečenja u Kliničko bolničkom Centru (KBC) “Zvezdara“ u Beogradu, u periodu januar 2014. do decembra 2015. godine. Rizična grupa pacijenata obuhvatala je pacijente koji imaju faktore rizika za smanjenje komplijanse trbušnog zida (akutno respiratorno oštećenje sa povećanim intratorakalnim pritiskom, abdominalna operacija sa čvrstim fascijalnim zatvaranjem), porast intraluminalnog sadržaja (gastropareza, ileus, pseudo-obstrukcija kolona), porast abdominalnog sadržaja (hemoperitoneum, pneumoperitoneum, ascites, poremećaj funkcije jetre, pankreatitis, peritonitis) i prekomernu nadoknadu tečnosti ili kapilarno krvarenje (acidoza (pH < 7,2), hipotenzija, masivna nadoknada tečnosti (> 5 l /24 sata), oligurija, sepsa). Studija nije obuhvatala pacijente koji su imali traumu, opekotine ili su bili prethodno operisani. Svi pacijenti su podeljeni na osnovu izmerenih vrednosti..

Structure study of nanosized La- and Sb-doped BaTiO3

Lanthanum and antimony doped barium titanate powders were prepared by polymeric precursors method through Pechini process. Obtained powder was pressed into pellet and sintering was performed at 1300 degrees C for 8 h. The formation of phase and crystal structure of BaTiO3 was carried out by XRD analysis, Raman and IR spectroscopy. Microstructure properties such as grain size distribution and morphology of sintered samples were determined using scanning electron microscope. Therefore, it was analyzed relation between grain size, structure and properties of obtained ceramics. Influence of La- and Sb-doping on barium titanate properties was discussed. Crow

Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia

Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (D) was 0.688. In combination with resistotyping and gyrA SNP, D increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp(87) - gt Asn in six strains, Asp(87) - gt Gly in one strain, and Ser(83) - gt Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance

Study on bi-layered ceramics powders prepared by the mechanochemical synthesis

Bi-layered structured ferroelectric materials-bismuth titanate, Bi4Ti3O12 (BIT) and barium-bismuth titanate, BaBi4Ti4O15 (BBT) powders were prepared by homogenization and sintering of mixture of stehiometric quantities of bismuth titanate and barium titanate obtained via mechanochemical synthesis. The Bi4Ti3O12 ceramic was obtained at 1000 degrees C for 4 h and BaBi4Ti4O15 ceramic at 1100 degrees C for 4 h. The phase formation and properties of Bi4Ti3O12 and BaBi4Ti4O15 were studied using XRD, Raman spectroscopy, EDS and SEM. Microstructure of Bi4Ti3O12 and BaBi4Ti4O15 exhibits plate-like grains typical for the bi-layered structured material. The Ba2+ addition leads to the change in the microstructure development, particularly in the change of the average grain size. Crow

The spectral analysis of motion: An "open field" activity test example

In this work we have described the new mathematical approach, with spectral analysis of the data to evaluate position and motion in the „„open field““ experiments. The aim of this work is to introduce several new parameters mathematically derived from experimental data by means of spectral analysis, and to quantitatively estimate the quality of the motion. Two original software packages (TRACKER and POSTPROC) were used for transforming a video data to a log file, suitable for further computational analysis, and to perform analysis from the log file. As an example, results obtained from the experiments with Wistar rats in the „open field“ test are included. The test group of animals was treated with diazepam. Our results demonstrate that all the calculated parameters, such as movement variability, acceleration and deceleration, were significantly lower in the test group compared to the control group. We believe that the application of parameters obtained by spectral analysis could be of great significance in assessing the locomotion impairment in any kind of motion. [Projekat Ministarstva nauke Republike Srbije, br. III41007 i br. ON174028

Ion beam modification of TiN thin films - a study of the induced microstructural changes

15th International Conference : IBMM : September 18-22, 2006, Sam Domenico, Taormina, Italy