Learning to Code Through Web Audio: A Team-Based Learning Approach

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.In this article, we discuss the challenges and opportunities provided by teaching programming using web audio technologies and adopting a team-based learning (TBL) approach among a mix of co-located and remote students, mostly novices in programming. The course has been designed for cross-campus teaching and teamwork, in alignment with the two-city master's programme in which it has been delivered. We present the results and findings from: (1) students' feedback; (2) software complexity metrics; (3) students' blog posts; and (4) teacher's reflections. We found that the nature of web audio as a browser-based environment, coupled with the collaborative nature of the course, were suitable for improving the students' level of confidence about their ability in programming. This approach promoted the creation of group course projects of a certain level of complexity, based on the students' interests and programming levels. We discuss the challenges of this approach, such as supporting smooth cross-campus interactions and assuring students' pre-knowledge in web technologies (HTML, CSS, JavaScript) for an optimal experience. We conclude by envisioning the scalability of this course to other distributed and remote learning scenarios in academic and professional settings. This is in line with the foreseen future scenario of cross-site interaction mediated through code

Kif5B and Kifc1 Interact and Are Required for Motility and Fission of Early Endocytic Vesicles in Mouse Liver

Early endocytic vesicles loaded with Texas Red asialoorosomucoid were prepared from mouse liver. These vesicles bound to microtubules in vitro, and upon ATP addition, they moved bidirectionally, frequently undergoing fission into two daughter vesicles. There was no effect of vanadate (inhibitor of dynein) on motility, whereas 5′-adenylylimido-diphosphate (kinesin inhibitor) was highly inhibitory. Studies with specific antibodies confirmed that dynein was not associated with these vesicles and that Kif5B and the minus-end kinesin Kifc1 mediated their plus- and minus-end motility, respectively. More than 90% of vesicles associated with Kifc1 also contained Kif5B, and inhibition of Kifc1 with antibody resulted in enhancement of plus-end–directed motility. There was reduced vesicle fission when either Kifc1 or Kif5B activity was inhibited by antibody, indicating that the opposing forces resulting from activity of both motors are required for fission to occur. Immunoprecipitation of native Kif5B by FLAG antibody after expression of FLAG-Kifc1 in 293T cells indicates that these two motors can interact with each other. Whether they interact directly or through a complex of potential regulatory proteins will need to be clarified in future studies. However, the present study shows that coordinated activity of these kinesins is essential for motility and processing of early endocytic vesicles