An experimental study of transonic flow about a supercritical airfoil

A series of experiments was conducted on flow fields about two airfoil models whose sections are slight modifications of the original Whitcomb supercritical airfoil section. Data obtained include surface static-pressure distributions, far-wake surveys, oil-flow photographs, pitot-pressure surveys in the viscous regions, and holographic interferograms. These data were obtained for different combinations of lift coefficient and free-stream Mach number, which included both subcritical cases and flows with upper-surface shock waves. The availability of both pitot-pressure data and density data from interferograms allowed determination of flow-field properties in the vicinity of the trailing edge and in the wake without recourse to any assumptions about the local static pressure. The data show that significant static-pressure gradients normal to viscous layers exist in this region, and that they persist to approximately 10% chord downstream of the trailing edge. Comparisons are made between measured boundary-layer properties and results from boundary-layer computations that employed measured static-pressure distributions, as well as comparisons between data and results of airfoil flow-field computations

An experimental study of transonic flow about a supercritical airfoil. Static pressure and drag data obtained from tests of a supercritical airfoil and an NACA 0012 airfoil at transonic speeds, supplement

Surface static-pressure and drag data obtained from tests of two slightly modified versions of the original NASA Whitcomb airfoil and a model of the NACA 0012 airfoil section are presented. Data for the supercritical airfoil were obtained for a free-stream Mach number range of 0.5 to 0.9, and a chord Reynolds number range of 2 x 10 to the 6th power to 4 x 10 to the 6th power. The NACA 0012 airfoil was tested at a constant chord Reynolds number of 2 x 10 to the 6th power and a free-stream Mach number range of 0.6 to 0.8

Detailed transonic flow field measurements about a supercritical airfoil section

The transonic flow field about a Whitcomb-type supercritical airfoil profile was measured in detail. In addition to the usual surface pressure distributions and wake surveys, schlieren photographs were taken and velocity vector profiles were determined in the upper surface boundary layer and in the near wake. Spanwise variations in the measured pressures were also determined. The data are analyzed with the aid of an inviscid transonic finite-difference computer program as well as with boundary layer modeling and calculation schemes

Effects of Sting-Support Diameter on the Base Pressures of an Elliptic Cone at Mach Numbers from 0.60 to 1.40

Measurements were made to determine the effects of sting-support diameter on the base pressures of an elliptic cone with ratio of cross-section thickness to width of 1/3 and a plan-form, semi-apex angle of 15 deg. The investigation was made for model angles of attack from -2 deg to +20 deg at Mach numbers from 0.60 to 1.40, and for a constant Reynolds number of 1.4 million, based on the length of the model. The results indicated that the sting interference decreased the base axial-force coefficients by substantial amounts up to a maximum of about one-third the value of the coefficient for no sting interference. There was no practical diameter of the sting for which the effects of the sting on the base pressures would be negligible throughout the Mach number and angle-of-attack ranges of the investigation

Longitudinal Force and Moment Data at Mach Numbers from 0.60 to 1.40 for a Family of Elliptic Cones with Various Semiapex Angles

An investigation has been made to determine the aerodynamic characteristics of four elliptic cones having plan-form semiapex angles ranging from about 9 to 31 deg., and also for one of these cones modified on the upper surface to reduce the base area by about one half. The tests were made for angles of attack from about -2 to +21 deg., at Mach numbers from 0.60 to 1.40, and for a constant Reynolds number of 1.4 million, based on the length of the models. For each model, lift, pitching-moment, and drag coefficients, and lift-drag ratios are presented for the forebody, and axial-force coefficients are presented for the base. Calculated lift and pitching- moment curves for the elliptic cones, and lift-curve slopes for each model at supersonic Mach numbers are shown for comparison with the corresponding experimental values. Lift-drag ratios are also given for the forebody and base combined. These data are presented without discussion

SAMHD1 is a single-stranded nucleic acid binding protein with no active site-associated nuclease activity.

The HIV-1 restriction factor SAMHD1 is a tetrameric enzyme activated by guanine nucleotides with dNTP triphosphate hydrolase activity (dNTPase). In addition to this established activity, there have been a series of conflicting reports as to whether the enzyme also possesses single-stranded DNA and/or RNA 3\u27-5\u27 exonuclease activity. SAMHD1 was purified using three chromatography steps, over which the DNase activity was largely separated from the dNTPase activity, but the RNase activity persisted. Surprisingly, we found that catalytic and nucleotide activator site mutants of SAMHD1 with no dNTPase activity retained the exonuclease activities. Thus, the exonuclease activity cannot be associated with any known dNTP binding site. Monomeric SAMHD1 was found to bind preferentially to single-stranded RNA, while the tetrameric form required for dNTPase action bound weakly. ssRNA binding, but not ssDNA, induces higher-order oligomeric states that are distinct from the tetrameric form that binds dNTPs. We conclude that the trace exonuclease activities detected in SAMHD1 preparations arise from persistent contaminants that co-purify with SAMHD1 and not from the HD active site. An in vivo model is suggested where SAMHD1 alternates between the mutually exclusive functions of ssRNA binding and dNTP hydrolysis depending on dNTP pool levels and the presence of viral ssRNA

Creating and maintaining play connection in a toddler peer group

This study explores how one and two year old peers (henceforth toddlers) participate in joint play activities in a natural group-care setting. We focus on joint play activity between three toddler peers during one full day-care day in a Finnish toddler classroom. Questions guiding the analysis concern the sequential understanding of how play emerges within peer interaction and how toddler peers are able to build sustained co-participation in their joint play during the day. The analysis showed that joint play was fragmented and organized in short segments of dyadic or triadic interaction. Re-establishments of joint play and accumulation of significant play signals during the day were important practices for toddlers to constitute social organization and sustained co-participation in their multi-party peer play. The results strengthen our understanding of very young children as both more and less competent play companions in their peer groups and guide adults’ practice in relation to peer play in toddler classrooms.Peer reviewe

AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation

AID/APOBEC family cytosine deaminases, known to function in diverse cellular processes from antibody diversification to mRNA editing, have also been implicated in DNA demethylation, an important process for transcriptional activation. While oxidation-dependent pathways for demethylation have been described, pathways involving deamination of either 5-methylcytosine (mC) or 5-hydroxymethylcytosine (hmC) have emerged as alternatives. Here, we have addressed the biochemical plausibility of deamination-coupled demethylation. We found that purified AID/APOBECs have substantially reduced activity on mC relative to cytosine, their canonical substrate, and no detectable deamination of hmC. This finding was explained by the reactivity of a series of modified substrates, where steric bulk was increasingly detrimental to deamination. Further, upon AID/APOBEC overexpression, the deamination product of hmC was undetectable in genomic DNA, while oxidation intermediates remained detectable. Our results indicate that the steric requirements for cytosine deamination are one intrinsic barrier to the proposed function of deaminases in DNA demethylation