Methods and Practices of Teaching Online Theatrical Scenic Painting Curricula

This research investigates, at the time of writing, the methods and practices that have proven successful or not for teaching online scenic painting courses. Through the grounding offered by the data collected in this survey, this paper seeks to establish a baseline of common methods and practices currently utilized for scenic painting courses in the online learning environment. The purpose of this work is to offer a strong framework for future online versions of scenic painting curricula. The methodology for the data collection of this work included analyzing published topical documents, the examination and data analysis of results from an anonymous surveying of individuals who have taught these types of courses in this format before as well as drawing from personal experience teaching and learning in online environments

Evaluating the impact of post-translational modifications by the secreted zinc metalloprotease, GelE, on the major autolysin of E. faecalis, AtlA, and a stress-induced protein, SalB

AtlA is the major peptidoglycan hydrolase of E. faecalis involved in cell separation of dividing cells. SalB is a secreted stress-induced protein regulated by the CroRS system. In addition, these two proteins also appear to be affected by the virulence factor, gelatinase (GelE). GelE is a secreted zinc metalloprotease known to impact various cellular functions by post- translational modification of protein substrates. The overall objective of this work was to understand how GelE cleavage of secreted proteins, specifically AtlA and SalB, changes their function. Herein, I discovered that GelE modifies both AtlA and SalB. As visualized by Western blot analysis and flow cytometry, when GelE is expressed, AtlA exists in a N-terminally truncated form. Furthermore, N-terminal-sequencing analysis identified the GelE-cleavage site within AtlA to occur near the catalytic region, Domain II. Thus, cleavage removes the majority of the N-terminal T/E rich region, Domain I. Truncation of AtlA at this site caused no significant difference in the peptidoglycan hydrolysis activity compared to the full-length protein. Nevertheless, the modification of AtlA was shown to be required for cell separation and the completion of cell division. Additionally, GelE-modified AtlA was shown to localize to the cell septum. Taken together, these results demonstrate that post-translational modification of AtlA by GelE regulates AtlA septum localization and successful cell separation. Similarly, in the presence of GelE, SalB was found in multiple fragments. Western blot and flow cytometry analysis demonstrated that SalB was found in the media supernatant, but not associated with the cell surface. Overall, this dissertation demonstrates that GelE post-translationally modifies these two secreted proteins, AtlA and SalB, impacting the function of AtlA in cell division. Future experiments will strengthen our knowledge of how these modifications impact E. faecalis virulence

Borodin: Stalin's Man in China. By DANIEL N. JACOBS. Cambridge, Massachusetts: Harvard University Press, 1981. Pp. 369. Bibliography, index, notes. $25.00.

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Liar, Liar, Pants on Fire: How Companies Misrepresent Their Corporate Social Responsibility

This thesis looks at Corporate Social Responsibility (CSR) in regard to major industries. The goal of this thesis is to answer the question of whether or not companies are adhering to CSR and why. This will be done using an explanation of Ulrich Beck\u27s theory of globalization as well as Karl Marx\u27s classical capitalism theory and neo--Marxian theory. There are three industries examined and each represents an important part of everyday life. Within each industry there was one global or international company selected. They are respectively GlaxoSmithKline (pharmaceuticals), BP (oil and energy) and R.J. Reynolds (tobacco). The research was done by looking at the companies\u27 websites and gathering information on how they presented themselves and then comparing the data with information from news articles. The results of this research showed that across the four dimensions; human rights and ethics, health, education and advertising. There was data that showed that companies were not adhering fully to CSR policies and practices. According to the research human rights and ethics, health and advertising supported Marxian and neo-Marxian theory while education supported Beck\u27s theory of globalization. This research explains how these industries are prompting themselves how they are adhering to their claims and a theoretical explanation of why

Processing of the major autolysin of E. faecalis, AtlA, by the zinc-metalloprotease, GelE, impacts AtlA septal localization and cell separation.

AtlA is the major peptidoglycan hydrolase of Enterococcus faecalis involved in cell division and cellular autolysis. The secreted zinc metalloprotease, gelatinase (GelE), has been identified as an important regulator of cellular function through post-translational modification of protein substrates. AtlA is a known target of GelE, and their interplay has been proposed to regulate AtlA function. To study the protease-mediated post-translational modification of AtlA, monoclonal antibodies were developed as research tools. Flow cytometry and Western blot analysis suggests that in the presence of GelE, surface-bound AtlA exists primarily as a N-terminally truncated form whereas in the absence of GelE, the N-terminal domain of AtlA is retained. We identified the primary GelE cleavage site occurring near the transition between the T/E rich Domain I and catalytic region, Domain II via N-terminal sequencing. Truncation of AtlA had no effect on the peptidoglycan hydrolysis activity of AtlA. However, we observed that N-terminal cleavage was required for efficient AtlA-mediated cell division while unprocessed AtlA was unable to resolve dividing cells into individual units. Furthermore, we observed that the processed AtlA has the propensity to localize to the cell septum on wild-type cells whereas unprocessed AtlA in the ΔgelE strain were dispersed over the cell surface. Combined, these results suggest that AtlA septum localization and subsequent cell separation can be modulated by a single GelE-mediated N-terminal cleavage event, providing new insights into the post-translation modification of AtlA and the mechanisms governing chaining and cell separation

Percentage of cells with four or fewer cells per chain.

<p>Percentage of cells with four or fewer cells per chain.</p