Research Notes: Comparison of subunit compositions and isolectin profiles of the seed lectins purified from Glycine max and G. soja .

The presence of the 120,000 dalton soybean seed lectin (SBL) is controlled by a simple dominant gene designated Le (Orf et al. , 1978). A recent immunological survey of the USDA soybean [Glycinemax (L . ) Merr . ] collection indicated that 2,646 of 2,664 lines are Le (St ahlhut and Hymowitz , 1980), and an analogous study of the USDA G. soja Sieb. & Zucc . collection indicated that 285 of 559 lines contain SBL (Stahlhut et al., 1981 ). SBL preparations from seeds of the soybean lines \u27Beeson\u27, D68-127, \u27 Disoy\u27, \u27Forrest\u27, \u27Harosoy 63\u27, and T-247 apparently are identical; electrophoresis under denaturing conditions separated each lectin into two types of subunits, and isoelectric focusing resolved each into a complex mixture of isolec tins (Su et al. , 1980)

Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257

The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the regulatory gene, nodD1. Expression of nolW is constitutive and independent of flavonoids and nodD1. Normal expression of nolB is retained with a promoter that extends only 61 bp upstream of the transcript start site, but it is lost if an additional 24 bp are removed. Substantial expression of nolW is retained with a promoter that contains only 34 bp of DNA upstream from the transcript initiation site. The dual control region for the two genes is thus only about 109 bp in length.Instituto de Fisiología Vegeta

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