9 research outputs found
The Vehicle, Fall 1992
Table of Contents
DeconstructivismPeter F. Essigpage 5
Homecoming Pep RallyPeter F. Essigpage 6
McAfee GymnasiumWalt Howardpage 7
Morton ParkAnn Moutraypage 9
Why The Willows WeepPeter F. Essigpage 10
UntitledStephen P. Carmodypage 10
A Stranger\u27s MorningBen Hausmannpage 11
deMONSTERative pronounsJoAnna Wolaverpage 12
2.5%Jill S. Pilonpage 13
The BottleStacey Kruegerpage 14
Suppression Jean K. Graypage 15
ProgressStacey Kruegerpage 16
Daily LessonsJennifer Moropage 17
Sunset TheaterMichelle R. Hokepage 20
Eagle GT\u27sJarrod T. Shieldspage 21
New HouseRandy Lisspage 22
UntitledStephen P. Carmodypage 23
Renting Classics on a Saturday NightNancy Jamespage 24
UntitledJacqueline Hallpage 25
Alone While He SleepsSandy Beauchamppage 26
Sand and SeaThomas Schnarrepage 27
loveMichelle R. Hokepage 28
Backward Ass Junkie FunkSandy Beauchamppage 28
These Things You KeepTom McGrathpage 29
Springhill CrestRobert M. Reutherpage 30
The Pass OverLarry Irvinpage 31
The Stolen ChildTom McGrathpage 32
Before the Recycling KickWalt Howardpage 37
Authors\u27 Pagepage 38https://thekeep.eiu.edu/vehicle/1058/thumbnail.jp
Mutational analysis of the C-terminal FATC domain of Saccharomyces cerevisiae Tra1
Tra1 is a component of the Saccharomyces cerevisiae SAGA and NuA4 complexes and a member of the PIKK family, which contain a C-terminal phosphatidylinositol 3-kinase-like (PI3K) domain followed by a 35-residue FATC domain. Single residue changes of L3733A and F3744A, within the FATC domain, resulted in transcriptional changes and phenotypes that were similar but not identical to those caused by mutations in the PI3K domain or deletions of other SAGA or NuA4 components. The distinct nature of the FATC mutations was also apparent from the additive effect of tra1-L3733A with SAGA, NuA4, and tra1 PI3K domain mutations. Tra1-L3733A associates with SAGA and NuA4 components and with the Gal4 activation domain, to the same extent as wild-type Tra1; however, steady-state levels of Tra1-L3733A were reduced. We suggest that decreased stability of Tra1-L3733A accounts for the phenotypes since intragenic suppressors of tra1-L3733A restored Tra1 levels, and reducing wild-type Tra1 led to comparable growth defects. Also supporting a key role for the FATC domain in the structure/function of Tra1, addition of a C-terminal glycine residue resulted in decreased association with Spt7 and Esa1, and loss of cellular viability. These findings demonstrate the regulatory potential of mechanisms targeting the FATC domains of PIKK proteins
Toward Sustainable Environmental Quality: Priority Research Questions for North America.
Anticipating, identifying, and prioritizing strategic needs represent essential activities by research organizations. Decided benefits emerge when these pursuits engage globally important environment and health goals, including the United Nations Sustainable Development Goals. To this end, horizon scanning efforts can facilitate identification of specific research needs to address grand challenges. We report and discuss 40 priority research questions following engagement of scientists and engineers in North America. These timely questions identify the importance of stimulating innovation and developing new methods, tools, and concepts in environmental chemistry and toxicology to improve assessment and management of chemical contaminants and other diverse environmental stressors. Grand challenges to achieving sustainable management of the environment are becoming increasingly complex and structured by global megatrends, which collectively challenge existing sustainable environmental quality efforts. Transdisciplinary, systems-based approaches will be required to define and avoid adverse biological effects across temporal and spatial gradients. Similarly, coordinated research activities among organizations within and among countries are necessary to address the priority research needs reported here. Acquiring answers to these 40 research questions will not be trivial, but doing so promises to advance sustainable environmental quality in the 21st century. Environ Toxicol Chem 2019;38:1606-1624. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC