Local forest structure variability increases resilience to wildfire in dry western U.S. coniferous forests.

A 'resilient' forest endures disturbance and is likely to persist. Resilience to wildfire may arise from feedback between fire behaviour and forest structure in dry forest systems. Frequent fire creates fine-scale variability in forest structure, which may then interrupt fuel continuity and prevent future fires from killing overstorey trees. Testing the generality and scale of this phenomenon is challenging for vast, long-lived forest ecosystems. We quantify forest structural variability and fire severity across >30 years and >1000 wildfires in California's Sierra Nevada. We find that greater variability in forest structure increases resilience by reducing rates of fire-induced tree mortality and that the scale of this effect is local, manifesting at the smallest spatial extent of forest structure tested (90 × 90 m). Resilience of these forests is likely compromised by structural homogenisation from a century of fire suppression, but could be restored with management that increases forest structural variability

Gels under stress: the origins of delayed collapse

Attractive colloidal particles can form a disordered elastic solid or gel when quenched into a two-phase region, if the volume fraction is sufficiently large. When the interactions are comparable to thermal energies the stress-bearing network within the gel restructures over time as individual particle bonds break and reform. Typically, under gravity such weak gels show a prolonged period of either no or very slow settling, followed by a sudden and rapid collapse - a phenomenon known as delayed collapse. The link between local bond breaking events and the macroscopic process of delayed collapse is not well understood. Here we summarize the main features of delayed collapse and discuss the microscopic processes which cause it. We present a plausible model which connects the kinetics of bond breaking to gel collapse and test the model by exploring the effect of an applied external force on the stability of a gel.Comment: Accepted version: 10 pages, 7 figure

Cavity Nest Materials of Northern Flying Squirrels, Glaucomys sabrinus, and North American Red Squirrels, Tamiasciurus hudsonicus, in a Secondary Hardwood Forest of Southern Ontario

Through deployment of artificial nest boxes, we examined the composition of cavity nest materials used by Northern Flying Squirrels (Glaucomys sabrinus) and North American Red Squirrels (Tamiasciurus hudsonicus) in a secondary hardwood forest of southern Ontario, Canada. We collected 32 nests of known species association and found that 85.7% of G. sabrinus nests and 77.8% of T. hudsonicus nests were constructed almost entirely of shredded bark from Eastern White Cedar (Thuja occidentalis). Mean nest depth across all samples was 12.2 cm and showed no significant difference between species or between spring and summer nests. We review the antiparasitic properties of T. occidentalis and suggest that the use of shredded cedar bark by G. sabrinus and T. hudsonicus to line nest cavities may be a behavioural adaptation, which reduces ectoparasite loads in the nest environment

Drone-based Structure-from-Motion provides accurate forest canopy data to assess shading effects in river temperature models

Climatic warming will increase river temperature globally, with consequences for cold water-adapted organisms. In regions with low forest cover, elevated river temperature is often associated with a lack of bankside shading. Consequently, river managers have advocated riparian tree planting as a strategy to reduce temperature extremes. However, the effect of riparian shading on river temperature varies substantially between locations. Process-based models can elucidate the relative importance of woodland and other factors driving river temperature and thus improve understanding of spatial variability of the effect of shading, but characterising the spatial distribution and height of riparian tree cover necessary to parameterise these models remains a significant challenge. Here, we document a novel approach that combines Structure-from-Motion (SfM) photogrammetry acquired from a drone to characterise the riparian canopy with a process based temperature model (Heat Source) to simulate the effects of tree shading on river temperature. Our approach was applied in the Girnock Burn, a tributary of the Aberdeenshire Dee, Scotland. Results show that SfM approximates true canopy elevation with a good degree of accuracy (R2 = 0.96) and reveals notable spatial heterogeneity in shading. When these data were incorporated into a process-based temperature model, it was possible to simulate river temperatures with a similarly-high level of accuracy (RMS

Routes to improving the reliability of low level DNA analysis using real-time PCR

BACKGROUND: Accurate quantification of DNA using quantitative real-time PCR at low levels is increasingly important for clinical, environmental and forensic applications. At low concentration levels (here referring to under 100 target copies) DNA quantification is sensitive to losses during preparation, and suffers from appreciable valid non-detection rates for sampling reasons. This paper reports studies on a real-time quantitative PCR assay targeting a region of the human SRY gene over a concentration range of 0.5 to 1000 target copies. The effects of different sample preparation and calibration methods on quantitative accuracy were investigated. RESULTS: At very low target concentrations of 0.5–10 genome equivalents (g.e.) eliminating any replicates within each DNA standard concentration with no measurable signal (non-detects) compromised calibration. Improved calibration could be achieved by eliminating all calibration replicates for any calibration standard concentration with non-detects ('elimination by sample'). Test samples also showed positive bias if non-detects were removed prior to averaging; less biased results were obtained by converting to concentration, including non-detects as zero concentration, and averaging all values. Tube plastic proved to have a strongly significant effect on DNA quantitation at low levels (p = 1.8 × 10(-4)). At low concentrations (under 10 g.e.), results for assays prepared in standard plastic were reduced by about 50% compared to the low-retention plastic. Preparation solution (carrier DNA or stabiliser) was not found to have a significant effect in this study. Detection probabilities were calculated using logistic regression. Logistic regression over large concentration ranges proved sensitive to non-detected replicate reactions due to amplification failure at high concentrations; the effect could be reduced by regression against log (concentration) or, better, by eliminating invalid responses. CONCLUSION: Use of low-retention plastic tubes is advised for quantification of DNA solutions at levels below 100 g.e. For low-level calibration using linear least squares, it is better to eliminate the entire replicate group for any standard that shows non-detects reasonably attributable to sampling effects than to either eliminate non-detects or to assign arbitrary high Ct values. In calculating concentrations for low-level test samples with non-detects, concentrations should be calculated for each replicate, zero concentration assigned to non-detects, and all resulting concentration values averaged. Logistic regression is a useful method of estimating detection probability at low DNA concentrations

Biodiversidad de insectos acuáticos en Patagonia Norte, Argentina: programa Iniciativa DARWIN

Este proyecto fue financiado durante un período de tres años, desde septiembre de 2006 a través de la Iniciativa Darwin del gobierno Británico. El objetivo de este trabajo es el estudio de los insectos acuáticos del Parque Nacional Nahuel Huapi (PNNHP), Patagonia, Argentina. El parque incluye una gran variedad de hábitats que comprenden arroyos, ríos y lagos los cuales se distribuyen desde el bosque siempreverde frío, hasta la zona árida de estepa. Todo el material será identificado y depositado en las colecciones del Museo La Plata y el Museo de Historia Natural de Londres. Los fondos de la Iniciativa Darwin han sido utilizados para equipar un laboratorio de biodiversidad en las oficinas del PNNHP en Bariloche y la estación de investigación en Puerto Blest. Toda la información de los insectos acuáticos y las distintas formaciones vegetales donde éstos se han encontrado, se incluirá en una base de datos (la primera de su tipo en Patagonia) que utilizará la metodología GIS para analizar patrones de distribución de las especies en el parque. En estos momentos se están produciendo varias guías de campo para identificar los insectos acuáticos del PNNHP. Es nuestro deseo que los resultados obtenidos en este proyecto, contribuyan a la conservación de los sistemas acuáticos en Argentina y América del Sur.This project was funded from September 2006 for three years by the British Government's Darwin Initiative programme. The focus of our project is the study of aquatic insects from Nahuel Huapi National Park (NHNP) in Patagonia, Argentina. The park includes a wide range of wetlands, including montane streams, lowland lakes and marshes, distributed within temperate rainforest and arid steppe. The material will be identified and stored in a fully referenced and accessible collection at La Plata Museum and the Natural History Museum, London. Darwin Initiative funding has been used to equip a biodiversity laboratory at National Park Headquarters in Bariloche and also a field station at Puerto Blest. Information on the insect species in NHNP will be entered into a GIS database, together with a vegetation classification and wetland characteristics, to model freshwater insect data spatially and create a biodiversity database, the first of its kind in Patagonia. We hope that the experiences we gain during the project, the insect collections and databases, the publications, and the many other products, will be used to further enhance wetland conservation throughout Argentina and southern South America.Fil: Brooks, Stephen J.. Natural History Museum; Reino UnidoFil: Hernández, Luis M.. Natural History Museum; Reino UnidoFil: Massaferro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Spinelli, Gustavo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Penn, Malcolm. Natural History Museum; Reino Unid

Biodiversidad de insectos acuáticos en Patagonia Norte, Argentina: programa Iniciativa DARWIN

Este proyecto fue financiado durante un período de tres años, desde septiembre de 2006 a través de la Iniciativa Darwin del gobierno Británico. El objetivo de este trabajo es el estudio de los insectos acuáticos del Parque Nacional Nahuel Huapi (PNNHP), Patagonia, Argentina. El parque incluye una gran variedad de hábitats que comprenden arroyos, ríos y lagos los cuales se distribuyen desde el bosque siempreverde frío, hasta la zona árida de estepa. Todo el material será identificado y depositado en las colecciones del Museo La Plata y el Museo de Historia Natural de Londres. Los fondos de la Iniciativa Darwin han sido utilizados para equipar un laboratorio de biodiversidad en las oficinas del PNNHP en Bariloche y la estación de investigación en Puerto Blest. Toda la información de los insectos acuáticos y las distintas formaciones vegetales donde éstos se han encontrado, se incluirá en una base de datos (la primera de su tipo en Patagonia) que utilizará la metodología GIS para analizar patrones de distribución de las especies en el parque. En estos momentos se están produciendo varias guías de campo para identificar los insectos acuáticos del PNNHP. Es nuestro deseo que los resultados obtenidos en este proyecto, contribuyan a la conservación de los sistemas acuáticos en Argentina y América del Sur.This project was funded from September 2006 for three years by the British Government's Darwin Initiative programme. The focus of our project is the study of aquatic insects from Nahuel Huapi National Park (NHNP) in Patagonia, Argentina. The park includes a wide range of wetlands, including montane streams, lowland lakes and marshes, distributed within temperate rainforest and arid steppe. The material will be identified and stored in a fully referenced and accessible collection at La Plata Museum and the Natural History Museum, London. Darwin Initiative funding has been used to equip a biodiversity laboratory at National Park Headquarters in Bariloche and also a field station at Puerto Blest. Information on the insect species in NHNP will be entered into a GIS database, together with a vegetation classification and wetland characteristics, to model freshwater insect data spatially and create a biodiversity database, the first of its kind in Patagonia. We hope that the experiences we gain during the project, the insect collections and databases, the publications, and the many other products, will be used to further enhance wetland conservation throughout Argentina and southern South America.Facultad de Ciencias Naturales y Muse

The SISO CSPI PDG standard for commercial off-the-shelf simulation package interoperability reference models

For many years discrete-event simulation has been used to analyze production and logistics problems in manufactur-ing and defense. Commercial-off-the-shelf Simulation Packages (CSPs), visual interactive modelling environ-ments such as Arena, Anylogic, Flexsim, Simul8, Witness, etc., support the development, experimentation and visua-lization of simulation models. There have been various attempts to create distributed simulations with these CSPs and their tools, some with the High Level Architecture (HLA). These are complex and it is quite difficult to assess how a set of models/CSP are actually interoperating. As the first in a series of standards aimed at standardizing how the HLA is used to support CSP distributed simula-tions, the Simulation Interoperability Standards Organiza-tion’s (SISO) CSP Interoperability Product Development Group (CSPI PDG) has developed and standardized a set of Interoperability Reference Models (IRM) that are in-tended to clearly identify the interoperability capabilities of CSP distributed simulations

Assessing the potential of drone-based thermal infrared imagery for quantifying river temperature heterogeneity

© 2019 Crown copyright. Hydrological Processes © 2019 John Wiley & Sons, Ltd. Climate change is altering river temperature regimes, modifying the dynamics of temperature-sensitive fishes. The ability to map river temperature is therefore important for understanding the impacts of future warming. Thermal infrared (TIR) remote sensing has proven effective for river temperature mapping, but TIR surveys of rivers remain expensive. Recent drone-based TIR systems present a potential solution to this problem. However, information regarding the utility of these miniaturised systems for surveying rivers is limited. Here, we present the results of several drone-based TIR surveys conducted with a view to understanding their suitability for characterising river temperature heterogeneity. We find that drone-based TIR data are able to clearly reveal the location and extent of discrete thermal inputs to rivers, but thermal imagery suffers from temperature drift-induced bias, which prevents the extraction of accurate temperature data. Statistical analysis of the causes of this drift reveals that drone flight characteristics and environmental conditions at the time of acquisition explain ~66% of the variance in TIR sensor drift. These results shed important light on the factors influencing drone-based TIR data quality and suggest that further technological development is required to enable the extraction of robust river temperature data. Nonetheless, this technology represents a promising approach for augmenting in situ sensor capabilities and improved quantification of advective inputs to rivers at intermediate spatial scales between point measurements and “conventional” airborne or satellite remote sensing

Modelling natural ventilation for summer thermal comfort in Mediterranean dwellings

There is an urgent need for adequate natural ventilation in apartment dwellers in densely built regions that could improve occupants’ summer thermal comfort and reduce the rapidly increasing energy demand for cooling. The aim of the research reported here was to identify natural ventilation strategies that could offer energy efficient refurbishment solutions for domestic buildings whilst maintaining thermal comfort of occupants. An urban multi-storey apartment building in the city of Athens was selected for this study, which is representative of over four-million Greek domestic buildings. Four retrofit strategies were tested with the aim of offering indoor air temperature reductions and increased ventilation rates relative to the existing single-sided ventilation strategy of the building, using computational fluid dynamics (CFD) simulations. The ventilation performance of all strategies varied with wind speed and direction due to the proposed design configurations. During buoyancy driven flows, acceptable ventilation rates for comfort could be achieved with the implementation of a wind-catcher and a dynamic façade. Under wind-driven ventilation, these strategies delivered significant increases in ventilation rate (up to 7 times) and contributed to reductions in indoor air temperatures of up to 2oC, relative to the existing ventilation strategy. Cooling by water evaporation enhanced the cooling performance of the wind-catcher providing up to 4oC temperature reductions. The successful performance of the proposed strategies highlights the potential significance of reducing energy consumption and improving thermal comfort