Working Effectively With Employees who Have Sustained a Brain Injury

This brochure on individuals who have sustained a brain injury and the Americans with Disabilities Act (ADA) is one of a series on human resources practices and workplace accommodations for persons with disabilities edited by Susanne M. Bruyère, Ph.D., CRC, SPHR, Director, Program on Employment and Disability, School of Industrial and Labor Relations – Extension Division, Cornell University. It was updated in June, 2000 by Stephanie Hanson, Ph.D., College of Health Professions, University of Florida. The original as written by Thomas P. Golden, M.S., Program on Employment and Disability, Cornell University. Cornell University was funded in the early 1990’s by the U.S. Department of Education National Institute on Disability and Rehabilitation Research as a National Materials Development Project on the employment provisions (Title I) of the ADA (Grant #H133D10155). These updates, and the development of new brochures, have been funded by Cornell’s Program on Employment and Disability, the Pacific Disability and Business Technical Assistance Center, and other supporters

Development of a novel equine influenza virus live-attenuated vaccine

H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a temperature sensitive (ts) H3N8 EIV live-attenuated influenza vaccine (LAIV) using reverse-genetics approaches. Our EIV LAIV was attenuated (att) in vivo and able to induce, upon a single intranasal administration, protection against H3N8 EIV wild-type (WT) challenge in both a mouse model and the natural host, the horse. Notably, since our EIV LAIV was generated using reverse genetics, the vaccine can be easily updated against drifting or emerging strains of EIV using the safety backbone of our EIV LAIV as master donor virus (MDV). These results demonstrate the feasibility of implementing a novel EIV LAIV approach for the prevention and control of currently circulating H3N8 EIVs in horse populations

Perceptions of Tenure and Promotion Guidelines and Criteria Among Athletic Training Doctoral Students

Purpose: Evidence suggested that athletic training doctoral candidates are being socialized through a variety of formal and informal experiences to prepare them for roles in higher education. It is unclear, however, if they are aware of the expectations associated with tenure and/or promotion which are necessary for transition into higher education. Perceptions of tenure and promotion among doctoral students preparing to enter the athletic training professoriate were investigated. Methods: Twenty-four students (16=females, 8=males, average age=28±3 years) completed one-on-one telephone interviews. Data were analyzed using a general inductive approach, with a peer review and multiple analyst triangulation completed for rigor. Results: Four primary themes emerged from the data: 1) there is a limited understanding of the guidelines and criteria for tenure and promotion, 2) knowledge of guidelines and criteria is perpetuated by mentoring, 3) research/scholarship is perceived as paramount for success, and 4) the belief that skills gained, regardless of the domain of tenure, can facilitate success in others. Conclusions: The findings suggest that doctoral students in athletic training are partially aware of the guidelines for tenure, but could benefit from additional mentoring

Antiferromagnetic Heisenberg Spin Chain of a Few Cold Atoms in a One-Dimensional Trap

We report on the deterministic preparation of antiferromagnetic Heisenberg spin chains consisting of up to four fermionic atoms in a one-dimensional trap. These chains are stabilized by strong repulsive interactions between the two spin components without the need for an external periodic potential. We independently characterize the spin configuration of the chains by measuring the spin orientation of the outermost particle in the trap and by projecting the spatial wave function of one spin component on single-particle trap levels. Our results are in good agreement with a spin-chain model for fermionized particles and with numerically exact diagonalizations of the full few-fermion system

Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells

Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. To better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the effects of Wolbachia in Aedes aegypti mosquito cells and midgut. Perturbations are observed in vesicular trafficking, lipid metabolism and in the endoplasmic reticulum that could impact viral entry and replication. Wolbachia-infected cells display a differential cholesterol profile, including elevated levels of esterified cholesterol, that is consistent with perturbed intracellular cholesterol trafficking. Cyclodextrins have been shown to reverse lipid accumulation defects in cells with disrupted cholesterol homeostasis. Treatment of Wolbachia-infected Ae. aegypti cells with 2-hydroxypropyl-β-cyclodextrin restores dengue replication in Wolbachia-carrying cells, suggesting dengue is inhibited in Wolbachia-infected cells by localised cholesterol accumulation. These results demonstrate parallels between the cellular Wolbachia viral inhibition phenotype and lipid storage genetic disorders

Chemotherapeutic effect of a novel temozolomide analog on nasopharyngeal carcinoma in vitro and in vivo.

BackgroundMany patients with nasopharyngeal carcinoma (NPC) face poor prognosis. Due to its hidden anatomical location, the tumor is usually diagnosed quite late, and despite initially successful treatment with radiation and cisplatin, many patients will relapse and succumb to the disease. New treatment options are urgently needed. We have performed preclinical studies to evaluate the potential NPC therapeutic activity of a newly developed analog of temozolomide (TMZ), an alkylating agent that is the current chemotherapeutic standard of care for patients with malignant glioma.ResultsTMZ was covalently conjugated to the natural monoterpene perillyl alcohol (POH), creating the novel fusion compound NEO212. Its impact on two NPC cell lines was studied through colony formation assays, cell death ELISA, immunoblots, and in vivo testing in tumor-bearing mice. In vitro, NEO212 effectively triggered tumor cell death, and its potency was significantly greater than that of its individual components, TMZ or POH alone. Intriguingly, merely mixing TMZ with POH also was unable to achieve the superior potency of the conjugated compound NEO212. Treatment of NPC cells with NEO212 inactivated the chemoprotective DNA repair protein MGMT (O6-methylguanine methyltransferase), resulting in significant chemosensitization of cells to a second round of drug treatment. When tested in vivo, NEO212 reduced tumor growth in treated animals.ConclusionOur results demonstrate anticancer activity of NEO212 in preclinical NPC models, suggesting that this novel compound should be evaluated further for the treatment of patients with NPC

A mus-51 RIP allele for transformation of Neurospora crassa

This report describes the construction and characterization of mus-51RIP70, an allele for high-efficiency targeted integration of transgenes into the genome of the model eukaryote Neurospora crassa. Two of the mus-51RIP70 strains investigated in this work (RZS27.10 and RZS27.18) can be obtained from the Fungal Genetics Stock Center. The two deposited strains are, to our knowledge, genetically identical and neither one is preferred over the other for use in Neurospora research

Factors of Stress, Burnout, and Frustrations Experienced by Athletic Training Students

Purpose: College students are at a risk for increased levels of stress and burnout, and those enrolled in healthcare degree programs are more likely to experience higher levels due to balancing demanding roles. Our purpose was to assess sources of stress, burnout, and/or frustration in athletic training students (ATSs) of different academic standings. Methods: Data were collected through the use of an original, 33-question questionnaire. An inductive approach was used to analyze the data and multiple analyst triangulation and interpretive member checks were used to secure credibility. Results: 27 male and female ATSs from freshman, sophomore, junior, and senior classes from one institution voluntarily participated within this study. We were able to identify distinct themes of stress and burnout for each class of ATSs. Freshman reported concern for the time demand from the major and a desire to have more hands-on activity involved with their clinical education experiences. The sophomores reported feeling stressed regarding the expectation to retain information and the expectations to perform hands-on duties, as well as their ability to balance academic and clinical responsibilities. Challenging coursework, pressure during clinical experiences, and social conflicts appeared as themes for the junior ATSs. Finally, the seniors stated a fear of not achieving professional proficiency was a source of frustration. Conclusions and Recommendations: Based on our findings, efforts should be made to assist student time management practices, improve communication between preceptors and students, and make expectations of the athletic training program (ATP) faculty and preceptors clear. Such measures could help influence other positive changes within ATPs to improve the learning environment for ATSs