Rotating mandrel for assembly of inflatable devices Patent

Rotating, multisided mandrel for fabricating gored inflatable spacecraf

Sequential and ordered assembly of E1 initiator complexes on the papillomavirus origin of DNA replication generates progressive structural changes related to melting

Multiple binding sites for an initiator protein are a common feature of replicator sequences from various organisms. By binding to the replicator, initiators mark the site and contribute to melting or distortion of the DNA by largely unknown mechanisms. Here we analyze origin of DNA replication (ori) binding by the E1 initiator and show sequential binding to a set of overlapping binding sites. The assembly of these initiator complexes is controlled by a gradual reduction in the dependence of interactions between the initiator and DNA and a gradual increase in the reliance on interactions between initiator molecules, providing a mechanism for sequential and orderly assembly. Importantly, the binding of the initiator causes progressive structural alterations both in the sites and in the sequences flanking the sites, eventually generating severe structural alterations. These results indicate that the process of template melting may be incremental, where binding of each initiator molecule serves as a wedge that upon binding gradually alters the template structure. This mechanism may explain the requirement for multiple initiator binding sites that is observed in many ori's

Traveling sealer for contoured table Patent

Sealing apparatus for joining two pieces of frangible material

Transcription factor-dependent loading of the E1 initiator reveals modular assembly of the papillomavirus origin melting complex

Replication of bovine papillomavirus type 1 DNA absolutely requires the viral transcription factor E2 as well as the initiator Fl, although Fl alone has all the activities expected of an initiator protein. El assembles on the DNA in a stepwise fashion and undergoes a transition in activities from site-specific DNA-binding protein to mobile helicase. Complex assembly is assisted by the viral transcription factor E2 at two levels. E2 acts generally as a specificity factor, which through cooperative binding with El generates an initial Fl complex containing three Fl dimers bound to ori on one face of the DNA, El-ori. Furthermore, E2 can promote the transition to an ori melting complex by recruiting additional Fl molecules to ori, effectively reducing the Fl concentration required for ori melting. This reaction is dependent on an Ea-binding site positioned distal to the precursor E1-ori complex. The final origin melting complex has two subunits that each encircle the DNA and function independently to melt ori. The assembly pathway we describe has implication for understanding DNA melting and unwinding reactions, which are generally poorly understood

Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity

We have analyzed two residues in the helicase domain of the E1 initiator protein. These residues are part of a highly conserved structural motif, the beta-hairpin, which is present in the helicase domain of all papovavirus initiator proteins. These proteins are unique in their ability to transition from local template melting activity to unwinding. We demonstrate that the beta-hairpin has two functions. First, it is the tool used by the E1 double trimer (DT) to pry open and melt double-stranded DNA. Second, it is required for the unwinding activity of the hexameric E1 helicase. The fact that the same structural element, but not the same residues, contacts both dsDNA in the DT for melting and ssDNA in the double hexamer (DH) for helicase activity provides a link between local origin melting and DNA helicase activity and suggests how the transition between these two states comes about

Identified Particle Jet Correlations from PHENIX

Two-particle azimuthal correlations have been shown to be a powerful probe for extracting novel features of the interaction between hard scattered partons and the medium produced in Au+Au collisions at RHIC. At intermediate $p_T$, 2-5GeV/c, jets have been shown to be significantly modified in both particle composition and angular distribution compared to p+pcollisions. We present recent PHENIX results from Au+Au collisions for a variety of $p_T$ and particle combinations.Comment: Parallel talk given at Quark Matter 2006, Shanghai Chin

Deterministic Walks in Quenched Random Environments of Chaotic Maps

This paper concerns the propagation of particles through a quenched random medium. In the one- and two-dimensional models considered, the local dynamics is given by expanding circle maps and hyperbolic toral automorphisms, respectively. The particle motion in both models is chaotic and found to fluctuate about a linear drift. In the proper scaling limit, the cumulative distribution function of the fluctuations converges to a Gaussian one with system dependent variance while the density function shows no convergence to any function. We have verified our analytical results using extreme precision numerical computations.Comment: 18 pages, 9 figure

Rotating mandrel speeds assembly of plastic inflatables

Rotating mandrel permits the accurate cutting, forming, and sealing of plastic gores for assembly of an inflatable surface of revolution. The gores remain on the mandrel until the final seam is reached. Tolerances are tightly controlled by the mandrel configuration

Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genome

The DNA context of nucleotides that a protein recognizes can influence the strength of the protein-DNA interaction. Moreover, in prokaryotes, understanding the quantitative differences in binding affinities that result in part from the DNA context is often important in describing regulatory mechanisms. Nevertheless, these issues have not been a major focus yet for the investigation of protein-DNA interactions in eukaryotes. In this study, we explored the binding specificity and the range of affinities that the BPV-1 E2 transcriptional activator has for DNA. Because E2 binding sites are positioned near several different BPV-1 promoters, such quantitative information may be important to understand transcriptional regulatory mechanisms in BPV-1. Gel retardation assays and DNA footprinting were used to quantitate the affinities of the E2 binding sites in the viral genome. In the process, five sites were discovered, which, on the basis of sequence, had not been predicted previously to interact with the E2 protein. Equilibrium and kinetic studies show that the range of E2 affinities of the 17 sites varied over 300-fold. The sequence elements responsible for E2 recognition of DNA were determined by missing contact analysis of several sites and a point mutation analysis of one site. The results presented show that the affinity of an E2 binding site is to a large extent determined by the availability of specific contacts, but the data also strongly suggest that DNA structure plays an important role

Peculiarities in produced particles emission in 208Pb + Ag(Br) interactions at 158 A GeV/c

The angular structures of particles produced in 208Pb induced collisions with Ag(Br) nuclei in an emulsion detector at 158 A GeV/c have been investigated. Nonstatistical ring-like substructures in azimuthal plane of the collision have been found and their parameters have been determined. The indication on the formation of the ring-like substructures from two symmetrical emission cones - one in the forward and other in the backward direction in the center-of mass system have been obtained. The ring-like substructures parameters have been determined. The experimental results are in an agreement with I.M. Dremin idea, that mechanism of the ring-like substructures formation in nuclear collisions is similar to that of Cherenkov electromagnetic radiation.Comment: 10 pages, 7 figures, Report at the HADRON STRUCTURE'04 Conference, Smolenice, Slovakia, 30.8.-3.9.200