Release of rosmarinic acid from semisolid formulations, and its penetration through the human skin ex vivo

The aim of this study was to evaluate the release of rosmarinic acid (RA) from the experimental topical formulations with the Melissa officinalis L. extract and to evaluate its penetration through undamaged human skin ex vivo. The results of the in vitro release study showed that higher amounts of RA were released from the emulsion vehicle when lemon balm extract was added in its dry form. An inverse correlation was detected between the released amount of RA and the consistency index of the formulation. Different penetration of RA into the skin may be influenced by the characteristics of the vehicle as well as by the form of the extract. The results of penetration assessment showed that the intensity of RA penetration was influenced by its lipophilic properties: RA was accumulating in the epidermis, while the dermis served as a barrier, impeding its deeper penetration

Examination of rheological and physicochemical characteristics in Lithuanian honey

The aim of the study was to determine the effect of the biological origin and temperature on rheological and physicochemical characteristics of honey. The honey of the selected varieties differed in color, scent, microstructure, total acidity, moisture, and pH. The level of moisture statistically significantly correlated with the rheological characteristics. The highest total acidity was a characteristic of forest and buckwheat honey, and the lowest of acacia honey. The lowest pH value (3.76) was found in forest and rape honey, and the highest in eucalyptus honey. Moisture levels significantly correlated with the rheological characteristics. All varieties of honey at all the studied temperatures were characterized by properties of non-Newtonian substances. The study showed that the effect of temperature on rheological characteristics of different types of honey varied and was more pronounced in honey with a crystalline structure, and less pronounced in non-crystalline honey.Key words: Honey, rheological characteristics, physicochemical properties

MoModeling, rheological and biopharmaceutical evaluation of semisolid formulations with natural substances

[...]. The aim of this work was to model stable, evidenced by research semisolid formulations with natural components and to evaluate its rheological and biopharmaceutical properties. The objectives of work were: 1. To model semisolid bases using natural substances and evaluate its rheological characteristics and stability. 2. To evaluate possibilities to use honey in modelling of semisolid formulations. 3. To evaluate antiradical and antimicrobial activity and penetration into human skin possibilities of components of hawthorn (chlorogenic acid, vitexin-2"-O-rhamnoside, hyperoside, isoquercitrin) and lemon balm (rosmarinic acid) extracts with in vitro and ex vivo methods. 4. To investigate selected herbal extracts influence to the rheological properties of semisolid formulations. 5. To determine release and penetration kinetics of the main phenolic components of selected herbal extracts from the semisolid formulations with the biopharmaceutical methods in vitro. [...]

Anatomical and chemical properties of Allium ursinum L. and protection in Lithuania

Straipsnyje pateikiama meškinio česnako (Allium ursinum L. ) česnakinių (Alliaceae J. Agrdh.) šeimos daugiamečio žolinio, vaistinio, maistinio, reto augalo anatominės, cheminės savybės bei paplitimas ir sauga Lietuvoje. Iš bandinių viršerdvės, naudojant kietafazę mikroekstrakciją, atlikta lakių junginių sorbcija. Lakių junginių sudėtis tirta dujų chromatografu su masių spektrometriniu detektoriumi.[...]Ramsons (Allium ursinum L., f. Alliaceae J. Agrdh) is rare, perennial, graminaceous, nutritious, medicinal plant, which anatomical and chemical characteristics, spreading and protection in Lithuania are discussed in this article. Solid phase microextraction and gas-chromatographic analysis of Allium ursinum L. samples was carried out. The results obtained indicate, that composition of volatile compounds is highly dependent on the conditions of the sample preparation, storage conditions and on the plant part investigated.[…]Biochemijos katedraBotanikos sodasKauno medicinos universitetasVytauto Didžiojo universitetasŠvietimo akademij

Phytochemical analysis and classification according to growth sites in Lithuania of Chamerion angustifolium L. using chromatographic and related techniques

Biochemijos katedraBotanikos sodasGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Phytochemical analysis of Chamerion angustifolium L. by means of gas chromatography-mass spectrometry

Biochemijos katedraBotanikos sodasGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

High performance liquid chromatography analysis and radical scavenging evaluation of Chamerion angustifolium L. differently prepared extracts

Biochemijos katedraBotanikos sodasGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Analysis of antiproliferative effect of Chamerion angustifolium water extract and its fractions on several breast cancer cell lines

Original research article ; authors (15) ; the Study was financially supported by the Research Council of Lithuania (grant no. MIP 084/2012 ONKOFITAS)PURPOSE: To evaluate the antiproliferative effect of the aerial part of Chamerion angustifolium (L.) Holub. (Onagraceae) extract and its fractions in vitro. This is the first study on the anti-proliferative effect of C. angustifolium on 3 distinct breast cancer cell lines. MATERIAL/METHODS: Breast cancer cell lines MCF7, MDA-MB-468 and MDA-MB-231 were exposed to different concentrations of the water extract of C. angustifolium, where DPPH radical scavenging activity was 0.018-0.443mg/ml, expressed in rutin equivalents. Cell growth was analyzed after 24, 48 and 72h of incubation. Solid-phase extraction was applied for the fractionation of C. angustifolium water extract and MDA-MB-468 cell line growth was tested using different fractions. RESULTS: The concentrations corresponding to radical scavenging activity of 0.117 and 0.266mg/ml caused MCF7 cells growth inhibition, while in the samples exposed to the highest concentration (0.355 and 0.443mg/ml) no proliferation was register, suggesting cell death. MDA-MB-468 cell analysis showed similar responses. MDA-MB-231 demonstrated cell growth inhibition following the exposure to all analyzed high extract doses (0.117-0.443mg/ml). MDA-MB-468 cells were selected to evaluate the effect of fractions. In the samples exposed to the fraction containing the highest amount (91%) of oenothein B, at the concentration of 0.117mg/ml a pronounced cell growth inhibition while at higher concentrations (0.266 and 0.443mg/ml) no cell proliferation was observed. CONCLUSIONS: The consumption of C. angustifolium herb can be advantageous, alongside with conventional breast cancer treatmentBiochemijos katedraBotanikos sodasInstrumentinės analizės APCLietuvos sveikatos mokslų universitetas. Medicinos akademijaLietuvos sveikatos mokslų universitetas. Medicinos akademija. Onkologijos institutasVytauto Didžiojo universiteta