402 research outputs found
S-FaaS: Trustworthy and Accountable Function-as-a-Service using Intel SGX
Function-as-a-Service (FaaS) is a recent and already very popular paradigm in
cloud computing. The function provider need only specify the function to be
run, usually in a high-level language like JavaScript, and the service provider
orchestrates all the necessary infrastructure and software stacks. The function
provider is only billed for the actual computational resources used by the
function invocation. Compared to previous cloud paradigms, FaaS requires
significantly more fine-grained resource measurement mechanisms, e.g. to
measure compute time and memory usage of a single function invocation with
sub-second accuracy. Thanks to the short duration and stateless nature of
functions, and the availability of multiple open-source frameworks, FaaS
enables non-traditional service providers e.g. individuals or data centers with
spare capacity. However, this exacerbates the challenge of ensuring that
resource consumption is measured accurately and reported reliably. It also
raises the issues of ensuring computation is done correctly and minimizing the
amount of information leaked to service providers.
To address these challenges, we introduce S-FaaS, the first architecture and
implementation of FaaS to provide strong security and accountability guarantees
backed by Intel SGX. To match the dynamic event-driven nature of FaaS, our
design introduces a new key distribution enclave and a novel transitive
attestation protocol. A core contribution of S-FaaS is our set of resource
measurement mechanisms that securely measure compute time inside an enclave,
and actual memory allocations. We have integrated S-FaaS into the popular
OpenWhisk FaaS framework. We evaluate the security of our architecture, the
accuracy of our resource measurement mechanisms, and the performance of our
implementation, showing that our resource measurement mechanisms add less than
6.3% latency on standardized benchmarks
Detection of nanoplastic by surface-enhanced Raman microscopy
Currently the extent of nanoplastic in the environment can only be estimated
by extrapolation from the plastic waste that can be detected. To be able to
quantify the whole extent of the problem, detection methods have to be
developed that can also identify particles that are smaller than 1 m. Here
we employ surface-enhanced Raman scattering (SERS) to image and identify single
nanoplastic particles down to 100 nm in size. We obtain an experimental
enhancement factor of more than three orders of magnitude measured on a single
plastic particle instead of averaging over a concentration. Our results
contribute to the better understanding and employment of SERS for nanoparticle
detection and present an important step for the development of future sensors.Comment: 9 pages, 7 figure
Severe ovarian hyperstimulation syndrome after letrozole-gonadotropin stimulation: a case report
The curse of experiencing and committing violence as a criminal recidivism predictor: a comparison between female forensic psychiatric patients with severe mental disorders and substance use disorder
The fecal microbiome and metabolome differs between dogs fed Bones and Raw Food (BARF) diets and dogs fed commercial diets
Introduction: Feeding a Bones and Raw Food (BARF) diet has become an increasing trend in canine nutrition. Bones and Raw Food diets contain a high amount of animal components like meat, offal, and raw meaty bones, combined with comparatively small amounts of plant ingredients like vegetables and fruits as well as different sorts of oil and supplements. While many studies have focused on transmission of pathogens via contaminated meat and on nutritional imbalances, only few studies have evaluated the effect of BARF diets on the fecal microbiome and metabolome. The aim of the study was to investigate differences in the fecal microbiome and the metabolome between dogs on a BARF diet and dogs on a commercial diet (canned and dry dog food).
Methods: Naturally passed fecal samples were obtained from 27 BARF and 19 commercially fed dogs. Differences in crude protein, fat, fiber, and NFE (Nitrogen-Free Extract) between diets were calculated with a scientific nutrient database. The fecal microbiota was analyzed by 16S rRNA gene sequencing and quantitative PCR assays. The fecal metabolome was analyzed in 10 BARF and 9 commercially fed dogs via untargeted metabolomics approach.
Results: Dogs in the BARF group were fed a significantly higher amount of protein and fat and significantly lower amount of NFE and fiber. There was no significant difference in alpha-diversity measures between diet groups. Analysis of similarity (ANOSIM) revealed a significant difference in beta-diversity (p < 0.01) between both groups. Linear discriminant analysis effect size (LefSe) showed a higher abundance of Lactobacillales, Enterobacteriaceae, Fusobacterium and, Clostridium in the BARF group while conventionally fed dogs had a higher abundance of Clostridiaceae, Erysipelotrichaceae, Ruminococcaceae, and Lachnospiraceae. The qPCR assays revealed significantly higher abundance of Escherichia coli (E. coli) and Clostridium (C.). perfringens and an increased Dysbiosis Index in the BARF group. Principal component analysis (PCA) plots of metabolomics data showed clustering between diet groups. Random forest analysis showed differences in the abundance of various components, including increased 4-hydroxybutryric acid (GBH) and 4-aminobutyric acid (GABA) in the BARF group. Based on univariate statistics, several metabolites were significantly different between diet groups, but lost significance after adjusting for multiple comparison. No differences were found in fecal bile acid concentrations, but the BARF group had a higher fecal concentration of cholesterol in their feces compared to conventionally fed dogs.
Conclusion: Microbial communities and metabolome vary significantly between BARF and commercially fed dogs
Combining targeted and systematic prostate biopsy improves prostate cancer detection and correlation with the whole mount histopathology in biopsy naïve and previous negative biopsy patients
OBJECTIVE: Guidelines for previous negative biopsy (PNB) cohorts with a suspicion of prostate cancer (PCa) after positive multiparametric (mp) magnetic-resonance-imaging (MRI) often favour the fusion-guided targeted prostate-biopsy (TB) only approach for Prostate Imaging-Reporting and Data System (PI-RADS) ≥3 lesions. However, recommendations lack direct biopsy performance comparison within biopsy naïve (BN) vs. PNB patients and its prognostication of the whole mount pathology report (WMPR), respectively. We suppose, that the combination of TB and concomitant TRUS-systematic biopsy (SB) improves the PCa detection rate of PI-RADS 2, 3, 4 or 5 lesions and the International Society of Urological Pathology (ISUP)-grade predictability of the WMPR in BN- and PNB patients. METHODS: Patients with suspicious mpMRI, elevated prostate-specific-antigen and/or abnormal digital rectal examination were included. All PI-RADS reports were intramurally reviewed for biopsy planning. We compared the PI-RADS score substratified TB, SB or combined approach (TB/SB) associated BN- and PNB-PCa detection rate. Furthermore, we assessed the ISUP-grade variability between biopsy cores and the WMPR. RESULTS: According to BN (n = 499) vs. PNB (n = 314) patients, clinically significant (cs) PCa was detected more frequently by the TB/SB approach (62 vs. 43%) than with the TB (54 vs. 34%) or SB (57 vs. 34%) (all p < 0.0001) alone. Furthermore, we observed that the TB/SB strategy detects a significantly higher number of csPCa within PI-RADS 3, 4 or 5 reports, both in BN and PNB men. In contrast, applied biopsy techniques were equally effective to detect csPCa within PI-RADS 2 lesions. In case of csPCa diagnosis the TB approach was more often false-negative in PNB patients (BN 11% vs. PNB 19%; p = 0.02). The TB/SB technique showed in general significantly less upgrading, whereas a higher agreement was only observed for the total and BN patient cohort. CONCLUSION: Despite csPCa is more frequently found in BN patients, the TB/SB method always detected a significantly higher number of csPCa within PI-RADS 3, 4 or 5 reports of our BN and PNB group. The TB/SB strategy predicts the ISUP-grade best in the total and BN cohort and in general shows the lowest upgrading rates, emphasizing its value not only in BN but also PNB patients
The Effects of Anthrax Lethal Toxin on Host Barrier Function
The pathological actions of anthrax toxin require the activities of its edema factor (EF) and lethal factor (LF) enzyme components, which gain intracellular access via its receptor-binding component, protective antigen (PA). LF is a metalloproteinase with specificity for selected mitogen-activated protein kinase kinases (MKKs), but its activity is not directly lethal to many types of primary and transformed cells in vitro. Nevertheless, in vivo treatment of several animal species with the combination of LF and PA (termed lethal toxin or LT) leads to morbidity and mortality, suggesting that LT-dependent toxicity is mediated by cellular interactions between host cells. Decades of research have revealed that a central hallmark of this toxicity is the disruption of key cellular barriers required to maintain homeostasis. This review will focus on the current understanding of the effects of LT on barrier function, highlighting recent progress in establishing the molecular mechanisms underlying these effects
Resurrection of Anopheles goeldii from synonymy with Anopheles nuneztovari (Diptera, Culicidae) and a new record for Anopheles dunhami in the Brazilian Amazon
- …