508 research outputs found
Letter, 1910, July, Pauline Steinem to Dear Friend [Martha McClellan Brown]
A letter from Pauline Steinem, President of the Ohio Woman Suffrage Association asking for an extra effort on behalf of every club...to make the [state] convention a success.https://corescholar.libraries.wright.edu/special_ms147_correspondence/1011/thumbnail.jp
Gloria Steinem Keynote Address
In March of 2008, the University of Baltimore School of Law hosted its first annual Feminist Legal Theory Conference. Gloria Steinem was invited to share her wisdom. Here is how she concluded our event
Gloria Steinem Keynote Address
In March of 2008, the University of Baltimore School of Law hosted its first annual Feminist Legal Theory Conference. Gloria Steinem was invited to share her wisdom. Here is how she concluded our event
Fully-Automatic Multiresolution Idealization for Filtered Ion Channel Recordings: Flickering Event Detection
We propose a new model-free segmentation method, JULES, which combines recent
statistical multiresolution techniques with local deconvolution for
idealization of ion channel recordings. The multiresolution criterion takes
into account scales down to the sampling rate enabling the detection of
flickering events, i.e., events on small temporal scales, even below the filter
frequency. For such small scales the deconvolution step allows for a precise
determination of dwell times and, in particular, of amplitude levels, a task
which is not possible with common thresholding methods. This is confirmed
theoretically and in a comprehensive simulation study. In addition, JULES can
be applied as a preprocessing method for a refined hidden Markov analysis. Our
new methodolodgy allows us to show that gramicidin A flickering events have the
same amplitude as the slow gating events. JULES is available as an R function
jules in the package clampSeg
Heterogeneous Idealization of Ion Channel Recordings -- Open Channel Noise
We propose a new model-free segmentation method for idealizing ion channel
recordings. This method is designed to deal with heterogeneity of measurement
errors. This in particular applies to open channel noise which, in general, is
particularly difficult to cope with for model-free approaches. Our methodology
is able to deal with lowpass filtered data which provides a further
computational challenge. To this end we propose a multiresolution testing
approach, combined with local deconvolution to resolve the lowpass filter.
Simulations and statistical theory confirm that the proposed idealization
recovers the underlying signal very accurately at presence of heterogeneous
noise, even when events are shorter than the filter length. The method is
compared to existing approaches in computer experiments and on real data. We
find that it is the only one which allows to identify openings of the PorB
porine at two different temporal scales. An implementation is available as an R
package
Single Hemichannels Recorded in Lipid Bilayers and Artificial Gap Junction Formation with Cells
Feminist Scholarship Review: Intolerance and Humanism
Published from 1991 through 2007 at Trinity College, Hartford, Connecticut, the Feminist Scholarship Review is a literary journal that describes women\u27s experiences around the world. FSR began as a review of feminist scholarly material, but evolved into a journal for poetry and short storie
Voltage-dependent structural changes of the membrane-bound anion channel hVDAC1 probed by SEIRA and electrochemical impedance spectroscopy
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.The voltage-dependent anion channel (VDAC) is a transmembrane protein that regulates the transfer of metabolites between the cytosol and the mitochondrium. Opening and partial closing of the channel is known to be driven by the transmembrane potential viaa mechanism that is not fully understood. In this work, we employed a spectroelectrochemical approach to probe the voltage-induced molecular structure changes of human VDAC1 (hVDAC1) embedded in a tethered bilayer lipid membrane on a nanostructured Au electrode. The model membrane consisted of a mixed self-assembled monolayer of 6-mercaptohexanol and (cholesterylpolyethylenoxy)thiol, followed by the deposition of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine vesicles including hVDAC1. The stepwise assembly of the model membrane and the incorporation of hVDAC1 were monitored by surface enhanced infrared absorption and electrochemical impedance spectroscopy. Difference spectra allowed for identifying the spectral changes which may be associated with the transition from the open to the “closed” states by shifting the potential above or below the transmembrane potential determined to beca.0.0 Vvs.the open circuit potential. These spectral changes were interpreted on the basis of the orientation- and distance-dependent IR enhancement and indicate alterations of the inclination angle of the β-strands as crucial molecular events, reflecting an expansion or contraction of the β-barrel pore. These protein structural changes that do not confirm nor exclude the reorientation of the α-helix are either directly induced by the electric field or a consequence of a potential-dependent repulsion or attraction of the bilayer.DFG, EXC 314, Unifying Concepts in CatalysisDFG, SFB 803, Funktionalität kontrolliert durch Organisation in und zwischen Membrane
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