Interchange instability in an accretion disc with a poloidal magnetic field

We investigate the stability to nonaxisymmetric perturbations of an accretion disc in which a poloidal magnetic field provides part of the radial support against gravity. Interchange instability due to radial gradients in the magnetic field are strongly stabilized by the shear flow in the disc. For smooth field distributions this instability is restricted to discs in which the magnetic energy is comparable to the gravitational energy. An incompressible model for the instability akin to the Boussinesq approximation for convection is given which predicts the behaviour of the instability accurately. Global axisymmetric disturbances are also considered and found to be stable for a certain class of models. The results indicate that accretion discs may be able to support poloidal fields which are strong enough to suppress other forms of magnetic instability. These strong and stable field distributions are likely to be well suited for the magnetic acceleration of jets and winds.Comment: uuencoded gzip'ed postscript, 9 page

Curved Herbig-Haro Jets: Simulations and Experiments

Herbig-Haro jets often show some degree of curvature along their path, in many cases produced by the ram pressure of a side-wind. We present simulations of both laboratory and astrophysical curved jets and experimental results from laboratory experiments. We discuss the properties and similarities of the laboratory and astrophysical flow, which show the formation of internal shocks and working surfaces. In particular the results illustrate how the break-up of the bow-shock and clumps in the flow are produced without invoking jet variability; we also discuss how jet rotation reduces the growth of the Rayleigh-Taylor instability in curved jets.Comment: 15 pages, 5 figure, accepted to be published in The Astrophysical Journa

A mystery solved: the mass ratio of the dwarf nova EM Cygni

We have discovered that the spectrum of the well-known dwarf nova EM Cyg is contaminated by light from a K2-5V star (in addition to the K-type mass donor star). The K2-5V star contributes approximately 16 per cent of the light from the system and if not taken into account has a considerable effect upon radial velocity measurements of the mass donor star. We obtain a new radial velocity amplitude for the mass donor star of K2 = 202 +/- 3 km/s, which compares with the value of K2 = 135 +/- 3 km/s obtained in Stover, Robinson & Nather's classic 1981 study of EM Cyg. The revised value of the amplitude combined with a measurement of rotational broadening of the mass donor vsini = 140 +/- 6 km/s, leads to a new mass ratio of q = M2/M1 = 0.88 +/- 0.05. This solves a long standing problem with EM Cyg because Stover et al.'s measurements indicated a mass ratio q > 1, a value which should have led to dynamically unstable mass transfer for the secondary mass deduced by Stover et al. The revised value of the mass ratio combined with the orbital inclination i = 67 +/- 2 degrees leads to masses of 0.99 +/- 0.12 Msun and 1.12 +/- 0.08 Msun for the mass donor and white dwarf respectively. The mass donor is evolved, since it has a later spectral type (K3) than its mass would imply. We discuss whether the K star could be physically associated with EM Cyg or not, and present the results of the spectroscopic study.Comment: 10 pages, 12 figures, accepted for publication in MNRA

Young stellar object jet models: From theory to synthetic observations

Astronomical observations, analytical solutions and numerical simulations have provided the building blocks to formulate the current theory of young stellar object jets. Although each approach has made great progress independently, it is only during the last decade that significant efforts are being made to bring the separate pieces together. Building on previous work that combined analytical solutions and numerical simulations, we apply a sophisticated cooling function to incorporate optically thin energy losses in the dynamics. On the one hand, this allows a self-consistent treatment of the jet evolution and on the other, it provides the necessary data to generate synthetic emission maps. Firstly, analytical disk and stellar outflow solutions are properly combined to initialize numerical two-component jet models inside the computational box. Secondly, magneto-hydrodynamical simulations are performed in 2.5D, following properly the ionization and recombination of a maximum of $29$ ions. Finally, the outputs are post-processed to produce artificial observational data. The first two-component jet simulations, based on analytical models, that include ionization and optically thin radiation losses demonstrate promising results for modeling specific young stellar object outflows. The generation of synthetic emission maps provides the link to observations, as well as the necessary feedback for the further improvement of the available models.Comment: accepted for publication A&A, 20 pages, 11 figure

Formation of Episodic Magnetically Driven Radiatively Cooled Plasma Jets in the Laboratory

We report on experiments in which magnetically driven radiatively cooled plasma jets were produced by a 1 MA, 250 ns current pulse on the MAGPIE pulsed power facility. The jets were driven by the pressure of a toroidal magnetic field in a ''magnetic tower'' jet configuration. This scenario is characterized by the formation of a magnetically collimated plasma jet on the axis of a magnetic ''bubble'', confined by the ambient medium. The use of a radial metallic foil instead of the radial wire arrays employed in our previous work allows for the generation of episodic magnetic tower outflows which emerge periodically on timescales of ~30 ns. The subsequent magnetic bubbles propagate with velocities reaching ~300 km/s and interact with previous eruptions leading to the formation of shocks.Comment: 6 pages, 5 figures. Accepted for publication in Astrophysics & Space Scienc

Diagenesis in salt dome roof strata: barite - calcite assemblage in Jebel Madar, Oman

Halokinesis causes a dynamic structural evolution with the development of faults and fractures, which can act as either preferential fluid pathways or barriers. Reconstructing reactive fluid flow in salt dome settings remains a challenge. This contribution presents for the first time a spatial distribution map of diagenetic phases in a salt dome in northern Oman. Our study establishes a clear link between structural evolution and fluid flow leading to the formation of diagenetic products (barite and calcite) in the salt dome roof strata. Extensive formation of diagenetic products occurs along NNE-SSW to NE-SW faults and fractures, which initiated during the Santonian (Late Cretaceous) and were reactivated in the Miocene, but not along the E-W fault, which was generated during Early Paleocene time. We propose that the diagenetic products formed by mixing of a warm (100 °C) saline (17 wt% NaCl eq.) 87Sr enriched (87Sr/86Sr: 0.71023) fluid with colder (35 °C) meteoric fluid during Miocene to Pleistocene. The stable sulphur and strontium isotope composition and fluid inclusion data indicate that a saline fluid, with sulfate source derived from the Ara Group evaporite and Haima Supergroup layers, is the source for barite formation at about 100 °C, predominantly at fault conjunctions and minor faults away from the main graben structure in the dome. In the Miocene, the saline fluid probably ascended along a halokinesis-related fault due to fluid overpressure (due to the rising salt and impermeable layers in the overlying stratigraphic sequence), and triggered the formation of barite due mixing with barium-rich fluids, accompanied by a drop in temperature. Subsequently, evolving salt doming with associated fault activity and erosion of the Jebel allows progressively more input of colder meteoric fluids, which mix with the saline warmer fluid, as derived from stable isotope data measured in the progressively younger barite-associated calcite, fault zone calcite and macro-columnar calcite. The reconstructed mixing model indicates a 50/50 to 90/10 meteoric/saline fluid mixing ratio for the formation of fault zone calcite, and a 10 times higher concentration of carbon in the saline fluid end member compared to the meteoric fluid end member. The presented mixing model of salt-derived fluids with meteoric fluids is suggested to be a general model applicable to structural diagenetic evolution of salt domes world wide

\u3cem\u3eTrichodysplasia Spinulosa\u3c/em\u3e-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids

Trichodysplasia spinulosa-associated Polyomavirus (TSPyV) was isolated from a patient suffering from trichodysplasia spinulosa, a skin disease that can appear in severely immunocompromised patients. While TSPyV is one of the five members of the polyomavirus family that are directly linked to a human disease, details about molecular recognition events, the viral entry pathway, and intracellular trafficking events during TSPyV infection remain unknown. Here we have used a structure-function approach to shed light on the first steps of TSPyV infection. We established by cell binding and pseudovirus infection studies that TSPyV interacts with sialic acids during attachment and/or entry. Subsequently, we solved high-resolution X-ray structures of the major capsid protein VP1 of TSPyV in complex with three different glycans, the branched GM1 glycan, and the linear trisaccharides α2,3- and α2,6-sialyllactose. The terminal sialic acid of all three glycans is engaged in a unique binding site on TSPyV VP1, which is positioned about 18 Å from established sialic acid binding sites of other polyomaviruses. Structure-based mutagenesis of sialic acid-binding residues leads to reduction in cell attachment and pseudovirus infection, demonstrating the physiological relevance of the TSPyV VP1-glycan interaction. Furthermore, treatments of cells with inhibitors of N-, O-linked glycosylation, and glycosphingolipid synthesis suggest that glycolipids play an important role during TSPyV infection. Our findings elucidate the first molecular recognition events of cellular infection with TSPyV and demonstrate that receptor recognition by polyomaviruses is highly variable not only in interactions with sialic acid itself, but also in the location of the binding site

Immunohistochemical localization of hTERT protein in human tissues

Telomerase is a ribonucleoprotein complex mainly composed of a reverse transcriptase catalytic subunit (telomerase reverse transcriptase gene, hTERT) that copies a template region of its RNA subunit to the end of the telomere. For detecting telomerase activity in a tissue specimen the TRAP assay is a relatively sensitive and specific method, but it can be used only on fresh tissue extracts and offers no information at the single cell level. Immunohistochemistry (IHC) allows to detect hTERT protein expression at an individual cell level in human tissues. We have tested commercially available anti-hTERT antibodies in formalin-fixed and paraffin-embedded human tissues by IHC. Only one monoclonal antibody (NCL-hTERT; Novacastra) was sufficiently specific and this was applied to human tissues in which telomerase activity had been shown by TRAP assay and hTERT mRNA expression by RT-PCR. hTERT protein localized diffusely in the nucleoplasm and more intensely in the nucleoli of cancer cells and proliferating normal cells. Mitotic cells showed diffuse staining of the entire cell. Granular cytoplasmic staining was occasionally found in some tumor cells. In telomerase-positive tumors not all the tumor cells showed hTERT immunoreactivity. A significantly heterogeneous hTERT protein expression was observed in human tumor tissues. The hTERT immunostaining in fixed tissues was concordant with telomerase activity and hTERT mRNA expression in corresponding non-fixed samples. Quantitative RT-PCR of microdissected sections showed that hTERT mRNA expression was higher in cells with nuclear expression than in those with cytoplasmic expression. Double staining with the M30 antibody showed that a subpopulation of hTERT-negative cells is apoptotic. We conclude that: (1) hTERT protein can be detected by IHC in fixed human tissues, but the choice of the antibody, tissue processing, and reaction conditions are critical, (2) hTERT protein localizes in the nucleoplasm, more strongly in the nucleolus, and occasionally in the cytoplasm, (3) telomerase-positive tumors show significant heterogeneity of hTERT protein expression, and (4) a subpopulation of hTERT protein negative tumor cells is identified as apoptotic cell

The spectrum of resistance in SR/CR mice: the critical role of chemoattraction in the cancer/leukocyte interaction

<p>Abstract</p> <p>Background</p> <p>Spontaneous regression/complete resistance (SR/CR) mice are a unique colony of mice that possess an inheritable, natural cancer resistance mediated primarily by innate cellular immunity. This resistance is effective against sarcoma 180 (S180) at exceptionally high doses and these mice remain healthy.</p> <p>Methods</p> <p>In this study, we challenged SR/CR mice with additional lethal transplantable mouse cancer cell lines to determine their resistance spectrum. The ability of these transplantable cancer cell lines to induce leukocyte infiltration was quantified and the percentage of different populations of responding immune cells was determined using flow cytometry.</p> <p>Results</p> <p>In comparison to wild type (WT) mice, SR/CR mice showed significantly higher resistance to all cancer cell lines tested. However, SR/CR mice were more sensitive to MethA sarcoma (MethA), B16 melanoma (B16), LL/2 lung carcinoma (LL/2) and J774 lymphoma (J774) than to sarcoma 180 (S180) and EL-4 lymphoma (EL-4). Further mechanistic studies revealed that this lower resistance to MethA and LL/2 was due to the inability of these cancer cells to attract SR/CR leukocytes, leading to tumor cell escape from resistance mechanism. This escape mechanism was overcome by co-injection with S180, which could attract SR/CR leukocytes allowing the mice to resist higher doses of MethA and LL/2. S180-induced cell-free ascites fluid (CFAF) co-injection recapitulated the results obtained with live S180 cells, suggesting that this chemoattraction by cancer cells is mediated by diffusible molecules. We also tested for the first time whether SR/CR mice were able to resist additional cancer cell lines prior to S180 exposure. We found that SR/CR mice had an innate resistance against EL-4 and J774.</p> <p>Conclusions</p> <p>Our results suggest that the cancer resistance in SR/CR mice is based on at least two separate processes: leukocyte migration/infiltration to the site of cancer cells and recognition of common surface properties on cancer cells. The infiltration of SR/CR leukocytes was based on both the innate ability of leukocytes to respond to chemotactic signals produced by cancer cells and on whether cancer cells produced these chemotactic signals. We found that some cancer cells could escape from SR/CR resistance because they did not induce infiltration of SR/CR leukocytes. However, if infiltration of leukocytes was induced by co-injection with chemotactic factors, these same cancer cells could be effectively recognized and killed by SR/CR leukocytes.</p