Slow-light enhanced optical detection in liquid-infiltrated photonic crystals

Slow-light enhanced optical detection in liquid-infiltrated photonic crystals is theoretically studied. Using a scattering-matrix approach and the Wigner-Smith delay time concept, we show that optical absorbance benefits both from slow-light phenomena as well as a high filling factor of the energy residing in the liquid. Utilizing strongly dispersive photonic crystal structures, we numerically demonstrate how liquid-infiltrated photonic crystals facilitate enhanced light-matter interactions, by potentially up to an order of magnitude. The proposed concept provides strong opportunities for improving existing miniaturized absorbance cells for optical detection in lab-on-a-chip systems.Comment: Paper accepted for the "Special Issue OWTNM 2007" edited by A. Lavrinenko and P. J. Robert

Drosophila BubR1 Is Essential for Meiotic Sister-Chromatid Cohesion and Maintenance of Synaptonemal Complex

SummaryThe partially conserved Mad3/BubR1 protein is required during mitosis for the spindle assembly checkpoint (SAC). In meiosis, depletion causes an accelerated transit through prophase I and missegregation of achiasmate chromosomes in yeast [1], whereas in mice, reduced dosage leads to severe chromosome missegregation [2]. These observations indicate a meiotic requirement for BubR1, but its mechanism of action remains unknown. We identified a viable bubR1 allele in Drosophila resulting from a point mutation in the kinase domain that retains mitotic SAC activity. In males, we demonstrate a dose-sensitive requirement for BubR1 in maintaining sister-chromatid cohesion at anaphase I, whereas the mutant BubR1 protein localizes correctly. In bubR1 mutant females, we find that both achiasmate and chiasmate chromosomes nondisjoin mostly equationally consistent with a defect in sister-chromatid cohesion at late anaphase I or meiosis II. Moreover, mutations in bubR1 cause a consistent increase in pericentric heterochromatin exchange frequency, and although the synaptonemal complex is set up properly during transit through the germarium, it is disassembled prematurely in prophase by stage 1. Our results demonstrate that BubR1 is essential to maintain sister-chromatid cohesion during meiotic progression in both sexes and for normal maintenance of SC in females

Blood group substances as differentiation markers in human dento-gingival epithelium

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65594/1/j.1600-0765.1987.tb02054.x.pd

A 200-year 210Pb record from Greenland

A continuous profile of 210Pb activity extending back to 1766 has been developed for a firn/ice core collected at Site D in central Greenland in 1984. Unexpectedly high activities of 210Pb were found at the base of this core (0.032 pCi kg−1 in samples more than 200 years old), calling into question the common assumption that supported 210Pb can be neglected when constructing chronologies in glacial snow and ice. It is problematic to assert that all of the 210Pb measured at depth should be attributed to the supported fraction, given previous estimates of dust loading in Greenland ice cores. However, even if an estimated constant value of 0.032 pCi supported210Pb kg−1 is subtracted from the measured values to estimate excess 210Pb, the 210Pb chronology for Site D yields ages that are significantly younger (mean accumulation rate too high) than an independent depth-age scale based on annual layer counting. It is apparent that the flux of excess and/or supported 210Pb to this site must have decreased over the past 2 centuries, with decreasing trends in both fractions most likely. Previously published 210Pb profiles for cores from Summit and Dye 3, Greenland, show similar trends, which had been interpreted as decreasing fluxes of excess 210Pb only. For all three sites, it is not possible to separate variations in the fluxes of the excess and supported fractions of 210Pb, but variations in the total 210Pb flux will impact 210Pb-based chronologies generally if these variations have not been restricted to the Greenland ice sheet

Magnetization of Greenland ice and its relationship with dust content

[1] We estimate the concentration of fine magnetic particles in ice samples from the North Greenland Ice Core Project core from the central Greenland ice sheet, using lowtemperature (77K) isothermal remanent magnetization (IRM) analysis and compare it with the mass concentration of aerosol dust. Samples were taken from six climatic intervals, spanning the time from the Holocene (Preboreal) back to the Last Glacial Dansgaard/ Oeschger cycle 5. The mean IRM intensity of the ice varies by a factor of 3 from glacial to interglacial stages, being lower during interglacials. The IRM acquisition curves of the ice do not quite saturate at the maximum available field of 0.8 T and show a relatively broad coercivity, which is compatible with a mixture of maghemite or magnetite and hematite. Comparison of the IRM intensity and total dust mass shows a remarkably good correlation but also reveals a large background magnetization, which may be essentially constant over the different climatic stages. IRM suggests that the dust properties are independent of the background signal and that the dust aerosol has a magnetization within about 30% of pristine loess from the Chinese Loess Plateau, which is considered to have the same source in the same east Asian deserts as dust in Greenland ice. Ice contamination and the flux of extraterrestrial dust particles were considered in order to explain the origin of the background magnetization. Nevertheless, we could not find a convincing explanation for this signal, which represents a considerable part of the IRM signal and is the dominant component during interglacial intervals, without invoking the presence of undetected dust mass. The alternative hypothesis of a varying magnetization of the ice dust at different climatic periods would suggest that different sources of aerosol are active during different climatic periods. This, however, has not proven to be the case so far for studies of the provenance of dust in Greenland ice

PB1 as a potential target for increasing the breadth of T-cell mediated immunity to Influenza A

Recently, we showed that combined intranasal and subcutaneous immunization with a non-replicating adenoviral vector expressing NP of influenza A, strain PR8, induced long-standing protection against a range of influenza A viruses. However, H-2(b) mice challenged with an influenza A strain mutated in the dominant NP(366) epitope were not efficiently protected. To address this problem, we envision the use of a cocktail of adenovectors targeting different internal proteins of influenza A virus. Consequently, we investigated the possibility of using PB1 as a target for an adenovector-based vaccine against influenza A. Our results showed that PB1 is not as immunogenic as the NP protein. However, by tethering PB1 to the murine invariant chain we were able to circumvent this problem and raise quite high numbers of PB1-specific CD8(+) T cells in the circulation. Nevertheless, mice immunized against PB1 were not as efficiently protected against influenza A challenge as similarly NP-vaccinated animals. The reason for this is not a difference in the quality of the primed cells, nor in functional avidity. However, under similar conditions of immunization fewer PB1-specific cells were recruited to the airways, and surface expression of the dominant PB1 peptide, PB1(703), was less stable than in the case of NP(366)

Holocene volcanic history as recorded in the sulfate stratigraphy of the European Project for Ice Coring in Antarctica Dome C (EDC96) ice core

A detailed history of Holocene volcanism was reconstructed using the sulfate record of the European Project for Ice Coring in Antarctica Dome C (EDC96) ice core. This first complete Holocene volcanic record from an Antarctic core provides a reliable database to compare with long records from Antarctic and Greenland ice cores. A threshold method based on statistical treatment of the lognormal sulfate flux distribution was used to differentiate volcanic sulfate spikes from sulfate background concentrations. Ninety-six eruptions were identified in the EDC96 ice core during the Holocene, with a mean of 7.9 events per millennium. The frequency distribution (events per millennium) showed that the last 2000 years were a period of enhanced volcanic activity. EDC96 volcanic signatures for the last millennium are in good agreement with those recorded in other Antarctic ice cores. For older periods, comparison is in some cases less reliable, mainly because of dating uncertainties. Sulfate depositional fluxes of individual volcanic events vary greatly among the different cores. A volcanic flux normalization (volcanic flux/Tambora flux ratio) was used to evaluate the relative intensity of the same event recorded at different sites in the last millennium. Normalized flux variability for the same event showed the highest value in the 1100–1500 AD period. This pattern could mirror changes in regional transport linked to climatic variations such as slight warming stages in the Southern Hemisphere (Southern Hemisphere Medieval Warming–like period?)