Gα16, a G Protein α Subunit Specifically Expressed in Hematopoietic Cells

Signal-transduction pathways mediated by guanine nucleotide-binding regulatory proteins (G proteins) determine many of the responses of hematopoietic cells. A recently identified gene encoding a G protein α subunit, Gα16, is specifically expressed in human cells of the hematopoietic lineage. The Gα16 cDNA encodes a protein with predicted Mr of 43,500, which resembles the Gq class of α subunits and does not include a pertussis toxin ADP-ribosylation site. In comparison with other G protein α subunits, the Gα16 predicted protein has distinctive amino acid sequences in the amino terminus, the region A guanine nucleotide-binding domain, and in the carboxyl-terminal third of the protein. Cell lines of myelomonocytic and T-cell phenotype express the Gα16 gene, but no expression is detectable in two B-cell lines or in nonhematopoietic cell lines. Gα16 gene expression is down-regulated in HL-60 cells induced to differentiate to neutrophils with dimethyl sulfoxide. Antisera generated from synthetic peptides that correspond to two regions of Gα16 specifically react with a protein of 42- to 43-kDa in bacterial strains that overexpress Gα16 and in HL-60 membranes. This protein is decreased in membranes from dimethyl sulfoxide-differentiated HL-60 cells and is not detectable in COS cell membranes. The restricted expression of this gene suggests that Gα16 regulates cell-type-specific signal-transduction pathways, which are not inhibited by pertussis toxin

Multiwavelength observations of the Be/X-ray binary 4U1145-619

We report optical and infrared observations of the massive X-ray binary system 4U1145-619 (V801 Cen) which show that the circumstellar disc of the Be star component is in decline. Infrared J,H,K,L magnitudes of V801Cen have been monitored from 1993 March to 1996 April. H alpha spectra have been obtained throughout the same period. We find that both the infrared excess and the Balmer emission have been in decline throughout the period of observations. A 13 year optical and X-ray history of the source has been collated, revealing a possible correlation between the optical and X-ray activity. In addition, we have used u,v,b,y,beta indices, corrected for both circumstellar and interstellar effects, to calculate the physical parameters of the underlying B star.Comment: 8 pages postscript. Accepted by MNRA

Integrating Algaculture into Small Wastewater Treatment Plants: Process Flow Options and Life Cycle Impacts

Algaculture has the potential to be a sustainable option for nutrient removal at wastewater treatment plants. The purpose of this study was to compare the environmental impacts of three likely algaculture integration strategies to a conventional nutrient removal strategy. Process modeling was used to determine life cycle inventory data and a comparative life cycle assessment was used to determine environmental impacts. Treatment scenarios included a base case treatment plant without nutrient removal, a plant with conventional nutrient removal, and three other cases with algal unit processes placed at the head of the plant, in a side stream, and at the end of the plant, respectively. Impact categories included eutrophication, global warming, ecotoxicity, and primary energy demand. Integrating algaculture prior to activated sludge proved to be most beneficial of the scenarios considered for all impact categories; however, this scenario would also require primary sedimentation and impacts of that unit process should be considered for implementation of such a system

Results from the Blazar Monitoring Campaign at the Whipple 10m Gamma-ray Telescope

In September 2005, the observing program of the Whipple 10 m gamma-ray telescope was redefined to be dedicated almost exclusively to AGN monitoring. Since then the five Northern Hemisphere blazars that had already been detected at Whipple are monitored routinely each night that they are visible. Thanks to the efforts of a large number of multiwavelength collaborators, the first year of this program has been very successful. We report here on the analysis of Markarian 421 observations taken from November, 2005 to May, 2006 in the gamma-ray, X-ray, optical and radio bands.Comment: 4 pages; contribution to the 30th International Cosmic Ray Conference, Merida, Mexico, July 200