Impacts of lower speed limits in South Australia

ustralia has recently undergone a change of urban speed limits in most of its jurisdictions. The political and social shift in attitudes required for this change is significant in a country that has a strong reliance on the use of the private motor vehicle. At present five states and one territory have lowered the speed limit in urban areas from 60km/h to 50km/h. Of the remaining two states and territories, one has already implemented lower speed limits (40km/h) in small areas within cities. In the majority of cases, the only criterion for judging the success of such schemes is an observed reduction in both speeds and crash numbers. This paper reports on a more holistic assessment of such schemes taking into account factors in addition to speed and crashes including: traffic volume displacement, physical road network characteristics, environmental factors, community ownership and acceptance, enforcement effort and impact on travel times. The research work has included the analysis of extensive traffic data, community surveys and focus groups, the collection of environmental and travel time data from an instrumented probe vehicle and the computer modelling of road networks. The work reported is based on over 10 years of working with a 40km/h lower urban speed limit area in South Australia. The paper expands the notion of using speed and crash outcomes as the only criteria for measuring the success of lower speed limit schemes. Document type: Articl

Transcriptome dynamics of a broad host-range cyanophage and its hosts

Cyanobacteria are highly abundant in the oceans and are constantly exposed to lytic viruses. The T4-like cyanomyoviruses are abundant in the marine environment and have broad host-ranges relative to other cyanophages. It is currently unknown whether broad host-range phages specifically tailor their infection program for each host, or employ the same program irrespective of the host infected. Also unknown is how different hosts respond to infection by the same phage. Here we used microarray and RNA-seq analyses to investigate the interaction between the Syn9 T4-like cyanophage and three phylogenetically, ecologically and genomically distinct marine Synechococcus strains: WH7803, WH8102 and WH8109. Strikingly, Syn9 led a nearly identical infection and transcriptional program in all three hosts. Different to previous assumptions for T4-like cyanophages, three temporally regulated gene expression classes were observed. Furthermore, a novel regulatory element controlled early-gene transcription, and host-like promoters drove middle gene transcription, different to the regulatory paradigm for T4. Similar results were found for the P-TIM40 phage during infection of Prochlorococcus NATL2A. Moreover, genomic and metagenomic analyses indicate that these regulatory elements are abundant and conserved among T4-like cyanophages. In contrast to the near-identical transcriptional program employed by Syn9, host responses to infection involved host-specific genes primarily located in hypervariable genomic islands, substantiating islands as a major axis of phage-cyanobacteria interactions. Our findings suggest that the ability of broad host-range phages to infect multiple hosts is more likely dependent on the effectiveness of host defense strategies than on differential tailoring of the infection process by the phage

An effective tool for advanced traveller's information systems development

Kun Zhang, Jeremy Woolley, Branco Stazi

The excludon: a new concept in bacterial antisense RNA-mediated gene regulation

International audienceIn recent years, non-coding RNAs have emerged as key regulators of gene expression. Among these RNAs, the antisense RNAs (asRNAs) are particularly abundant, but in most cases the function and mechanism of action for a particular asRNA remains elusive. Here, we highlight a recently discovered paradigm termed the excludon, which defines a genomic locus encoding an unusually long asRNA that spans divergent genes or operons with related or opposing functions. Because these asRNAs can inhibit the expression of one operon while functioning as an mRNA for the adjacent operon, they act as fine-tuning regulatory switches in bacteria