Tricarbonyl technetium(I) complexes with different phosphate ligands

The possibilities for labelling of different phosphate ligands with technetium-99m through [99m Tc(CO)3(H2O)3] + precursor were investigated. The HPLC results have shown that hydrophilic organometallic [ 99mTc(CO)3(H2O)3] + precursor allows forming of Tc (I) complexes with different phosphate ligands, based on the tricarbonyltechnetium (I) core.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

99mTc-hexakis-(2-metoxy-isobutyl-isonitrile) ( 99mTc-MIBI) a new myocardial imaging agent: synthesis of MIBI, optimising conditions for radiolabelling with 99mTc at high radiochemical purity and in vivo behavior

99mTc-MIBI is a promising radiopharmaceutical for myocardial perfusion imaging agent, but it has also shown good results in identifying several types of tumors, such as breast, lung and thyroid cancers. It is a lipophilic, cationic technetium (1) complex. In this paper a complete study on the synthesis of 2-metohy-isobutyl-isonitrile (MIBI) as well as a formulation of a lyophilized kit for labeling with 99mTc is presented. Investigation on effective factors as well as finding out the optimum parameters to obtain the highest labelling efficiency and radiochemical purity of 99mTc-MIBI complex were performed. The radiochemical purity of the labelled preparation was high (>95%). Biodistribution study performed in health male Wistar rats showed satisfactory biokinetics results. 99mTc-MIBI was accumulated in sufficient amount into the hearth tissue for myocardial perfusion imaging. MIBI in kit formulation was found to be stable and also safe for administration.Physical chemistry 2006 : 8th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-29 September 200

Ispitivanje uticaja nanofotoničnih tvrdih gaspropusnih kontaktnih sočiva na fiziološki rastvor akvafotomikom i opto-magnetnom imidžing spektroskopijom

Contact lenses represent biomaterials whose main purpose is to correct the specific refractive anomaly of the eye. Since the visible light on its way to the perceptive part of the eye has to pass through the contact lens, the characteristics of the materials can significantly modify it. Biocompatibility of the lens surface is one of the most important issues in achieving contact lens wear without problems. We have developed new nanophotonic contact lens materials by adding nanoparticles of fullerene and their derivatives into standard PMMA RGP material. The aim of our investigation was to compare the influences of these materials on saline which is similar to tear film. We used NIR spectroscopy based on 12 vibration modes, called Aquaphotomics and Opto-magnetic imaging (OMI) spectroscopy as methods for characterizing the samples. The acquired spectrums were commented and compared with the standard contact lens material, which was analyzed by the same method.Kontaktna sočiva predstavljaju biomaterijale čija je glavna svrha ispravljanje specifičnih refraktivnih anomalija oka. S obzirom da vidljiva svetlost na svom putu do perceptivnog dela oka mora da prođe kroz kontaktno sočivo, karakteristike materijala mogu značajno da je izmene. Biokompatibilnost površine sočiva je jedno od najvažnijih pitanja u postizanju nošenja kontaktnih sočiva bez problema. Razvijeni su novi nanofotonični materijali za kontaktna sočiva dodavanjem nanočestica fulerena i njihovih derivata u standardni PMMA materijal za tvrda gas propusna kontaktna sočiva. Cilj našeg istraživanja je bilo poređenje uticaja ovih materijala na fiziološki rastvor koji je sličan suznom filmu. Za karakterizaciju uzoraka koristili smo blisku infracrvenu spektroskopiju zasnovanu na 12 vibracionih modova, poznatiju kao Akvafotomika. Dobijeni spektri su komentarisani i upoređeni sa standardnim materijalom za kontaktna sočiva, koji je analiziran istom metodom

ABO histo-blood groups and Rh systems in relation to malignant tumors of the digestive tract in Bosnia and Herzegovina

The distribution of ABO blood groups and the Rhesus factor was analyzed in 279 patients who suffered from malignant tumors of the digestive system. Patients were registered retrospectively in the Gastroenterohepatology Clinic, Clinical Center, University of Sarajevo over a discontinuous period of 88 months. From the results obtained, it was concluded that: (a) men became ill from gastric cancer significantly more frequently than women; (b) the frequency of liver carcinoma was three times higher than the global frequency and the frequency neighboring ethnic groups; and (c) patients with blood group B and patients with RhD(-) exhibited a significantly higher proportion of disease.Analizirana je distribucija krvnih grupa ABO sistema i Rezus faktora kod 279 pacijenata obolelih od malignih tumora digestivnog sistema. Pacijenti su registrovani retrospektivno u Gastroenterohepatološkoj klinici Kliničkog centra Univerziteta u Sarajevu u diskontinuitetu tokom 88 meseci (1987-1998). Na osnovu analizirane populacije pacijenata zaključeno je da: (a) muškarci značajno češće oboljevaju od kancera želuca u odnosu na žene; (b) učestalost karcinoma jetre je tri puta veća upoređenju sa učestalošću ovog oboljenja u svetu i susednim zemljama; (c) pacijenti sa V krvnom grupom i pacijenti sa RhD(-) su u značajnom stepenu češći u ispitivanoj populaciji obolelih od očekivanog.Projekat ministarstva br. 143010 i 14301

New Values of Teucrium species: in Vitro Study of Cytotoxic Activities of Secondary Metabolites

The cytotoxicity of seven Teucrium species, a long time ago used as a food spices, for beverages and teas preparing, as well as therapeutics for digestive and respiratory diseases, were examined against human cervix adenocarcinoma HeLa, human melanoma Fem-x, human chronic myelogenous leukemia K562 and human breast adenocarcinoma MDA-MB-361 cells. MTT assay was used for determination of target cell survival. The most prominent cytotoxic effect was observed against K562 cells, especially by T. scordioides, T. montanum and T. botrys. All Teucrium extracts showed good cytotoxic activity on HeLa cells, but very low cytotoxic effect on MDA-MB-361 cells. In addition, the cytotoxic activities of T. scordioides and T. montanum extract were tested on healthy resting and phytohaemagglutinin-stimulated peripheral blood mononuclear cells (PHA-stimulated PBMC). T. scordioides and T. montanum extracts at concentration of 200 µg/ml reduced the resting PBMC and PHA-stimulated PBMC survival up to 10% and 20%, while the reduction of K562 cell survival at the same concentration of extracts was 94% and 97%, respectively. These results point to selectivity in their antitumor actions. Teucrium species can be regarded as promising candidates for natural plant sources of effective biological compounds as a supplements in the food industry, as well as for therapeutic use

Study of iodine (n) and tin (p) doped Sb2S3 nanoparticles by detail X-ray photoelectron spectroscopy

X-ray photoelectron spectroscopy (XPS) measurements were used for analyzing the incorporation of iodine (I) and tin (Sn) into the stibnite (Sb2 S3 ) lattice obtained via the hot- injection method. The X-ray diffraction (XRD) technique revealed the visible presence of one phase, the pure orthorhombic structure of Sb 2 S3 with the Pnma group. Scanning electron microscopy (SEM) showed long columnar structures with length of few nanometers and diameter of about 150 nm.The incorporation of I and Sn into Sb 2 S3 was verified by comparing the XPS spectra of the non-doped Sb2 S3 and iodine and tin-doped samples, by the distinctive appearance of characteristic 3d peaks of iodine and tin. As well, the relative amounts of I and Sn dopants were determined from the I 3d5/2 and Sn 3d 5/2 , respectively. The obtained, lesser than expected, amount of dopants is likely due to a possible weakening of I and Sn signals. Shifting of the valence band towards higher (I-doped Sb2S3 ) or lower (Sn-doped Sb2 S3 ) energies, related to the non-doped sample, also confirmed the successful incorporation of dopant atoms in the Sb2 S3 lattice.IX Serbian Ceramic Society Conference - Advanced Ceramics and Application : new frontiers in multifunctional material science and processing : program and the book of abstracts; September 20-21, 2021; Belgrad

ارزیابی کیفیت آزمون های چهار گزینه ای دروس تئوری عمومي گروه اندودانتیکس دانشکده دندانپزشکی قزوین در طی سال های تحصیلی 1388 تا 1396

Svrha je ovoga rada bila ispitati citotoksični, proapoptotski, antimigratorni i pro-antioksidacijski učinak metanolnih, acetonskih i etil-acetatnih ekstrakata dvaju vrsta lišaja, i to: Pseudevernia furfuracea i Platismatia glauca na tumorske stanične linije debelog crijeva (HCT-116 i SW-480). Citotoksični efekti na tumorskim staničnim linijama debelog crijeva uspoređeni su s onima na staničnoj liniji normalnih humanih fibroblasta (MRC-5). Citotoksičnost je ispitana MTT testom, stanična migracija Transwell esejom, dok je apoptoza praćena fluorescentnom metodom pomoću akridin narančastog bojila i etidijevog bromida. Prooksidacijski/antioksidacijski učinak praćen je spektrofotometrijskim mjerenjem koncentracije redoks parametara. Ispitani ekstrakti imali su značajan citotoksični učinak na tumorske stanične linije, bez zapaženog utjecaja na normalnu staničnu liniju. Najjača citotoksičnost postignuta je nakon 72 h obrade staničnih linija ekstraktima vrste P. furfuracea (IC50= (21,2±1.3) µg/mL pri obradi HCT-116 stanica etil-acetatnim ekstraktom i IC50=(51.3±0.8) µg/mL pri obradi stanica SW-480 acetonskim ekstraktom). Ekstrakti P. furfuracea imali su značajan proapoptotski i prooksidacijski učinak, dok je metanolni ekstrakt te vrste imao najjači antimigratorni učinak na ispitane tumorske stanične linije. Svi ispitani ekstrakti vrste P. glauca imali su znatan citotoksični učinak na stanice HCT-116 nakon 72 h tretmana (IC50<40 μg/mL), dok su metanolni i acetonski ekstrakti imali citotoksični učinak na stanice SW-480 nakon 24 h, s izraženom proapoptotskom/nekrotskom aktivnošću. Svi navedeni efekti posljedica su induciranog oksidacijskog stresa u tretiranim stanicama. Može se zaključiti da ekstrakti lišaja bitno mijenjaju vijabilnost i migratorni potencijal ispitanih tumorskih staničnih linija karcinoma debelog crijeva. Dobiveni rezultati pokazuju da su stanice HCT-116 osetljivije na ispitane ekstrakte, pri čemu ekstrakti vrste P. furfuracea pokazuju bolji proapototski i antimigratorni učinak. Ispitane vrste lišaja mogu se smatrati izvorima antikancerogenih aktivnih tvari.The aim of this study is to investigate cytotoxic, proapoptotic, antimigratory and pro-antioxidant effects of methanol, acetone and ethyl acetate extracts of lichens Pseudevernia furfuracea and Platismatia glauca on colorectal cancer (HCT-116 and SW-480) cell lines. We compared the cytotoxic effects on colorectal cancer cells with the effects obtained from normal human fibroblast (MRC-5) cell line. Tetrazolium (MTT) test evaluated the cytotoxic effects, Transwell assay evaluated cell migration, acridine orange/ethidium bromide (AO/EB) fluorescent method followed the apoptosis, while prooxidant/antioxidant effects were determined spectrophotometrically through concentration of redox parameters. The tested extracts showed considerable cytotoxic effect on cancer cells with no observable cytotoxic effect on normal cells. Ethyl acetate and acetone extract of P. furfuracea induced the highest cytotoxicity (IC50=(21.2±1.3) μg/mL on HCT-116, and IC50=(51.3±0.8) μg/mL on SW-480 cells, respectively, after 72 h), with noteworthy apoptotic and prooxidant effects, and antimigratory potential of methanol extract. P. glauca extracts induced cytotoxic effects on HCT-116 cells after 72 h (IC50<40 μg/mL), while only methanol and acetone extracts had cytotoxic effects on SW-480 cells after 24 h, with proapoptotic/necrotic activity, as a consequence of induced oxidative stress. In conclusion, lichen extracts changed to a great extent cell viability and migratory potential of colorectal cancer cell lines. HCT-116 cells were more sensitive to treatments, P. furfuracea had better proapoptotic and antimigratory effects, and both investigated lichen species might be a source of substances with anticancer activity

TWO-DIMENSIONAL FLUID MODELING OF DC GLOW DISCHARGE IN ARGON AT LOW PRESSURE

The modeling of DC glow discharge in argon at the pressure and inter-electrode distance was performed for different voltages and glow currents. For the first time, argon glow discharge is modeled using a two dimensional (2D) fluid model with non-local ionization. A detailed numerical procedure for 2D fluid modeling is given. The 2D profiles of particles number densities and electric potential obtained from the fluid model with non-local ionization and the simple fluid model are presented and compared. DVODIMENZIONI FLUIDNI MODEL JEDNOSMERNOG TINJAVOG PRAŽNJENJA U ARGONU NA NISKOM PRITISKUU radu je izvršeno modelovanje jednosmernog tinjavog pražnjenja u argonu na pritisku i medjuelektrodnom rastojanjupri različitim vrednostima primenjenih napona i radnih struja tinjavog pražnjenja. Po prvi put je modelovano tinjavo pražnjenje u argonu dvodimenzionim fluidnim modelom sa nelokalnom jonizacijom. U radu je data detaljna procedura za 2D fluidni model. Osim toga, izračunate su i  uporedjene 2D raspodele koncentracija čestica i električnog potencijala dobijenih pomoću fluidnog modela sa nelokalnom jonizacijom i  jednostavnog  fluidnog modela. HIGHLIGHTSThe modeling of DC glow discharge in argon was performed for different voltages and glow currents;Glow discharge was modeled using the two-dimensional (2D) simple fluid model and the fluid model with non-local ionization;A detailed numerical procedure for 2D fluid modeling is given;2D profiles of the particle number densities and the electric potential obtained from the fluid model with non-local ionization and the simple fluid model are presented and compared

TWO-DIMENSIONAL FLUID MODELING OF DC GLOW DISCHARGE IN ARGON AT LOW PRESSURE

The modeling of DC glow discharge in argon at the pressure and inter-electrode distance was performed for different voltages and glow currents. For the first time, argon glow discharge is modeled using a two dimensional (2D) fluid model with non-local ionization. A detailed numerical procedure for 2D fluid modeling is given. The 2D profiles of particles number densities and electric potential obtained from the fluid model with non-local ionization and the simple fluid model are presented and compared. DVODIMENZIONI FLUIDNI MODEL JEDNOSMERNOG TINJAVOG PRAŽNJENJA U ARGONU NA NISKOM PRITISKUU radu je izvršeno modelovanje jednosmernog tinjavog pražnjenja u argonu na pritisku i medjuelektrodnom rastojanjupri različitim vrednostima primenjenih napona i radnih struja tinjavog pražnjenja. Po prvi put je modelovano tinjavo pražnjenje u argonu dvodimenzionim fluidnim modelom sa nelokalnom jonizacijom. U radu je data detaljna procedura za 2D fluidni model. Osim toga, izračunate su i  uporedjene 2D raspodele koncentracija čestica i električnog potencijala dobijenih pomoću fluidnog modela sa nelokalnom jonizacijom i  jednostavnog  fluidnog modela. HIGHLIGHTSThe modeling of DC glow discharge in argon was performed for different voltages and glow currents;Glow discharge was modeled using the two-dimensional (2D) simple fluid model and the fluid model with non-local ionization;A detailed numerical procedure for 2D fluid modeling is given;2D profiles of the particle number densities and the electric potential obtained from the fluid model with non-local ionization and the simple fluid model are presented and compared