726 research outputs found
Guiding Trustful Behavior: The Role of Accessible Content and Accessibility Experiences
Trust has been identified as a key ingredient to the prosperity of close relationships, organizations, and societies. While research mainly focused on the antecedents and consequences of trust, much less is known about how individuals assess whether there are enough reasons to warrant trustful action. Two experiments explored the how and when of this assessment, suggesting that antecedents may not only be integrated as content information per se (as generally assumed), but in a feeling-based summary form. Specifically, our results show that the ease or difficulty associated with the identification of antecedents to trust may guide trustful behavior. Furthermore, it is shown that such a feeling-based influence is particularly likely to occur in conditions of personal certainty. Together these results extend prior research in the domains of trust and economic games, and further attest to the fundamental role cognitive feelings play in social life
Product assurance technology for custom LSI/VLSI electronics
The technology for obtaining custom integrated circuits from CMOS-bulk silicon foundries using a universal set of layout rules is presented. The technical efforts were guided by the requirement to develop a 3 micron CMOS test chip for the Combined Release and Radiation Effects Satellite (CRRES). This chip contains both analog and digital circuits. The development employed all the elements required to obtain custom circuits from silicon foundries, including circuit design, foundry interfacing, circuit test, and circuit qualification
Progress in the analysis of membrane protein structure and function
Structural information on membrane proteins is sparse, yet they represent an important class of proteins that is encoded by about 30% of all genes. Progress has primarily been achieved with bacterial proteins, but efforts to solve the structure of eukaryotic membrane proteins are also increasing. Most of the structures currently available have been obtained by exploiting the power of X-ray crystallography. Recent results, however, have demonstrated the accuracy of electron crystallography and the imaging power of the atomic force microscope. These instruments allow membrane proteins to be studied while embedded in the bi-layer, and thus in a functional state. The low signal-to-noise ratio of cryo-electron microscopy is overcome by crystallizing membrane proteins in a two- dimensional protein-lipid membrane, allowing its atomic structure to be determined. In contrast, the high signal-to- noise ratio of atomic force microscopy allows individual protein surfaces to be imaged at subnanometer resolution, and their conformational states to be sampled. This review summarizes the steps in membrane protein structure determination and illuminates recent progress. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies
Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range.
Investigation of potential hosts of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is crucial to understanding future risks of spillover and spillback. SARS-CoV-2 has been reported to be transmitted from humans to various animals after requiring relatively few mutations. There is significant interest in describing how the virus interacts with mice as they are well adapted to human environments, are used widely as infection models and can be infected. Structural and binding data of the mouse ACE2 receptor with the Spike protein of newly identified SARS-CoV-2 variants are needed to better understand the impact of immune system evading mutations present in variants of concern (VOC). Previous studies have developed mouse-adapted variants and identified residues critical for binding to heterologous ACE2 receptors. Here we report the cryo-EM structures of mouse ACE2 bound to trimeric Spike ectodomains of four different VOC: Beta, Omicron BA.1, Omicron BA.2.12.1 and Omicron BA.4/5. These variants represent the oldest to the newest variants known to bind the mouse ACE2 receptor. Our high-resolution structural data complemented with bio-layer interferometry (BLI) binding assays reveal a requirement for a combination of mutations in the Spike protein that enable binding to the mouse ACE2 receptor
Primary focal dystonia: evidence for distinct neuropsychiatric and personality profiles
Results on MeV-scale dark matter from a gram-scale cryogenic calorimeter operated above ground
Models for light dark matter particles with masses below 1 GeV/c are a
natural and well-motivated alternative to so-far unobserved weakly interacting
massive particles. Gram-scale cryogenic calorimeters provide the required
detector performance to detect these particles and extend the direct dark
matter search program of CRESST. A prototype 0.5 g sapphire detector developed
for the -cleus experiment has achieved an energy threshold of
eV, which is one order of magnitude lower than previous
results and independent of the type of particle interaction. The result
presented here is obtained in a setup above ground without significant
shielding against ambient and cosmogenic radiation. Although operated in a
high-background environment, the detector probes a new range of light-mass dark
matter particles previously not accessible by direct searches. We report the
first limit on the spin-independent dark matter particle-nucleon cross section
for masses between 140 MeV/c and 500 MeV/c.Comment: 6 pages, 6 figures, v3: ancillary files added, v4: high energy
spectrum (0.6-12keV) added to ancillary file
First results from the CRESST-III low-mass dark matter program
The CRESST experiment is a direct dark matter search which aims to measure
interactions of potential dark matter particles in an earth-bound detector.
With the current stage, CRESST-III, we focus on a low energy threshold for
increased sensitivity towards light dark matter particles. In this manuscript
we describe the analysis of one detector operated in the first run of
CRESST-III (05/2016-02/2018) achieving a nuclear recoil threshold of 30.1eV.
This result was obtained with a 23.6g CaWO crystal operated as a cryogenic
scintillating calorimeter in the CRESST setup at the Laboratori Nazionali del
Gran Sasso (LNGS). Both the primary phonon/heat signal and the simultaneously
emitted scintillation light, which is absorbed in a separate
silicon-on-sapphire light absorber, are measured with highly sensitive
transition edge sensors operated at ~15mK. The unique combination of these
sensors with the light element oxygen present in our target yields sensitivity
to dark matter particle masses as low as 160MeV/c.Comment: 9 pages, 9 figure
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