Trace metal accumulations in soils on and around ancient settlements in Greece.

Classical and Mediterranean Archaeolog

Novel IgG-degrading enzymes of the IgdE protease family link substrate specificity to host tropism of <i>Streptococcus</i> species

Recently we have discovered an IgG degrading enzyme of the endemic pig pathogen S. suis designated IgdE that is highly specific for porcine IgG. This protease is the founding member of a novel cysteine protease family assigned C113 in the MEROPS peptidase database. Bioinformatical analyses revealed putative members of the IgdE protease family in eight other Streptococcus species. The genes of the putative IgdE family proteases of S. agalactiae, S. porcinus, S. pseudoporcinus and S. equi subsp. zooepidemicus were cloned for production of recombinant protein into expression vectors. Recombinant proteins of all four IgdE family proteases were proteolytically active against IgG of the respective Streptococcus species hosts, but not against IgG from other tested species or other classes of immunoglobulins, thereby linking the substrate specificity to the known host tropism. The novel IgdE family proteases of S. agalactiae, S. pseudoporcinus and S. equi showed IgG subtype specificity, i.e. IgdE from S. agalactiae and S. pseudoporcinus cleaved human IgG1, while IgdE from S. equi was subtype specific for equine IgG7. Porcine IgG subtype specificities of the IgdE family proteases of S. porcinus and S. pseudoporcinus remain to be determined. Cleavage of porcine IgG by IgdE of S. pseudoporcinus is suggested to be an evolutionary remaining activity reflecting ancestry of the human pathogen to the porcine pathogen S. porcinus. The IgG subtype specificity of bacterial proteases indicates the special importance of these IgG subtypes in counteracting infection or colonization and opportunistic streptococci neutralize such antibodies through expression of IgdE family proteases as putative immune evasion factors. We suggest that IgdE family proteases might be valid vaccine targets against streptococci of both human and veterinary medical concerns and could also be of therapeutic as well as biotechnological use

Archaeology and legislation in Britain

SIGLEAvailable from British Library Document Supply Centre- DSC:95/21835 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

The Still, Peterborough Medieval remains between Cumbergate and Westgate

Available from British Library Document Supply Centre-DSC:1594.736(1) / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Detecting trends in the prediction of the buried past: A review of geophysical techniques in archaeology.

noGeophysical survey techniques are a highly visible part of the scientific toolkit that is now used by archaeologists. In this paper, the history of the use of geophysical techniques in archaeology will be discussed, as will significant research themes associated with the most widely used prospecting devices. It is apparent that while the use of geophysical techniques is at an all-time high, there are many key areas where prospecting is rapidly developing. Some of the advances relate to fundamental aspects of the techniques, while others dictate how we undertake survey in the future. There is a movement away from pre-gridded survey areas towards real-time GPS for navigation. This allows greater integration, or fusion, of disparate data sources using visualization techniques derived from associated disciplines. The analysis of landscapes has become a major component of the application of new technology and there are many challenges to be tackled, including how to analyse and interpret significant archaeology within large-scale, data-rich, multi-technique investigations. The reflective nature of the review acknowledges the important role of Archaeometry in the development of archaeological geophysics