Spatially resolved correlative microscopy and microbial identification reveal dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment.

Coastal salt marshes are key sites of biogeochemical cycling and ideal systems in which to investigate the community structure of complex microbial communities. Here, we clarify structural-functional relationships among microorganisms and their mineralogical environment, revealing previously undescribed metabolic activity patterns and precise spatial arrangements within salt marsh sediment. Following 3.7-day in situ incubations with a non-canonical amino acid that was incorporated into new biomass, samples were resin-embedded and analysed by correlative fluorescence and electron microscopy to map the microscale arrangements of anabolically active and inactive organisms alongside mineral grains. Parallel sediment samples were examined by fluorescence-activated cell sorting and 16S rRNA gene sequencing to link anabolic activity to taxonomic identity. Both approaches demonstrated a rapid decline in the proportion of anabolically active cells with depth into salt marsh sediment, from ~60% in the top centimetre to 9.4%-22.4% between 2 and 10 cm. From the top to the bottom, the most prominent active community members shifted from sulfur cycling phototrophic consortia, to putative sulfate-reducing bacteria likely oxidizing organic compounds, to fermentative lineages. Correlative microscopy revealed more abundant (and more anabolically active) organisms around non-quartz minerals including rutile, orthoclase and plagioclase. Microbe-mineral relationships appear to be dynamic and context-dependent arbiters of biogeochemical cycling.R24 GM137200 - NIGMS NIH HHShttps://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.1566

Controlled Deployment of Gossamer Spacecraft

Deployable gossamer structures for solar sails need to be deployed in a controlled way. Several strategies present have the disadvantage that the sail membrane cannot always be tensioned during the deployment process. In combination with a slow deployment, this involves the risk of an entanglement of the sail. Slow deployments of at least several minutes are desirable in order to keep inertial loads low and to implement Fault-Detection, Fault-Isolation and Recovery Techniques (FDIR). This might further require completely stopping and resuming the deployment process. For gossamer spacecraft based on crossed boom configurations with triangular sail segments, a deployment strategy is described that is assumed to allow such a controlled deployment process. With a combination of folding and coiling, it is ensured that the deployed sail area can be held taut between the partly deployed booms. During deployment, four deployment units with two spools each on which the sail is mounted (a half segment stowed on each) moves away from the central bus unit, the center of the deployed sail. The development was made in the Gossamer-1 project of the German Aerospace Center (DLR). The folding and coiling of the membrane is mathematically modelled. This allows an investigation of the deployment geometry. It provides the mathematical relation between the deployed boom length and the deployed sail membrane geometry. By modelling the coiled zig-zag folding lines it is possible to calculate the deployment force vector as function of the deployment time. The stowing and deployment strategy was verified by tests with an engineering qualification model of the Gossamer-1 deployment unit. According to a test-as-you-fly approach the tests included vibration tests, venting, thermal-vacuum tests and ambient deployment. In these tests the deployment strategy proved to be suitable for a controlled deployment of gossamer spacecraft. A deeper understanding of the deployment process is gained by analyzing the deployment strategy mathematically

Natural and anthropogenic carbon input affect microbial activity in salt marsh sediment

Salt marshes are dynamic, highly productive ecosystems positioned at the interface between terrestrial and marine systems. They are exposed to large quantities of both natural and anthropogenic carbon input, and their diverse sediment-hosted microbial communities play key roles in carbon cycling and remineralization. To better understand the effects of natural and anthropogenic carbon on sediment microbial ecology, several sediment cores were collected from Little Sippewissett Salt Marsh (LSSM) on Cape Cod, MA, USA and incubated with either Spartina alterniflora cordgrass or diesel fuel. Resulting shifts in microbial diversity and activity were assessed via bioorthogonal non-canonical amino acid tagging (BONCAT) combined with fluorescence-activated cell sorting (FACS) and 16S rRNA gene amplicon sequencing. Both Spartina and diesel amendments resulted in initial decreases of microbial diversity as well as clear, community-wide shifts in metabolic activity. Multi-stage degradative frameworks shaped by fermentation were inferred based on anabolically active lineages. In particular, the metabolically versatile Marinifilaceae were prominent under both treatments, as were the sulfate-reducing Desulfovibrionaceae, which may be attributable to their ability to utilize diverse forms of carbon under nutrient limited conditions. By identifying lineages most directly involved in the early stages of carbon processing, we offer potential targets for indicator species to assess ecosystem health and highlight key players for selective promotion of bioremediation or carbon sequestration pathways.Published versio

Gossamer roadmap technology reference study for a solar polar mission

A technology reference study for a solar polar mission is presented. The study uses novel analytical methods to quantify the mission design space including the required sail performance to achieve a given solar polar observation angle within a given timeframe and thus to derive mass allocations for the remaining spacecraft sub-systems, that is excluding the solar sail sub-system. A parametric, bottom-up, system mass budget analysis is then used to establish the required sail technology to deliver a range of science payloads, and to establish where such payloads can be delivered to within a given timeframe. It is found that a solar polar mission requires a solar sail of side-length 100 – 125 m to deliver a ‘sufficient value’ minimum science payload, and that a 2. 5μm sail film substrate is typically required, however the design is much less sensitive to the boom specific mass

Pressure broadening in the 2ν3 band of methane and its implication on atmospheric retrievals

N2-broadened half widths and pressure shifts were obtained for transitions in the 2ν3 methane band. Laboratory measurements recorded at 0.011 cm$^{-1}$ resolution with a Bruker 120 HR Fouriertransform spectrometer were analysed from 5860 to 6185 cm$^{-1}$. A 140 cm gas cell was filled with methane at room temperature and N2 as foreign gas at pressures ranging from 125 to 900 hPa. A multispectrum nonlinear constrained least squares approach based on Optimal Estimation was applied to derive the spectroscopic parameters by simultaneously fitting laboratory spectra at different ambient pressures assuming a Voigt line-shape. At room temperature, the half widths ranged between 0.030 and 0.071 cm$^{-1}$ atm$^{-1}$, and the pressure shifts varied from –0.002 to –0.025 cm$^{-1}$ atm$^{-1}$ for transitions up to J´´=10. Especially for higher rotational levels, we find systematically narrower lines than HITRAN predicts. The Q and R branch of the new set of spectroscopic parameters is further tested with ground based direct sun Fourier transform infrared (FTIR) measurements where systematic fit residuals reduce by about a factor of 3–4. We report the implication of those differences on atmospheric methane measurements using high-resolution ground based FTIR measurements as well as low-resolution spectra from the Scanning Imaging Absorption SpectroMeter for Atmospheric ChartographY (SCIAMACHY) instrument onboard ENVISAT. We find that for SCIAMACHY, a latitudinal and seasonally varying bias of about 1% can be introduced by erroneous broadening parameters

Small Spacecraft in Small Solar System Body Applications

In the wake of the successful Philae landing on comet 67P/Churyumov-Gerasimenko and the launch of the first Mobile Asteroid Surface Scout, MASCOT, aboard the Hayabusa2 space probe to asteroid (162173) Ryugu, small spacecraft in applications related to small solar system bodies have become a topic of increasing interest. Their unique combination of efficient capabilities, resource-friendly design and inherent robustness makes them attractive as a mission element at the frontiers of exploration of the solar system by larger spacecraft as well as stand-alone low-cost approaches to open up the solar system for a broader range of interests. The operators' requirements for cutting-edge missions compatible with available launch capabilities impose significant constraints in resources, timelines, timeliness, mass and size. To create spacecraft feasible within these constraints, the mission design teams need to accept a broad range of equipment maturity levels from fresh concepts to off-the-shelf units. The resulting Constraints-Driven Engineering (CDE) environment has led to new methods which transcend traditional evenly-paced and sequential development. We evolved and extended Concurrent Design and Engineering (CD/CE) methods originally incepted for initial studies into Concurrent Assembly, Integration and Verification (CAIV). It is applied in all phases in most of our projects to achieve convergence of asynchronous subsystem maturity timelines and to match parallel tracks of integration and test campaigns. When facing such a challenge, Model-Based Systems Engineering (MBSE) supports design trades and constant configuration evolution due to unforeseen changes. Proactive change and schedule acceleration has resulted from system-level CD/CE optimization across interface boundaries by MBSE-aided CAIV

Membrane Deployment Technology Development at DLR for Solar Sails and Large-Scale Photovoltaics

Following the highly successful flight of the first interplanetary solar sail, JAXA's IKAROS, with missions in the pipeline such as NASA's NEASCOUT nanospacecraft solar sail and JAXA's Solar Power Sail solar-electric propelled mission to a Jupiter Trojan asteroid, and on the back-ground of the ever increasing power demand of GEO satellites now including all-electric spacecraft, there is renewed interest in large lightweight structures in space. Among these, deployable membrane or 'gossamer' structures can provide very large functional area units for innovative space applications which can be stowed into the limited volumes of launch vehicle fairings as well as secondary payload launch slots, depending on the scale of the mission. Large area structures such as solar sails or high-power photovoltaic generators require a technology that allows their controlled and thereby safe deployment. Before employing such technology for a dedicated science or commercial mission, it is necessary, to demonstrate its reliability, i.e., TRL 6 or higher. A reliable technology that enables controlled deployment was developed in the GOSSAMER-1 solar sail deployment demonstrator project of the German Aerospace Center, DLR, including verification of its functionality with various laboratory tests to qualify the hardware for a first demonstration in low Earth orbit. We provide an overview of the GOSSAMER-1 hardware development and qualification campaign. The design is based on a crossed boom configuration with triangular sail segments. Employing engineering models, all aspects of the deployment were tested under ambient environment. Several components were also subjected to environmental qualification testing. An innovative stowing and deployment strategy for a controlled deployment and the required mechanisms are described. The tests conducted provide insight into the deployment process and allow a mechanical characterization of this process, in particular the measurement of the deployment forces. The stowing and deployment strategy was verified by tests with an engineering qualification model of one out of four GOSSAMER-1 deployment units. According to a test-as-you-fly approach the tests included vibration tests, venting, thermal-vacuum tests and ambient deployment. In these tests the deployment strategy proved to be suitable for a controlled deployment of gossamer spacecraft, and deployment on system level was demonstrated to be robust and controllable. The GOSSAMER-1 solar sail membranes were also equipped with small thin-film photovoltaic arrays intended to supply the core spacecraft. In our follow-on project GOSOLAR, the focus is now entirely on deployment systems for huge thin-film photovoltaic arrays. Based on the GOSSAMER-1 experience, deployment technology and qualification strategies, new technologies for the integration of thin-film photovoltaics are being developed and qualified for a first in-orbit technology demonstration within five years. Main objective is the further development of deployment technology for a 25 m² gossamer solar power generator and a flexible photovoltaic membrane. GOSOLAR enables a wider range of deployment concepts beyond solar sail optimized methods. It uses the S²TEP bus system developed at the Institute of Space Systems as part of the DLR satellite roadmap

Capabilities of Gossamer-1 derived small spacecraft solar sails carrying MASCOT-derived nanolanders for in-situ surveying of NEAs

Any effort which intends to physically interact with specific asteroids requires understanding at least of the composition and multi-scale structure of the surface layers, sometimes also of the interior. Therefore, it is necessary first to characterize each target object sufficiently by a precursor mission to design the mission which then interacts with the object. In small solar system body (SSSB) science missions, this trend towards landing and sample-return missions is most apparent. It also has led to much interest in MASCOT-like landing modules and instrument carriers. They integrate at the instrument level to their mothership and by their size are compatible even with small interplanetary missions. The DLR-ESTEC Gossamer Roadmap NEA Science Working Groups‘ studies identified Multiple NEA Rendezvous (MNR) as one of the space science missions only feasible with solar sail propulsion. Parallel studies of Solar Polar Orbiter (SPO) and Displaced L1 (DL1) space weather early warning missions studies outlined very lightweight sailcraft and the use of separable payload modules for operations close to Earth as well as the ability to access any inclination and a wide range of heliocentric distances. These and many other studies outline the unique capability of solar sails to provide access to all SSSB, at least within the orbit of Jupiter. Since the original MNR study, significant progress has been made to explore the performance envelope of near-term solar sails for multiple NEA rendezvous. However, although it is comparatively easy for solar sails to reach and rendezvous with objects in any inclination and in the complete range of semi-major axis and eccentricity relevant to NEOs and PHOs, it remains notoriously difficult for sailcraft to interact physically with a SSSB target object as e.g. the Hayabusa missions do. The German Aerospace Center, DLR, recently brought the Gossamer solar sail deployment technology to qualification status in the Gossamer-1 project. Development of closely related technologies is continued for very large deployable membrane-based photovoltaic arrays in the GoSolAr project. We expand the philosophy of the Gossamer solar sail concept of efficient multiple sub-spacecraft integration to also include landers for one-way in-situ investigations and sample-return missions. These are equally useful for planetary defence scenarios, SSSB science and NEO utilization. We outline the technological concept used to complete such missions and the synergetic integration and operation of sail and lander. We similarly extend the philosophy of MASCOT and use its characteristic features as well as the concept of Constraints-Driven Engineering for a wider range of operations

Small Spacecraft Based Multiple Near-Earth Asteroid Rendezvous and Landing with Near-Term Solar Sails and ‘Now-Term‘ Technologies

Physical interaction with small solar system bodies (SSSB) is the next step in planetary science, planetary in-situ resource utilization (ISRU), and planetary defense (PD). It requires a broader understanding of the surface properties of the target objects, with particular interest focused on those near Earth. Knowledge of composition, multi-scale surface structure, thermal response, and interior structure is required to design, validate and operate missions addressing these three fields. The current level of understanding is occasionally simplified into the phrase, ”If you’ve seen one asteroid, you’ve seen one asteroid”, meaning that the in-situ characterization of SSSBs has yet to cross the threshold towards a robust and stable scheme of classification. This would enable generic features in spacecraft design, particularly for ISRU and science missions. Currently, it is necessary to characterize any potential target object sufficiently by a dedicated pre-cursor mission to design the mission which then interacts with the object in a complex fashion. To open up strategic approaches, much broader in-depth characterization of potential target objects would be highly desirable. In SSSB science missions, MASCOT-like nano-landers and instrument carriers which integrate at the instrument level to their mothership have met interest. By its size, MASCOT is compatible with small interplanetary missions. The DLR-ESTEC Gossamer Roadmap Science Working Groups‘ studies identified Multiple Near-Earth asteroid (NEA) Rendezvous (MNR) as one of the space science missions only feasible with solar sail propulsion. The Solar Polar Orbiter (SPO) study showed the ability to access any inclination, theDisplaced-L1 (DL1) mission operates close to Earth, where objects of interest to PD and for ISRU reside. Other studies outline the unique capability of solar sails to provide access to all SSSB, at least within the orbit of Jupiter, and significant progress has been made to explore the performance envelope of near-term solar sails for MNR. However, it is difficult for sailcraft to interact physically with a SSSB. We expand and extend the philosophy of the recently qualified DLR Gossamer solar sail deployment technology using efficient multiple sub-spacecraft integration to also include landers for one-way in-situ investigations and sample-return missions by synergetic integration and operation of sail and lander. The MASCOT design concept and its characteristic features have created an ideal counterpart for thisand has already been adapted to the needs of the AIM spacecraft, former part of the NASA-ESA AIDA mission. Designing the combined spacecraft for piggy-back launch accommodation enables low-cost massively parallel access to the NEA population

Solar Sails for Planetary Defense and High-Energy Missions

20 years after the successful ground deployment test of a (20 m)² solar sail at DLR Cologne, and in the light of the upcoming U.S. NEAscout mission, we provide an overview of the progress made since in our mission and hardware design studies as well as the hardware built in the course of our solar sail technology development. We outline the most likely and most efficient routes to develop solar sails for useful missions in science and applications, based on our developed ‘now-term’ and near-term hardware as well as the many practical and managerial lessons learned from the DLR-ESTEC GOSSAMER Roadmap. Mission types directly applicable to planetary defense include single and Multiple NEA Rendezvous ((M)NR) for precursor, monitoring and follow-up scenarios as well as sail-propelled head-on retrograde kinetic impactors (RKI) for mitigation. Other mission types such as the Displaced L1 (DL1) space weather advance warning and monitoring or Solar Polar Orbiter (SPO) types demonstrate the capability of near-term solar sails to achieve asteroid rendezvous in any kind of orbit, from Earth-coorbital to extremely inclined and even retrograde orbits. Some of these mission types such as SPO, (M)NR and RKI include separable payloads. For one-way access to the asteroid surface, nanolanders like MASCOT are an ideal match for solar sails in micro-spacecraft format, i.e. in launch configurations compatible with ESPA and ASAP secondary payload platforms. Larger landers similar to the JAXA-DLR study of a Jupiter Trojan asteroid lander for the OKEANOS mission can shuttle from the sail to the asteroids visited and enable multiple NEA sample-return missions. The high impact velocities and re-try capability achieved by the RKI mission type on a final orbit identical to the target asteroid‘s but retrograde to its motion enables small spacecraft size impactors to carry sufficient kinetic energy for deflection