Gender and Inclusion Toolbox: Participatory Research in Climate Change and Agriculture

This manual is a resource and toolbox for NGO practitioners and programme designers interested in diagnostic and action research for gender sensitive and socially inclusive climate change programmes in the rural development context. It is meant to be an easy to use manual, increasing the research capacity, skills and knowledge of its users. Integrating gender and social differentiation frameworks should ideally begin from the start of the programme cycle and be coordinated throughout research, design, implementation, and monitoring and evaluation phases. The data gathered using this toolbox supports this programme work. While the manual emphasizes participatory and qualitative approaches, many of the activities and tools can produce quantitative data. Each chapter features a bundle of research tools intended to be used sequentially. However, we know that each organization has its diverse needs. The chapters are in modular format so that teams can assemble their own research toolbox specific to their needs

Caja de herramientas para género e inclusión: Investigación participativa en cambio climático y agricultura

El presente manual es una caja de herramientas y recursos para profesionales de ONG y diseñadores de programas interesados en el diagnóstico y la investigación-acción sobre programas relacionados con el cambio climático socialmente incluyentes y sensibles al género, en el contexto del desarrollo rural. El manual busca ser de fácil uso, con el fin de mejorar la capacidad de investigación, las habilidades y el conocimiento de quienes lo usan. Idealmente, la integración de los marcos de diferenciación social y de género debería hacerse desde el inicio del programa y coordinarse a lo largo de las fases de investigación, diseño, implementación, monitoreo y evaluación. La información que se puede recolectar utilizando esta caja de herramientas apoya, precisamente, la ejecución de estas fases. Aunque el manual hace énfasis en enfoques participativos y cualitativos, muchas de las actividades y herramientas pueden generar información cuantitativa. En cada capítulo se ofrece una serie de herramientas para la investigación diseñadas para ser utilizadas en forma secuencial. Sin embargo, estamos conscientes de que cada organización tiene diferentes necesidades de información. Los capítulos se presentan en forma de módulos para que los equipos de trabajo puedan armar diferentes cajas de herramientas según sus necesidades de investigación

Author Correction: Measuring light scattering and absorption in corals with Inverse Spectroscopic Optical Coherence Tomography (ISOCT): a new tool for non-invasive monitoring.

An amendment to this paper has been published and can be accessed via a link at the top of the paper

CA125/MUC16 Is Dispensable for Mouse Development and Reproduction

Cancer antigen 125 (CA125) is a blood biomarker that is routinely used to monitor the progression of human epithelial ovarian cancer (EOC) and is encoded by MUC16, a member of the mucin gene family. The biological function of CA125/MUC16 and its potential role in EOC are poorly understood. Here we report the targeted disruption of the of the Muc16 gene in the mouse. To generate Muc16 knockout mice, 6.0 kb was deleted that included the majority of exon 3 and a portion of intron 3 and replaced with a lacZ reporter cassette. Loss of Muc16 protein expression suggests that Muc16 homozygous mutant mice are null mutants. Muc16 homozygous mutant mice are viable, fertile, and develop normally. Histological analysis shows that Muc16 homozygous mutant tissues are normal. By the age of 1 year, Muc16 homozygous mutant mice appear normal. Downregulation of transcripts from another mucin gene (Muc1) was detected in the Muc16 homozygous mutant uterus. Lack of any prominent abnormal phenotype in these Muc16 knockout mice suggests that CA125/MUC16 is not required for normal development or reproduction. These knockout mice provide a unique platform for future studies to identify the role of CA125/MUC16 in organ homeostasis and ovarian cancer

Two spatiotemporally distinct value systems shape reward-based learning in the human brain

Avoiding repeated mistakes and learning to reinforce rewarding decisions is critical for human survival and adaptive actions. Yet, the neural underpinnings of the value systems that encode different decision-outcomes remain elusive. Here coupling single-trial electroencephalography with simultaneously acquired functional magnetic resonance imaging, we uncover the spatiotemporal dynamics of two separate but interacting value systems encoding decision-outcomes. Consistent with a role in regulating alertness and switching behaviours, an early system is activated only by negative outcomes and engages arousal-related and motor-preparatory brain structures. Consistent with a role in reward-based learning, a later system differentially suppresses or activates regions of the human reward network in response to negative and positive outcomes, respectively. Following negative outcomes, the early system interacts and downregulates the late system, through a thalamic interaction with the ventral striatum. Critically, the strength of this coupling predicts participants’ switching behaviour and avoidance learning, directly implicating the thalamostriatal pathway in reward-based learning

Prevalence of mental disorders from adolescence through early adulthood in American Indian and First Nations communities

Indigenous communities lack representation in psychiatric epidemiology despite disproportionate exposure to risk factors. We document the cumulative and 12-month prevalence of psychiatric disorders across the early life course among a sample of Indigenous young adults and compare prospective and retrospective reporting of lifetime mental disorders. This community-based participatory research includes data from 735 Indigenous people from 8 reservations/reserves. Personal interviews were conducted between 2002–2010 and 2017–2018 totaling 9 waves; diagnostic assessments of DSM-IV-TR alcohol abuse/dependence, marijuana use/dependence, other substance abuse/dependence, generalized anxiety disorder, major depressive disorder, dysthymic disorder, and attention deficit/hyperactivity disorder occurred at waves 1 (mean age = 11.1 years), 4 (mean age = 14.3 years), 6 (mean age = 16.2 years), 8 (mean age = 18.3 years), and 9 (mean age = 26.3 years). Cumulative lifetime psychiatric disorders reached 77.3% and lifetime comorbidity 56.4% by wave 9. Past-year prevalence and comorbidity at wave 9 were 28.7% and 6.7%, respectively. Substance use disorders (SUDs) were most common with peak past-year prevalence observed when participants were on average 16.3 years old then declining thereafter. Trends in early life course psychiatric disorders in this study with Indigenous participants highlight cultural variations in psychiatric epidemiology including surprisingly low rates of internalizing disorders in the face of risk factors, disproportionately high rates of early-onset and lifetime SUD, and lower rates of past-year SUD in early adulthood compared with prior research.Peer reviewedSociolog