A directed mutation operator for real coded genetic algorithms

Copyright @ Springer-Verlag Berlin Heidelberg 2010.Developing directed mutation methods has been an interesting research topic to improve the performance of genetic algorithms (GAs) for function optimization. This paper introduces a directed mutation (DM) operator for GAs to explore promising areas in the search space. In this DM method, the statistics information regarding the fitness and distribution of individuals over intervals of each dimension is calculated according to the current population and is used to guide the mutation of an individual toward the neighboring interval that has the best statistics result in each dimension. Experiments are carried out to compare the proposed DM technique with an existing directed variation on a set of benchmark test problems. The experimental results show that the proposed DM operator achieves a better performance than the directed variation on most test problems

C to U editing at position 32 of the anticodon loop precedes tRNA 5′ leader removal in trypanosomatids

In all organisms, precursor tRNAs are processed into mature functional units by post-transcriptional changes. These involve 5′ and 3′ end trimming as well as the addition of a significant number of chemical modifications, including RNA editing. The only known example of non-organellar C to U editing of tRNAs occurs in trypanosomatids. In this system, editing at position 32 of the anticodon loop of tRNAThr(AGU) stimulates, but is not required for, the subsequent formation of inosine at position 34. In the present work, we expand the number of C to U edited tRNAs to include all the threonyl tRNA isoacceptors. Notably, the absence of a naturally encoded adenosine, at position 34, in two of these isoacceptors demonstrates that A to I is not required for C to U editing. We also show that C to U editing is a nuclear event while A to I is cytoplasmic, where C to U editing at position 32 occurs in the precursor tRNA prior to 5′ leader removal. Our data supports the view that C to U editing is more widespread than previously thought and is part of a stepwise process in the maturation of tRNAs in these organisms

In vitro evaluation of dentinal tubule penetration and biomineralization ability of a new root-end filling material

Introduction: Capasio is being developed as a new generation of endodontic material with potential use as a root-end filling material. The aim of this study was to compare the ability of Capasio and mineral trioxide aggregate (MTA) to penetrate human dentinal tubules and examine the interaction of Capasio and MTA with a synthetic tissue fluid (STF) and root canal walls in extracted human teeth. Methods: Root-end preparations were filled with Capasio or MTA, allowed to set for 4 weeks in STF, and then sectioned at 1, 2, and 3 mm from resected surface. Depth of penetration was evaluated by using scanning electron microscopy (SEM). Next, Capasio and MTA samples were prepared both in 1-g pellets and in root-end preparations. Samples were placed in STF, allowed to set, and then characterized by using SEM, energy dispersive x-ray analysis (EDXA), and x-ray diffraction (XRD) techniques. Results: Penetration of Capasio into dentinal tubules was observed at all levels. No penetration of MTA into dentinal tubules was observed at any level. Both Capasio and MTA formed apatite crystals in the supernatant, on their exposed surfaces, and in the interfacial layers that were similar in structure and elemental composition when evaluated by using SEM and EDXA. XRD analysis of these crystals corresponds with those reported for hydroxyapatite. Conclusions: When used as a root-end filling material, Capasio is more likely to penetrate dentinal tubules. Both Capasio and MTA promote apatite deposition when exposed to STF

Properties of Nucleon Resonances by means of a Genetic Algorithm

We present an optimization scheme that employs a Genetic Algorithm (GA) to determine the properties of low-lying nucleon excitations within a realistic photo-pion production model based upon an effective Lagrangian. We show that with this modern optimization technique it is possible to reliably assess the parameters of the resonances and the associated error bars as well as to identify weaknesses in the models. To illustrate the problems the optimization process may encounter, we provide results obtained for the nucleon resonances $\Delta$(1230) and $\Delta$(1700). The former can be easily isolated and thus has been studied in depth, while the latter is not as well known experimentally.Comment: 12 pages, 10 figures, 3 tables. Minor correction

Extrauterine listeriosis in the gravid mouse influences embryonic growth and development

Gravid mice and other rodents inoculated with Listeria monocytogenes typically fail to clear an intrauterine infection and either succumb or expel their intrauterine contents. We took advantage of this property to investigate the effects of an extrauterine infection on parameters of pregnancy success. Pregnant mice were selected for our study if they showed no clinical signs of listeriosis following oral inoculation at 7.5 gestational days (gd), and had no detectable intrauterine colony forming units (cfu) at near term (18.5 gd). The range of oral doses employed was 10(6)-10(8) cfu per mouse for two listerial serotype strains (4nonb and 1/2a). At all doses, inoculation resulted in a decrease in average near-term (18.5 gd) fetal weight per litter compared to sham inoculated controls. Additionally, embryonic death (indicated by intrauterine resorptions) was exhibited by some inoculated mice but was absent in all sham inoculated animals. In parallel experiments designed to detect possible loss of placental function, gravid uteruses were examined histopathologically and microbiologically 96 h after oral inoculation. Placental lesions were associated with high (> 10(6)), but not low (< 10(2)) or absent intrauterine cfu. In vitro, mouse embryonic trophoblasts were indistinguishable from mouse enterocytes in terms of their sensitivity to listerial exposure. A model consistent with our observations is one in which products (host or bacterial) generated during an acute infection enter embryos transplacentally and influences embryonic survival and slows normal growth in utero

When non-activists care: group efficacy mediates the effect of social identification and perceived instability on the legitimacy of collective action

In recent years, multiple social movements have emerged around the world. In addition, public surveys indicate the highest recorded levels of support for protest. In this context of acceptance of collective action, we examine the role of non-activists in the legitimacy of social movements, as this ‘passive’ support can contribute to social change. Given that antecedents of legitimacy have been neglected in the literature, we carried out a survey (N = 605) among a general sample of the population in Chile to shed light on this issue. We found that social identification with movements and perceived instability predicted the perceived legitimacy of protests by social movements, and that both variables had only indirect effects, through group efficacy. This suggests that perceiving social movements as able to achieve success can lead non-activists to perceive their actions as legitimate, highlighting the importance to movements of being seen to be effective

Testing many treatments within a single protocol over 10 years at MRC Clinical Trials Unit at UCL: Multi-arm, multi-stage platform, umbrella and basket protocols

There is real need to change how we do some of our clinical trials, as currently the testing and development process is too slow, too costly and too failure-prone often we find that a new treatment is no better than the current standard. Much of the focus on the development and testing pathway has been in improving the design of phase I and II trials. In this article, we present examples of new methods for improving the design of phase III trials (and the necessary lead up to them) as they are the most time-consuming and expensive part of the pathway. Key to all these methods is the aim to test many treatments and/or pose many therapeutic questions within one protocol

High Order Multistep Methods with Improved Phase-Lag Characteristics for the Integration of the Schr\"odinger Equation

In this work we introduce a new family of twelve-step linear multistep methods for the integration of the Schr\"odinger equation. The new methods are constructed by adopting a new methodology which improves the phase lag characteristics by vanishing both the phase lag function and its first derivatives at a specific frequency. This results in decreasing the sensitivity of the integration method on the estimated frequency of the problem. The efficiency of the new family of methods is proved via error analysis and numerical applications.Comment: 36 pages, 6 figure