Creating a Research Footprint: Introducing an Institutional Repository to Scholars

Touro Scholar, the institutional repository (IR) of the Touro College and University System (TCUS) and New York Medical College (NYMC), officially launched in April 2016, utilizing bepress’s Digital Commons repository platform.The IR is an online archive of scholarly output of an institution –in this case TCUS and NYMC. After assessing institutional knowledge of the scholarly benefits of depositing work in the repository, the libraries planned to create a concrete game plan to establish buy-in to Touro Scholar. Handouts, presentations, customized guides, liaison outreach, tutorials, and webinars were proposed methods of advertising the repository

Использование фильтровальной установки для очистки водопроводной воды от солей жёсткости

Очистка воды в домашних условиях имеет очень важное значение. В статье проведено исследование ионообменной способности фильтровального модуля при извлечении солей жёсткости из водопроводной воды. Сделан вывод об эффективности извлечения солей жёсткости исследуемым фильтром в сравнении с ранее тестируемыми фильтровальными системами. Water purification in home conditions is very important. In this article, the ion-exchange characteristics of filtering module were studied over extraction of hardness salts from tap water. A conclusion is drawn about efficiency of hardness salts extraction by the investigated filter in comparison with the previously tested filter systems

Разработка генератора холодной плазмы для нуклеопластики

В настоящей работе мы исследовали низкотемпературную плазму и ее параметры для разработки генератора холодной плазмы для нуклеопластики. На первом этапе нашей исследовательской работы мы изучили более 20-ти патентов на приборы, основной функцией которых являлось образование холодной плазмы в электролитах. Мы выявили наиболее приемлемые параметры разрабатываемого нами генератора, а также следующие особенности: 1.Плазма формируется в электролите. 2.Для нуклеопластики используется малый размер электрода. 3.Необходимость возбуждения и удержания режима плазмы. 4.Для режима плазмы требуется меньше мощность, чем для нагрева проводящей ткани от прохождения переменного тока высокой частоты

Structural characterization of the closed conformation of mouse guanylate kinase

Guanylate kinase (GMPK) is a nucleoside monophosphate kinase that catalyzes the reversible phosphoryl transfer from ATP to GMP to yield ADP and GDP. In addition to phosphorylating GMP, antiviral prodrugs such as acyclovir, ganciclovir, and carbovir and anticancer prodrugs such as the thiopurines are dependent on GMPK for their activation. Hence, structural information on mammalian GMPK could play a role in the design of improved antiviral and antineoplastic agents. Here we present the structure of the mouse enzyme in an abortive complex with the nucleotides ADP and GMP, refined at 2.1 Angstrom resolution with a final crystallographic R factor of 0.19 (R-free = 0.23). Guanylate kinase is a member of the nucleoside monophosphate (NMP) kinase family, a family of enzymes that despite having a low primary structure identity share a similar fold, which consists of three structurally distinct regions termed the CORE, LID, and NMP-binding regions. Previous studies on the yeast enzyme have shown that these parts move as rigid bodies upon substrate binding. It has been proposed that consecutive binding of substrates leads to "closing" of the active site bringing the NMP-binding and LID regions closer to each other and to the CORE region. Our structure, which is the first of any guanylate kinase with both substrates bound, supports this hypothesis. It also reveals the binding site of ATP and implicates arginines 44, 137, and 148 (in addition to the invariant P-loop lysine) as candidates for catalyzing the chemical step of the phosphoryl transfer

Maximum illumination control system for photovoltaic panels orientation

The article describes the solar tracker for photovoltaic panels and energy systems based on such devices. The authors introduce the results of calculations of the solar tracker application effectiveness for solar energy systems and the results of the field testing in Tomsk

Development and Application of Droplet Digital PCR Tools for the Detection of Transgenes in Pastures and Pasture-Based Products

Implementation of molecular biotechnology, such as transgenic technologies, in forage species can improve agricultural profitability through achievement of higher productivity, better use of resources such as soil nutrients, water, or light, and reduced environmental impact. Development of detection and quantification techniques for genetically modified plants are necessary to comply with traceability and labeling requirements prior to regulatory approval for release. Real-time PCR has been the standard method used for detection and quantification of genetically modified events, and droplet digital PCR is a recent alternative technology that offers a higher accuracy. Evaluation of both technologies was performed using a transgenic high-energy forage grass as a case study. Two methods for detection and quantification of the transgenic cassette, containing modified fructan biosynthesis genes, and a selectable marker gene, hygromycin B phosphotransferase used for transformation, were developed. Real-time PCR was assessed using two detection techniques, SYBR Green I and fluorescent probe-based methods. A range of different agricultural commodities were tested including fresh leaves, tillers, seeds, pollen, silage and hay, simulating a broad range of processed agricultural commodities that are relevant in the commercial use of genetically modified pastures. The real-time and droplet digital PCR methods were able to detect both exogenous constructs in all agricultural products. However, a higher sensitivity and repeatability in transgene detection was observed with the droplet digital PCR technology. Taking these results more broadly, it can be concluded that the droplet digital PCR technology provides the necessary resolution for quantitative analysis and detection, allowing absolute quantification of the target sequence at the required limits of detection across all jurisdictions globally. The information presented here provides guidance and resources for pasture-based biotechnology applications that are required to comply with traceability requirements

SNP Discovery and Haplotypic Variation in Full-Length Herbage Quality Genes of Perennial Ryegrass (Lolium Perenne L.)

The development of forages with enhanced nutritive value through improvements of herbage quality (digestibility, carbohydrate content) is potentially capable of increasing both meat and milk production by up to 25%. However, the expense and time-consuming nature of the relevant biochemical and biophysical assays has limited breeding improvement for forage quality. The development of accurate high-throughput molecular marker-based selection systems such as single nucleotide polymorphisms (SNPs) permits evaluation of genetic variation and selection of favourable variants to accelerate the production of elite new varieties