Flash-spectroscopic and electrophysiological investigation of excised retinae from the dark adapted bos taurus domesticus

1.) A flash spectrophotometer adapted to the simultaneous recording of spectroscopic and electrophysiological parameters from excised bovine retinae was developed, with a maximum time resolution of 2 $\mu$s and an amplitude resolution of 2x10$^{-4}$. 2.) All experiments were performed with dark adapted eyesfrom blindfolded animals to avoid problems related to different light adaptation and to rnaxirnize their content of unbleached rhodopsin. 3.) A new preparation method is described, which results in selected parts of bovine retinae for spectroscopical and electrophysiological investigations. The method is gentle enough to keep the sampies in a condition which allowed measurements of the late receptor potential for more than seven hours. 4.) The latency of the a-wave was determined to be notgreater than 1.3 ms at 37$^\circ$ C. 5.) Records of the formation and decay of MI had small amplitudes and did not allow a detailed kinetic analysis. The half-times of the MI-decay between 10$^\circ$ C and 30$^\circ$ C fit into the time range of the MII-formation. From the temperature-dependent shift of the isosbestie pointbetween MII and rhodopsin it is concluded that an equilibriurn between MI and MII does exist for excised retinae. 6.) The formation of MII was traced at two different wavelengths about 120 nm apart. At both wavelengths the signals show the same first order reaction; at 378 nm an additional, faster, component is detected. Theformation of MII at 378 nm can be described by a sum of two first order reactions. The origin of this difference between the 378 nm signal and the one at 506 nm is not known. 7.) For 37$^\circ$ C a direct correlation between the R2 component of the early receptor potential and the formation of MII was found. The R2-response could be matched by a difference of two weighted exponentials, whose time constants were identical to those obtained for the MII-formation measured at 378 nm.8.) The recorded signals were digitally processed by routines especially programmed by the author for this purpose. The data could be subjected to digital lowpassfiltering, computation of their power density spectra, and determination of time constants if they represent single first order reactions

Data for: Comparative Transcriptomics and Genomic Patterns of Discordance in Capsiceae (Solanaceae)

Here we provide 6515 gene alignments for species of Capsicum and Lycianthes. These alignments include Bayes blocks, indicating their best fit model of molecular evolution (BIC criterion). These files are ready to run in MrBayes

Forschung in Nordrhein-Westfalen

Available from TIB Hannover: F95B1418 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman