Galectin-1 protein modified gold (III)-PEGylated complex-nanoparticles: Proof of concept of alternative probe in colorimetric glucose detection

International audienc

Pegylated doxorubicin gold complex: From nanovector to potential intercalant agent for biosensor applications

We report an original approach to synthesize hybrid gold nanostructures in which doxorubicin (DOX), mixed to Poliethylenglycole diacid (PEG-COOH) led to original hybrid gold nanovector (DOX IN PEG AuNPs). In this work, we investigate the ability of DOX IN PEG-AuNPs to detect the amplification of the hybridization process by a sensitive Quartz crystal Microbalance with dissipation (QCM-D) by intercalation process. The sensing layer was carried out by self-assembled monolayer of β mercaptoethylamine (cysteamine) on gold-coated quartz crystal sensor composed by a rigid homobifunctional cross-linker 1,4 phenilenediisothiocyanate (PDITC) linked covalently with amino-probe oligonucleotides. By QCM characterization in the range from 8 µM to 20 nM, we demonstrate high specificity of DOX IN PEG-AuNPs-DNA with a limit of detection (LOD) of 9 nM. This result is very promising for development of sensitive and effective nanoparticle-based biosensor for quantifying small biomolecules concentration in physiological liquids. These results open a possibility to realize a new class of nanovector which will be tailored for different biomedical application, such as imaging, targeting and drugs delivery. Keywords: Quartz Crystal Microbalance (QCM), Chemical surface, DNA hybridization, Gold nanoparticles, Doxorubici

Endemic Plants: From Design to a New Way of Smart Hybrid Nanomaterials for Green Nanomedicine Applications

International audienc

Plasmonic DNA: Towards Genetic Diagnosis Chips

"Plasmonics and DNA" special issueInternational audienc

Biodegradable polymeric nanomaterials

International audienc

Novel Synthesis and Characterization of Doxycycline-Loaded Gold Nanoparticles: The Golden Doxycycline for Antibacterial Applications

International audienc

Aptamer Grafting onto (on) and into (in) Pegylated Gold Nanoparticles: Physicochemical Characterization and In vitro Cytotoxicity Investigation in Renal Cells

International audienceGold Nanoparticles (AuNPs) have already a remarkable interest as viable biomedical materials. Additionally, the strategy of using biomolecules to modify their surface properties is a very attractive as it leads to the generation of new nanometric hybrid materials. In this respect, aptamers, functional small single-strand oligonucleotides (DNA or RNA), are ideal candidates for molecular targeting applications since the high affinity to their target molecules. Thus, the urge of new and effective methodologies to graft aptamers on AuNPs is rapidly increasing especially for applications in bioanalysis and biomedicine, including early diagnosis and drug delivery. Here we used two chemical methodologies to conjugate the aptamer (APT) onto pegylated gold nanoparticles (PEG-AuNPs): the carbodiimide chemistry (EDC/NHS) (methodology ON) and the chelation-bond (R-Au bond) (methodology IN). The aptamer's conjugations with the PEG-AuNPs were characterized by UV-Vis absorption, Raman Spectroscopy and transmission electron microscopy (TEM). In addition, the potential nanotoxicity of the two aptamer-conjugated AuNPs was evaluated on two different renal cell lines, being the kidneys one of the most important site of bioaccumulation upon systemic circulation. Interestingly, the two aptamer-conjugated AuNPs showed different cytotoxicity when exposed to human embryonic kidney (HEK293) and mouse collecting duct cells (M-1), indicating that cell viability has to be taken into account when choosing the proper strategy for NPs production. In conclusion this study provides two effective methods to graft aptamers on NPs and important insights regarding NPs conformation and the relative cell viability

Cell penetrating peptide (CPP) gold( iii ) – complex – bioconjugates: from chemical design to interaction with cancer cells for nanomedicine applications

International audienceInnovative synthesis of a nanotheragnostic scaffold capable of targeting and destroying pancreatic cancer cells (PDAC) using the Biotinylated NFL-TBS.40-63 peptide (BIOT-NFL)

Polyethylene glycol Gold-Nanoparticles: Facile Nanostructuration of Doxorubicin and its complex with DNA molecules for SERS detection

International audienceWe report the synthesis of dicarboxylic acid-terminated polyethylene-glycol (PEG)-gold nanoparticles by a simple one-step method, and their further use to form nanostructured surfaces for biomolecule immobilization. The synthesized nano-scale particles were conjugated with probe/target oligonucleotides in order to evaluate intercalation phenomenon in the presence of doxorubicin drug via Surface Enhanced Raman Spectroscopy (SERS) analysis