254 research outputs found
Intermittent Small Baseline Subset (ISBAS) monitoring of land covers unfavourable for conventional C-band InSAR: proof-of-concept for peatland environments in North Wales, UK
This paper provides a proof-of-concept for the use of the new Intermittent Small Baseline Subset (ISBAS) approach to study ground elevation changes in areas of peat and organic soils in north Wales, which are generally, unfavourable for conventional C-band interferometric applications. A stack of 53 ERS-1/2 C-band SAR scenes acquired between 1993 and 2000 in descending mode was processed with both the standard low-pass SBAS method and ISBAS. The latter revealed exceptional improvements in the coverage of ground motion solutions with respect to the standard approach. The number of identified coherent and intermittently coherent pixels increased by a factor of 26 with respect to the SBAS solution, and extended the coverage of results across unfavourable land covers, particularly for coniferous woodland, bog, acid grassland and heather. The greatest increase was achieved over coniferous woodland, which showed ISBAS/SBAS pixel density ratios above 300. Despite the intermittent nature of the ISBAS solutions, ISBAS provided velocity standard errors generally below 1-1.5 mm/yr, thus preserving good quality of the estimated ground motion rates
Intermittent SBAS (ISBAS) InSAR with COSMO-SkyMed X-band high resolution SAR data for landslide inventory mapping in Piana degli Albanesi (Italy)
In the context of recent advances in InSAR processing techniques to retrieve higher persistent scatterer and coherent target densities over unfavourable land cover classes, this study tests the Intermittent Small Baseline Subset (ISBAS) approach to update the landslide inventory around the town of Piana degli Albanesi (Italy), an area where only 2% of the land appears suitable to generate radar scatterers based on a pre-survey feasibility assessment. ISBAS processing of 38 ascending mode and 36 descending mode COSMO-SkyMed StripMap HIMAGE SAR scenes at 3m resolution allows identification of ~726,000 and ~893,000 coherent and intermittently coherent pixels for the ascending and descending data stacks respectively. Observed improvements in the number of ISBAS solutions for the ascending mode are greater than 40 times compared to the conventional SBAS approach, not only for urban and rocky terrains, but also rural and vegetated land covers. Line of sight ground motion rates range between -6.4 and +5.5 mm/yr in 2008-2011, although the majority of the processed area shows general stability, with average rates of -0.6 mm/yr in the ascending and -0.1 mm/yr in the descending mode results. Interpretation of the ISBAS deformation rates, integrated with targeted field surveys and aerial photo-interpretation, provides a new and more complete picture of landslide distribution, state of activity and intensity in the test area, and allows depiction of very slow and extremely slow landslide processes even in areas difficult to access, with unprecedented coverage of results. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only
Monitoring tropical peat related settlement using ISBAS InSAR, Kuala Lumpur International Airport (KLIA)
Rapid population growth in South-East Asia has placed immense pressure upon lowland regions both to supply food and employment and space for residential, commercial and infrastructure development. This pressure has led to sites on tropical peatland previously considered unsuitable for development to be revisited. One such site, the KLIA2 terminal and runway, Kuala Lumpur International Airport which opened in May 2014 at a cost of 3.6 billion MYR has been beset by well documented subsidence problems. Coverage of the tropics by the Sentinel-1 satellite constellation presents an opportunity to monitor the ongoing subsidence at KLIA 2, identify potential knowledge gaps and help inform more sustainable infrastructure development in tropical peatland regions. Our results show that the ISBAS InSAR method produces reproducible ground deformation maps which can clearly identify the patterns of deformation across both urban infrastructure and adjacent rural plantations and tropical peat swamp. This is particularly well defined around the terminal building at KLIA-2 where different ground preparation and foundation design have resulted in stable terminal buildings and subsidence of surrounding pavement. Deformation is greatest in the runway area where alternate bands of uplift and subsidence presumably accompany the greatest loads associated with landing aircraft. In contrast, areas where peat replacement was the primary form of ground preparation, ground motion is stable, however this comes at high economic and environmental cost
Monitoring rail infrastructure using multisensor navigation on a moving platform and autonomous robots
RailSat aims to use Global Navigation Satellite System (GNSS) to monitor and maintain railway assets and its surrounding environment by railway asset owners and/or other relevant stakeholders.
The rail sector is looking for continuous monitoring solutions which have no impact on the train service, both wayside (track bound) and onboard (train bound), which require accurate positioning while travelling at high speeds (>120kmh).
This paper focuses on the combination of positioning data from traditional GNSS/INS system with processed LIDAR point cloud and discusses real-life results from the Snake Pass, Peak District, England. Data have been collected using a dedicated multisensory van but the nature of the road allows us to draw conclusions relevant to the rail industry.
This paper discusses the proposed deployment of a mobile LiDAR monitoring system consisting of a set of laser scanners and a navigation component. While the LIDAR component is capable of centimetre accuracy, it is limited by the navigation accuracy, predominantly affected by the difficult railway environment, frequent multipath and NLOS interference combined with a loss of signal next to the monitoring structures itself (bridges, cuttings, tunnels, embankments etc.), making precise positioning the biggest challenge.
The proposed navigation system combines IMU positioning system with a computer vision system capable of localisation using features in the natural environment. This paper outlines the combination of the proposed navigation system with the LIDARâs information, which provides two ways of correcting navigation trajectory in post-processing
Use of Surface Motion Characteristics Determined by InSAR to Assess Peatland Condition
Peatland surface motion is a key property of peatland that relates to condition. However, fieldâbased techniques to measure surface motion are not costâeffective over large areas and long time periods. An alternative method that can quantify peatland surface motion over large areas is interferometric synthetic aperture radar. Although field validation of the accuracy of this method is difficult, the value of InSAR as a means of quantifying peat condition can be tested. To achieve this the characteristics of InSAR time series measured over an18âmonth period at 22 peatland sites in the Flow Country northern Scotland were compared to site condition assessment based on plant functional type and site management history. Sites in good condition dominated by Sphagnum display longâterm stability or growth and a seasonal cycle with maximum uplift and subsidence in AugâNov and AprilâJune respectively. Drier and partially drained sites dominated by shrubs display longâterm subsidence with maximum uplift and subsidence in JulyâOct and FebâJune respectively. Heavily degraded sites with large bare peat extent display subsidence with no distinct seasonal oscillations. Seasonal oscillation in surface motion at sites with a dominant nonâvascular plant community is interpreted as resulting from changes in seasonal evaporative demand. On sites with extensive vascular plants cover and falling water table, surface oscillations are interpreted as representing sustained drawdown during the growing season and subsequent recharge in late winter. This study highlights the potential to use InSAR to characterize peatland condition and provide a new view of the surface dynamics of peatland landscapes
Identification of typical ecohydrological behaviours using InSAR allows landscape-scale mapping of peatland condition
Better tools for rapid and reliable assessment of global peatland extent and condition are urgently needed to support action to prevent further decline of peatlands. Peatland surface motion is a response to changes in the water and gas content of a peat body regulated by the ecology and hydrology of a peatland system. Surface motion is therefore a sensitive measure of ecohydrological condition but has traditionally been impossible to measure at the landscape scale. Here we examine the potential of surface motion metrics derived from satellite interferometric synthetic aperture radar (InSAR) to map peatland condition in a blanket bog landscape. We show that the timing of maximum seasonal swelling of the peat is characterised by a bimodal distribution. The first maximum, usually in autumn, is typical of "stiffer"peat associated with steeper topographic gradients, peatland margins, and degraded peatland and more often associated with "shrub"-dominated vegetation communities. The second maximum, usually in winter, is typically associated with "softer"peat typically found in low topographic gradients often featuring pool systems and Sphagnum-dominated vegetation communities. Specific conditions of "soft"and "stiff"peats are also determined by the amplitude of swelling and multi-annual average motion. Peatland restoration currently follows a re-wetting strategy; however, our approach highlights that landscape setting appears to determine the optimal endpoint for restoration. Aligning the expectation for restoration outcomes with landscape setting might optimise peatland stability and carbon storage. Importantly, deployment of this approach, based on surface motion dynamics, could support peatland mapping and management on a global scale
Characterization of an Aggregated Three-Dimensional Cell Culture Model by Multimodal Mass Spectrometry Imaging
Mass spectrometry imaging (MSI) is an established analytical tool capable of defining and understanding complex tissues by determining the spatial distribution of biological molecules. Three-dimensional (3D) cell culture models mimic the pathophysiological environment of in vivo tumors and are rapidly emerging as a valuable research tool. Here, multimodal MSI techniques were employed to characterize a novel aggregated 3D lung adenocarcinoma model, developed by the group to mimic the in vivo tissue. Regions of tumor heterogeneity and the hypoxic microenvironment were observed based on the spatial distribution of a variety of endogenous molecules. Desorption electrospray ionization (DESI)-MSI defined regions of a hypoxic core and a proliferative outer layer from metabolite distribution. Targeted metabolites (e.g., lactate, glutamine, and citrate) were mapped to pathways of glycolysis and the TCA cycle demonstrating tumor metabolic behavior. The first application of imaging mass cytometry (IMC) with 3D cell culture enabled single-cell phenotyping at 1 ÎŒm spatial resolution. Protein markers of proliferation (Ki-67) and hypoxia (glucose transporter 1) defined metabolic signaling in the aggregoid model, which complemented the metabolite data. Laser ablation inductively coupled plasma (LA-ICP)-MSI analysis localized endogenous elements including magnesium and copper, further differentiating the hypoxia gradient and validating the protein expression. Obtaining a large amount of molecular information on a complementary nature enabled an in-depth understanding of the biological processes within the novel tumor model. Combining powerful imaging techniques to characterize the aggregated 3D culture highlighted a future methodology with potential applications in cancer research and drug development
Multiscale Variability and the Comparison of Ground and Satellite Radar Based Measures of Peatland Surface Motion for Peatland Monitoring
Peatland surface motion is highly diagnostic of peatland condition. Interferometric Synthetic Aperture Radar (InSAR) can measure this at the landscape scale but requires ground validation. This necessitates upscaling from point to areal measures (80 Ă 90 m) but is hampered by a lack of data regarding the spatial variability of peat surface motion characteristics. Using a nested precise leveling approach within two areas of upland and low-lying blanket peatland within the Flow Country, Scotland, we examine the multiscale variability of peat surface motion. We then compare this with InSAR timeseries data. We find that peat surface motion varies at multiple scales within blanket peatland with decreasing dynamism with height above the water table e.g., hummocks < lawn < hollows. This trend is dependent upon a number of factors including ecohydrology, pool size/density, peat density, and slope. At the site scale motion can be grouped into central, marginal, and upland peatlands with each showing characteristic amplitude, peak timing, and response to climate events. Ground measurements which incorporate local variability show good comparability with satellite radar derived timeseries. However, current limitations of phase unwrapping in interferometry means that during an extreme drought/event InSAR readings can only qualitatively replicate peat movement in the most dynamic parts of the peatland e.g., pool systems, quaking bog
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Potential of APSIS-InSAR for measuring surface oscillations of tropical peatlands
Tropical peatland across Southeast Asia is drained extensively for production of pulpwood, palm oil and other food crops. Associated increases in peat decomposition have led to widespread subsidence, deterioration of peat condition and CO2 emissions. However, quantification of subsidence and peat condition from these processes is challenging due to the scale and inaccessibility of dense tropical peat swamp forests. The development of satellite interferometric synthetic aperture radar (InSAR) has the potential to solve this problem. The Advanced Pixel System using Intermittent Baseline Subset (APSIS, formerly ISBAS) modelling technique provides improved coverage across almost all land surfaces irrespective of ground cover, enabling derivation of a time series of tropical peatland surface oscillations across whole catchments. This study aimed to establish the extent to which APSIS-InSAR can monitor seasonal patterns of tropical peat surface oscillations at North Selangor Peat Swamp Forest, Peninsular Malaysia. Results showed that C-band SAR could penetrate the forest canopy over tropical peat swamp forests intermittently and was applicable to a range of land covers. Therefore the APSIS technique has the potential for monitoring peat surface oscillations under tropical forest canopy using regularly acquired C-band Sentinel-1 InSAR data, enabling continuous monitoring of tropical peatland surface motion at a spatial resolution of 20 m
Expression of BNIP3 in invasive breast cancer: correlations with the hypoxic response and clinicopathological features
<p>Abstract</p> <p>Background</p> <p>Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) is a pro-apoptotic member of the Bcl-2 family induced under hypoxia. Low or absent expression has recently been described in human tumors, including gastrointestinal tumors, resulting in poor prognosis. Little is known about BNIP3 expression in invasive breast cancer. The aim of the present study was to investigate the expression of BNIP3 in invasive breast cancer at the mRNA and protein level in correlation with the hypoxic response and clinicopathological features.</p> <p>Methods</p> <p>In 40 cases of invasive breast cancer, BNIP3 mRNA <it>in situ </it>hybridization was performed on frozen sections with a digoxigenin labeled anti-BNIP3 probe. Paraffin embedded sections of the same specimens were used to determine protein expression of BNIP3, Hypoxia Inducible Factor 1 alpha (HIF-1α) and its downstream targets Glucose Transporter 1 (Glut-1) and Carbonic Anhydrase (CAIX) by immunohistochemistry.</p> <p>Results</p> <p>BNIP3 mRNA was expressed in 16/40 (40%) of the cases and correlated with BNIP3 protein expression (p = 0.0218). Neither BNIP3 protein nor mRNA expression correlated with expression of HIF-1α expression or its downstream targets. Tumors which showed loss of expression of BNIP3 had significantly more often lymph node metastases (82% vs 39%, p = 0.010) and showed a higher mitotic activity index (p = 0.027). BNIP3 protein expression was often nuclear in normal breast, but cytoplasmic in tumor cells.</p> <p>Conclusion</p> <p>BNIP3 expression is lost in a significant portion of invasive breast cancers, which is correlated with poor prognostic features such as positive lymph node status and high proliferation, but not with the hypoxic response.</p
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