9 research outputs found
Combination of oviduct fluid and heparin to improve monospermic zygotes production during porcine in vitro fertilization
In vivo, the oviduct provides appropriate microenvironment conditions for monospermic fertilization and early embryo development. In addition, glycosaminoglycans such as heparin are present in the oviduct and have been shown to modulate the activity of oviduct-secreted proteins on the regulation of sperms parameters. Thus, the present study was designed to evaluate the effect of porcine oocytes exposure to oviduct fluid (OF) before in vitro fertilization (IVF; incubation of oocytes in OF for 30 minutes before IVF), during IVF (supplementation of IVF medium with 10% OF), and during IVF in combination with heparin (10% OF + 10-ÎĽg/mL heparin) on IVF parameters. Regardless of sperm concentration used (0.5, 1.5, or 4.5 Ă— 105 cells/mL), exposure of oocytes to OF led to an increased (P 0.05) of the penetration rate in comparison with the control group. This resulted in a general increase (P 0.05) on the monospermy rate in comparison with 10% OF alone. This resulted in a general reduction (P < 0.05) in the final output of the IVF system (%), which was 33 ± 6% and 52 ± 8%, for 10% OF + heparin and 10% OF, respectively. In conclusion, the OF, used in porcine IVF, exerted a beneficial effect on oocytes by reducing the incidence of polyspermy without decreasing the penetration rate. However, the association of the OF with heparin reduced the efficiency of monospermic zygotes' production.Fil: Tavares Pereira Batista, Ribrio Ivan. Centre National de la Recherche Scientifique; Francia. Universidade Estadual Do Ceara; BrasilFil: Moro, LucĂa Natalia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Centre National de la Recherche Scientifique; FranciaFil: Corbin, Emilie. Centre National de la Recherche Scientifique; FranciaFil: Alminana, Carmen. Centre National de la Recherche Scientifique; FranciaFil: Gonçalves Souza Fabjan, Joanna Maria. Universidade Estadual Do Ceara; BrasilFil: de FigueirĂŞdo Freitas, Vicente JosĂ©. Universidade Estadual Do Ceara; BrasilFil: Mermillod, Pascal. Centre National de la Recherche Scientifique; Franci
Suplementação calórica em ovelhas da raça Santa Inês submetidas à indução do estro sincronizado
The aim of this study was to evaluate the effect of a 20% increase in dietary energy during short-term estrus induction treatment on the reproductive parameters of Santa InĂŞs ewes. Females (n=43) were allocated into two experimental groups according to the amount of energy inclusion in the diet: maintenance diet or maintenance diet plus 20% energy. Ultrasound examinations were performed in order to detect ovulation. To assess sexual behavior, ewes were teased and further mated. Blood samples were collected for the determination of glucose and insulin concentrations. There was no difference (P>0.05) between groups in the following categories: ovulation rate (80.00% vs. 60.00%), largest follicle diameter (6.00 ± 0.20 vs. 5.90 ± 0.60), interval from device removal to ovulation (52.80 ± 14.87 vs. 59.01 ± 8.34 hours), animals in estrus (75.00% vs. 65.21%), interval from device removal to estrus (30.00 ± 15.49 vs. 30.00 ± 13.35 hours) and conception rate (50.00% vs. 21.73%). There were differences (P0,05) entre os grupos para: taxa de animais ovulando (80% vs 60%), diâmetro do maior folĂculo (6,00 ± 0,20 vs 5,90 ± 0,60), intervalo da retirada do implante Ă ovulação (52,80 ± 14,87 vs 59,01 ± 8,34 horas), animais em estro (75,00% vs 65,21%), intervalo da retirada do implante ao estro (30,00 ± 15,49 vs 30,00 ± 13,35 horas) e taxa de concepção (50,00% vs 21,73%). Houve diferença (P<0,05) nas concentrações de insulina e glicose. Pode-se concluir que o aumento em 20% da energia da dieta durante um protocolo curto de sincronização do estro nĂŁo alterou os parâmetros reprodutivos
Re-used progesterone devices efficiently synchronise oestrus and ovulation after autoclaving process in Toggenburg goats during the breeding season
This study compared new and previously used (and autoclaved) progesterone devices for synchronisation of oestrus during the breeding season in Toggenburg goats. Nulliparous (n = 17) or lactating (n = 50) received new intravaginal devices containing 0.3 g progesterone (CONTROL), or similar devices previously used for either 6 (USED6) or 12 (USED12) days and subsequently autoclaved. All goats received 5 mg dinoprost at device insertion and 200 IU equine chorionic gonadotropin 5 days later and all devices were removed after 6 days. After device removal, females were mated by fertile bucks. Ovarian ultrasonography was performed every 12 h after device removal until ovulation detection. Blood samples were collected for determination of plasma progesterone concentration in different moments and intervals (from 7 days before device insertion to 3 days after its removal). There was no difference (P > 0.05) among groups CONTROL, USED6 or USED12 for: oestrus response [75% (18/24), 77% (17/22) or 71% (15/21), respectively]; duration of oestrus (30.7 +/- 3.4, 31.8 +/- 1.7 or 32.8 +/- 3.4 h), percentage of ovulating goats [67% (6/9), 78% (7/9) or 56% (5/9)], ovulation rate (1.3 +/- 0.2, 1.4 +/- 0.2 or 1.8 +/- 0.4 units of corpora lutea), average follicle diameter (6.2 +/- 0.1, 6.7 +/- 0.1 or 6.8 +/- 0.3 mm) and pregnancy rate [54% (13/24), 50% (11/22) or 48% (10/21)]. Plasma progesterone concentrations were not different (P > 0.05) for does among treatments and between nulliparous and lactating females. In conclusion, autoclaved, previously used intravaginal progesterone devices are effective in synchronising oestrus and ovulation in cyclic goats during the breeding season.Conselho Nacional de Desenvolvimento CientĂfico e TecnolĂłgico (CNPq
Achados ultrassonográficos de glândula mamária, fĂgado, vesĂcula biliar, baço e rins de caprinos transgĂŞnicos para o hG-CSF durante lactação induzida
In transgenic murine models, the study of certain organs or tissues can be performed after euthanasia of some specimens. However, this practice may not be economically feasible when applied to livestock such as transgenic goats. It is necessary to use minimally invasive methods to perform in vivo studies of organs that may be affected by disorders related to the activity of the transgene, particularly during milk production, when the recombinant protein is secreted. The aim of this study was to describe ultrasonographic findings of the liver, gallbladder, spleen, kidneys, and mammary glands in transgenic goats for evaluating the effect of human granulocyte-colony stimulating factor (hG-CSF) expression in milk during induced lactation. Six female CanindĂ© goats-three transgenic (T) and three non-transgenic (NT)-were subjected to hormone therapy to induce lactation; ultrasonographic examinations of the liver, gallbladder, spleen, kidneys, and mammary gland were performed during both the hormonal therapy and the lactation period at different intervals depending on the organ being examined. On Day 16 (Day 1 = hormonal therapy initiation), all goats were lactating and presented healthy mammary glands, characterized by echogenic parenchyma showing a granular echotexture. Transgenic and non-transgenic goats were compared on the basis of measurements and ultrasound images obtained from each organ. No differences between T and NT animals were observed in the examined area for the liver, gallbladder, spleen, and kidneys. Liver and renal echogenicity and appearance of gallbladder and portal and hepatic veins were similar in all females. Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats did not show any difference from those in non-transgenic goats.Thus, these results suggest that the presence and expression of the transgene had no effect on the ultrasonographic findings of mammary gland and abdominal organs in the transgenic goats. Moreover, the findings shows that ultrasonography is a useful screening tool for clinical examination of transgenic goats; this allows the investigation of possible disorders and avoids the unnecessary use of invasive techniques.Em camundongos transgĂŞnicos, o estudo de certos ĂłrgĂŁos ou tecidos pode ser viabilizado por meio da eutanásia de alguns exemplares. Entretanto, esta prática pode nĂŁo ser economicamente viável quando aplicada a animais de produção, como caprinos transgĂŞnicos. É necessário utilizar mĂ©todos minimamente invasivos para estudar in vivo os ĂłrgĂŁos que podem estar envolvidos em desordens relacionadas Ă atividade do transgene, principalmente durante a produção de leite, quando a proteĂna recombinante Ă© secretada. O objetivo deste estudo foi descrever as caracterĂsticas ultrassonográficas de fĂgado, vesĂcula biliar, baço, rins e glândula mamária de caprinos transgĂŞnicos expressando o fator estimulante de colĂ´nias de granulĂłcitos humano (hG-CSF) no leite, durante lactação induzida. Seis fĂŞmeas caprinas da raça CanindĂ©: trĂŞs nĂŁo transgĂŞnicas (NT) e trĂŞs transgĂŞnicas (T) foram submetidas a tratamento hormonal para indução da lactação; os exames ultrassonográficos de fĂgado, vesĂcula biliar, baço, rins e glândula mamária foram realizados durante tratamento hormonal e lactação, em diferentes intervalos de acordo com o ĂłrgĂŁo. No dia 16 (Dia 1 = inĂcio do tratamento hormonal) todas as cabras estavam em lactação e apresentando uma glândula mamária saudável, caracterizada por parĂŞnquima ecogĂŞnico, com uma ecotextura granular. As cabras T e NT foram comparadas em relação Ă s mensurações e aparĂŞncia ultrassonográfica obtida de cada ĂłrgĂŁo. NĂŁo foram observadas diferenças com relação à área para exame de fĂgado, vesĂcula biliar, baço e rins entre T e NT. A ecogenicidade de fĂgado e rins, a aparĂŞncia da vesĂcula biliar e das veias porta e hepática foram similares em todas as fĂŞmeas. As mensurações ultrassonográficas dos ĂłrgĂŁos abdominais examinados nĂŁo revelaram diferenças entre animais T e NT. Assim, esses resultados sugerem que a presença e expressĂŁo do transgene nĂŁo tiveram efeito sobre as caracterĂsticas ultrassonográficas de glândula mamária e ĂłrgĂŁos abdominais nos animais transgĂŞnicos. AlĂ©m disso, os achados mostram que a ultrassonografia Ă© uma importante ferramenta para o exame clĂnico em caprinos transgĂŞnicos e que permite a investigação de possĂveis desordens, evitando o uso desnecessário de tĂ©cnicas invasivas
Innovations in cryoconservation of animal genetic resources – Practical guide: Annexes
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