Assistência nutricional nas doenças do neurônio motor/esclerose lateral amiotrófica

Patients with amyotrophic lateral sclerosis (ALS) often present changes in nutritional status. Based on weight loss and on difficulty in nutritional management, this study aims to review the different possibilities and to present guidelines concerning nutritional treatment to such patients. Diet characteristics, types of treatment and nutritional therapy indicating administration routes and discussing the details of the disease are described herein. Nutritional therapy has been a substantial therapeutic resource for ALS development.Pacientes com esclerose lateral amiotrófica (ELA) frequentemente apresentam alteração do estado nutricional. Baseado na perda ponderal de peso e na dificuldade no manejo nutricional, o objetivo desse estudo é rever as estratégias de tratamento nutricional para a manutenção do estado nutricional desses doentes. Nesse trabalho, descrevem-se as características da dieta, as formas de tratamento e terapia nutricional com indicação das vias de administração, bem como discuti-se as particularidades da doença. A terapia nutricional tem se mostrado um recurso terapêutico primordial atuante na evolução da ELA.UNIFESP-EPM Departamento de NeurologiaUNIFESP-EPMHospital Santa Marcelina Ambulatório Médico EspecialidadesUNIFESP, EPM, Depto. de NeurologiaSciEL

Biochemical characterisation of lipase from a new strain of Bacillus sp. ITP-001

Lipases are characterised mainly by catalytic versatility and application in different industrial segments. The aim of this study was to biochemically characterise a lipase from a new strain of Bacillus sp. ITP-001. The isoelectric point and molecular mass were 3.12 and 54 kDa, respectively. The optima lipase activity was 276 U g-1 at pH 7.0 and a temperature of 80 ºC, showing greater stability at pH 5.0 and 37 ºC. Enzymatic activity was stimulated by various ions and pyridine, and inhibited by Cu+ and ethanol. The values of Km and v max were 105.26 mmol and 0.116 mmol min-1 g-1, respectively determined by the Eadie-Scatchard method

Methodological aspects of patient safety culture research: A scoping Review

Investigating the safety culture has been relevant for more than two decades as a tool to improve the quality of patient care. This is a scope review of the last 20 years, whose objective is to investigate the type and prevalence of methodological approaches to patient safety culture and climate research. A review protocol was registered. The results were reported according to reports of preferred items for systematic reviews and extension of meta-analyzes for scope reviews (PRISMA-ScR). 107 studies were included. From the results, three major themes emerged for discussion, and the studies were classified into 1) Methodological approach and indeterminate use of concepts of climate and safety culture; 2) The isolated use of self-administered questionnaires in safety culture surveys and 3) A survey of the mixed methods approach in patient safety culture research. The quantitative approach was shown to be predominant, with the use of self-administered questionnaires for studies of the climate and culture of patient safety. The studies pointed out several limitations in the exclusive use of questionnaires, emphasizing the need to include multimethod research and the use of qualitative data collection techniques as an essential complement for the diagnosis of the patient safety culture

Glucosylpolyphenols as Inhibitors of Aβ-Induced Fyn Kinase Activation and Tau Phosphorylation: Synthesis, Membrane Permeability, and Exploratory Target Assessment within the Scope of Type 2 Diabetes and Alzheimer's Disease

Despite the rapidly increasing number of patients suffering from type 2 diabetes, Alzheimer's disease, and diabetes-induced dementia, there are no disease-modifying therapies that are able to prevent or block disease progress. In this work, we investigate the potential of nature-inspired glucosylpolyphenols against relevant targets, including islet amyloid polypeptide, glucosidases, and cholinesterases. Moreover, with the premise of Fyn kinase as a paradigm-shifting target in Alzheimer's drug discovery, we explore glucosylpolyphenols as blockers of Aβ-induced Fyn kinase activation while looking into downstream effects leading to Tau hyperphosphorylation. Several compounds inhibit Aβ-induced Fyn kinase activation and decrease pTau levels at 10 μM concentration, particularly the per-O-methylated glucosylacetophloroglucinol and the 4-glucosylcatechol dibenzoate, the latter inhibiting also butyrylcholinesterase and β-glucosidase. Both compounds are nontoxic with ideal pharmacokinetic properties for further development. This work ultimately highlights the multitarget nature, fine structural tuning capacity, and valuable therapeutic significance of glucosylpolyphenols in the context of these metabolic and neurodegenerative disorders