A UTILIZAÇÃO DA TERMOGRAFIA INFRAVERMELHA NA AVALIAÇÃO DE EQUINOS: Uma Revisão Sistemática

Infrared thermography is a non-invasive, painless, non-contrast and diagnostic method that analyzes the cutaneous temperature distribution by means of thermal sensors positioned close to the examined, expressing by means of a pictorial representation the surface temperature distribution of a body. The objective of this study is to analyze, through the literature, the use of thermography in equine evaluation. This is a systematic review of the literature, carried out from December 2017 to February 2018 in diferents databases. 10796 articles were found and after application of the methodological criteria 5 were left to be integrally evaluated. It was verified in the research that the use of thermography technology through infrared radiation is an effective resource in several aspects within an evaluation in horses, such as identification of lesions, fear or pain present in horses.A termografia infravermelha é um método diagnóstico não invasivo, indolor e que não necessita de contraste. Ela analisa a distribuição da temperatura cutânea através de sensores térmicos posicionados próximo ao examinado. Seus resultados são expressos por meio de uma representação pictórica da distribuição da temperatura superficial de uma determinada região do corpo. O objetivo deste estudo foi analisar, por meio da literatura, o uso da termografia na avaliação de equinos. Trata-se de uma revisão sistemática da literatura, realizada no período de dezembro de 2017 a fevereiro de 2018 em diferentes bases de dados. Foram achados 10796 artigos e após aplicação dos critérios metodológicos restaram 5 para serem integralmente avaliados. Verificou-se na pesquisa que a utilização da tecnologia da termografia através de radiação infravermelha é um recurso eficaz em diversos aspectos dentro de uma avaliação em equinos, tais como identificação de lesões, medos ou dores presentes em equinos

Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)

ABSTRACT INTRODUCTION: Trypanosoma cruzi is the etiologic agent of Chagas disease in humans, mainly in Latin America. Trypanosome stocks were isolated by hemoculture from patients followed at Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ) and studied using different approaches. METHODS: For species and genotype identification, the stocks were analyzed by parasitological techniques, polymerase chain reaction assays targeted to specific DNA sequences, isoenzyme patterns, besides sequencing of a polymorphic locus of TcSC5D gene (one stock). RESULTS: The isolates presented typical T. cruzi morphology and usually grew well in routine culture media. Metacyclic trypomastigotes were found in cultures or experimentally infected Triatoma infestans. All isolates were pure T. cruzi cultures, presenting typical 330-bp products from kinetoplast DNA minicircles, and 250 or 200-bp amplicons from the mini-exon non-transcribed spacer. Their genetic type assignment was resolved by their isoenzyme profiles. The finding of TcI in one asymptomatic patient from Paraíba was confirmed by the sequencing assay. TcVI was found in two asymptomatic individuals from Bahia and Rio Grande do Sul. TcII was identified in six patients from Pernambuco, Bahia and Minas Gerais, who presented different clinical forms: cardiac (2), digestive with megaesophagus (1), and indeterminate (3). CONCLUSIONS: The main T. cruzi genotypes found in Brazilian chronic patients were identified in this work, including TcI, which is less frequent and usually causes asymptomatic disease, unlike that in other American countries. This study emphasizes the importance of T. cruzi genotyping for possible correlations between the parasite and patient’ responses to therapeutic treatment or disease clinical manifestations

Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)

<div><p>ABSTRACT INTRODUCTION: Trypanosoma cruzi is the etiologic agent of Chagas disease in humans, mainly in Latin America. Trypanosome stocks were isolated by hemoculture from patients followed at Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ) and studied using different approaches. METHODS: For species and genotype identification, the stocks were analyzed by parasitological techniques, polymerase chain reaction assays targeted to specific DNA sequences, isoenzyme patterns, besides sequencing of a polymorphic locus of TcSC5D gene (one stock). RESULTS: The isolates presented typical T. cruzi morphology and usually grew well in routine culture media. Metacyclic trypomastigotes were found in cultures or experimentally infected Triatoma infestans. All isolates were pure T. cruzi cultures, presenting typical 330-bp products from kinetoplast DNA minicircles, and 250 or 200-bp amplicons from the mini-exon non-transcribed spacer. Their genetic type assignment was resolved by their isoenzyme profiles. The finding of TcI in one asymptomatic patient from Paraíba was confirmed by the sequencing assay. TcVI was found in two asymptomatic individuals from Bahia and Rio Grande do Sul. TcII was identified in six patients from Pernambuco, Bahia and Minas Gerais, who presented different clinical forms: cardiac (2), digestive with megaesophagus (1), and indeterminate (3). CONCLUSIONS: The main T. cruzi genotypes found in Brazilian chronic patients were identified in this work, including TcI, which is less frequent and usually causes asymptomatic disease, unlike that in other American countries. This study emphasizes the importance of T. cruzi genotyping for possible correlations between the parasite and patient’ responses to therapeutic treatment or disease clinical manifestations.</p></div