17 research outputs found
A pHluorin-based method to monitor exocytosis by release of peptidergic cargo molecules from chromaffin cells
A pHluorin-based method to monitor exocytosis by release of peptidergic cargo molecules from chromaffin cells
Controlled transportation of mesoscopic particles by enhanced spin orbit interaction of light in an optical trap
We study the effects of the spin orbit interaction (SOI) of light in an
optical trap and show that the propagation of the tightly focused trapping beam
in a stratified medium can lead to significantly enhanced SOI. For a plane
polarized incident beam the SOI manifests itself by giving rise to a strong
anisotropic linear diattenuation effect which produces polarization-dependent
off-axis high intensity side lobes near the focal plane of the trap. Single
micron-sized asymmetric particles can be trapped in the side lobes, and
transported over circular paths by a rotation of the plane of input
polarization. We demonstrate such controlled motion on single pea-pod shaped
single soft oxometalate (SOM) particles of dimension around m
over lengths up to 15 m . The observed effects are supported by
calculations of the intensity profiles based on a variation of the Debye-Wolf
approach. The enhanced SOI could thus be used as a generic means of
transporting mesoscopic asymmetric particles in an optical trap without the use
of complex optical beams or changing the alignment of the beam into the trap.Comment: 9 pages, 7 figure
Melting of the vortex lattice through intermediate hexatic fluid in a-MoGe thin film
The hexatic fluid refers to a phase in between a solid and a liquid which has
short range positional order but quasi-long range orientational order. In the
celebrated theory of Berezinskii, Kosterlitz and Thouless and subsequently
refined by Halperin, Nelson and Young, it was predicted that a 2-dimensional
hexagonal solid can melt in two steps: first, through a transformation from a
solid to a hexatic fluid which retains quasi long range orientational order and
then from a hexatic fluid to an isotropic liquid. In this paper, using a
combination of real space imaging and transport measurements we show that the
2-dimensional vortex lattice in a-MoGe thin film follows this sequence of
melting as the magnetic field is increased. Identifying the signatures of
various transitions on the bulk transport properties of the superconductor, we
construct a vortex phase diagram for a two dimensional superconductor.Comment: New Data added in this versio
Validated HPTLC Method for the Determination of Nintedanib in Bulk Drug
A simple, rapid, precise and accurate High-Performance Thin Layer Chromatography (HPTLC) method was developed and validated for the estimation of Nintedanib, a novel tyrosine kinase inhibitor used in idiopathic pulmonary fibrosis, in bulk drug. Chromatography was carried out using silica gel 60 F254 Thin Layer Chromatography (TLC) plate and mobile phase Chloroform: Methanol in the ratio 7:3 v/v. The densitometric determination was done at 386 nm. Regression analysis data for the calibration plot were indicative of a good linear relationship between response and concentration over the range of 800–3200 ng/band. The variance (r) was found to be 0.999. The Limit of Detection (LOD) and Limit of Quantitation (LOQ) were found to be 83.357 ng/band and 252.599 ng/band respectively. The method was validated according to the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use [ICH Q2(R1)] guideline. The method was precise and accurate with %RSD 0.5323 (intraday) and 0.6939 (interday) respectively and percentage recoveries in the range 99.65–101.43%
Duchenne muscular dystrophy: Case report and review
Muscular dystrophies are a clinically and heterogeneous group of disorders that all share clinical characteristics of progressive muscular weakness. Duchenne muscular dystrophy (DMD) is the most common X-linked disorder muscular dystrophy in children, presenting in early childhood and characterized by proximal muscle weakness and calf hypertrophy in affected boys. There is usually delay in motor development and eventually wheelchair confinement followed by premature death from cardiac or respiratory complications. Treatment modalities such as corticosteroid therapy and use of intermittent positive pressure ventilation have provided improvements in function, ambulation, quality of life, and life expectancy, although novel therapies still aim to provide a cure for this devastating disorder. Here, we present a case of DMD in a 12-year-old male with remarkable clinical and oral manifestations