“Becoming a Family”: Developmental Processes Represented in Blended Family Discourse

We adopted a process-focus in order to gain a deeper understanding of how (step) blended family members experiencing different developmental pathways discursively represented their processes of becoming a family. Using a qualitative/interpretive method, we analyzed 980 pages of interview transcripts with stepparents and stepchildren. We studied the first four years of family development, using the five developmental pathways developed by Baxter, Braithwaite, and Nicholson (1999). Three salient issues identified in the family experiences were boundary management, solidarity, and adaptation. While the negotiation of these issues varied across the five trajectories, there were commonalities across family experiences that helped determine whether families had a successful experience of becoming a family. Implications for blended family researchers and practitioners are also discussed

Scenario development and multi-criteria analysis for Morocco’s future electricity system in 2050. Summary of workshop results

In the scope of the MENA SELECT research project, a workshop was conducted in Rabat, Morocco, from 23 to 24 May 2016. During the workshop, stakeholders from different national societal groups discussed and developed future settings of Morocco’s power supply with the help of an advanced spreadsheet model, accompanied by an evaluation of the developed scenarios. In this paper, the results of the workshop are summarized. In the first part of the workshop, the participants were introduced to the modeling approach. Central input parameters, procedures and assumptions were presented. This formed the basis for the subsequent development of scenarios on Morocco’s power supply until 2050. With the help of the spreadsheet model, four consistent scenarios were developed, reaching renewable energy shares ranging from approximately 60 to 100 per cent. In the second part of the workshop, the workshop participants weighted the developed scenarios. For this purpose a multi-criteria analysis was conducted, which included quantitative and qualitative criteria for fossil fuels and renewable energy technologies. The participants weighted the selected criteria against each other according to the preferences of the respective institutions they represented. In combination with the criteria performance for each technology a ranking of the developed electricity scenarios took place resulting in the fact that the workshop participants would accept the scenario with the highest share of renewable energies in 2050 most. The workshop successfully illustrated that it was possible to develop options of Morocco’s future power supply by combining technical and economic parameters that were acceptable to the workshop participants

Monoterpene indole alkaloids from Vinca minor L. (Apocynaceae): Identification of new structural scaffold for treatment of Alzheimer's disease

One undescribed indole alkaloid together with twenty-two known compounds have been isolated from aerial parts of Vinca minor L. (Apocynaceae). The chemical structures of the isolated alkaloids were determined by a combination of MS, HRMS, 1D, and 2D NMR techniques, and by comparison with literature data. The NMR data of several alkaloids have been revised, corrected, and missing data have been supplemented. Alkaloids isolated in sufficient quantity were screened for their in vitro acetylcholinesterase (AChE; E.C. 3.1.1.7) and butyrylcholinesterase (BuChE; E.C. 3.1.1.8) inhibitory activity. Selected compounds were also evaluated for prolyl oligopeptidase (POP; E.C. 3.4.21.26), and glycogen synthase 3β-kinase (GSK-3β; E.C. 2.7.11.26) inhibition potential. Significant hBuChE inhibition activity has been shown by (−)-2-ethyl-3[2-(3-ethylpiperidinyl)-ethyl]-1H-indole with an IC50 value of 0.65 ± 0.16 μM. This compound was further studied by enzyme kinetics, along with in silico techniques, to reveal the mode of inhibition. This compound is also predicted to cross the blood-brain barrier (BBB) through passive diffusion

Riluzole-Rasagiline Hybrids: Toward the Development of Multi-Target-Directed Ligands for Amyotrophic Lateral Sclerosis

Polypharmacology is a new trend in amyotrophic lateral sclerosis (ALS) therapy and an effective way of addressing a multifactorial etiology involving excitotoxicity, mitochondrial dysfunction, oxidative stress, and microglial activation. Inspired by a reported clinical trial, we converted a riluzole (1)-rasagiline (2) combination into single-molecule multi-target-directed ligands. By a ligand-based approach, the highly structurally integrated hybrids 3-8 were designed and synthesized. Through a target- and phenotypic-based screening pipeline, we identified hit compound 6. It showed monoamine oxidase A (MAO-A) inhibitory activity (IC50 = 6.9 mu M) rationalized by in silico studies as well as in vitro brain permeability. By using neuronal and non-neuronal cell models, including ALS-patient-derived cells, we disclosed for 6 a neuroprotective/neuroinflammatory profile similar to that of the parent compounds and their combination. Furthermore, the unexpected MAO inhibitory activity of 1 (IC50 = 8.7 mu M) might add a piece to the puzzle of its anti-ALS molecular profile

Component traits of plant water use are modulated by vapour pressure deficit in pearl millet (Pennisetum glaucum (L.) R.Br.)

Traits influencing plant water use eventually define the fitness of genotypes for specific rainfall environments. We assessed the response of several water use traits to vapour pressure deficit (VPD) in pearl millet (Pennisetum glaucum (L.) R.Br.) genotypes known to differ in drought adaptation mechanisms: PRLT 2/89–33 (terminal drought-adapted parent), H 77/833–2 (terminal drought-sensitive parent) and four near-isogenic lines introgressed with a terminal drought tolerance quantitative trait locus (QTL) from PRLT 2/89–33 (ICMR01029, ICMR01031, ICMR02042, and ICMR02044). Plant water use traits at various levels of plant organisation were evaluated in seven experiments in plants exposed either transiently or over the long term to different VPD regimes: biomass components, transpiration (water usage per time unit) and transpiration rate (TR) upon transient VPD increase (g H2O cm–2 h–1)), transpiration efficiency (g dry biomass per kg H2O transpired), leaf expansion rate (cm per thermal time unit) and root anatomy (endodermis dimensions)). High VPD decreased biomass accumulation by reducing tillering, the leaf expansion rate and the duration of leaf expansion; decreased root endodermis cell size; and increased TR and the rate of TR increase upon gradual short-term VPD increases. Such changes may allow plants to increase their water transport capacity in a high VPD environment and are genotype-specific. Some variation in water use components was associated with terminal drought adaptation QTL. Knowledge of water use traits’ plasticity in growth environments that varied in evaporative demand, and on their genetic determinacy, is necessary to develop trait-based breeding approaches to complex constraints

Molecular modelling of the GIR1 branching ribozyme gives new insight into evolution of structurally related ribozymes

Twin-ribozyme introns contain a branching ribozyme (GIR1) followed by a homing endonuclease (HE) encoding sequence embedded in a peripheral domain of a group I splicing ribozyme (GIR2). GIR1 catalyses the formation of a lariat with 3 nt in the loop, which caps the HE mRNA. GIR1 is structurally related to group I ribozymes raising the question about how two closely related ribozymes can carry out very different reactions. Modelling of GIR1 based on new biochemical and mutational data shows an extended substrate domain containing a GoU pair distinct from the nucleophilic residue that dock onto a catalytic core showing a different topology from that of group I ribozymes. The differences include a core J8/7 region that has been reduced and is complemented by residues from the pre-lariat fold. These findings provide the basis for an evolutionary mechanism that accounts for the change from group I splicing ribozyme to the branching GIR1 architecture. Such an evolutionary mechanism can be applied to other large RNAs such as the ribonuclease P

On the Dynamic Stability of Cool Supergiant Atmospheres

We have developed a new formalism to compute the thermodynamic coefficient Gamma1 in the theory of stellar and atmospheric stability. We generalize the classical derivation of the first adiabatic index, which is based on the assumption of thermal ionization and equilibrium between gas and radiation temperature, towards an expression which incorporates photo-ionization due to radiation with a temperature T_rad different from the local kinetic gas temperature.Our formalism considers the important non-LTE conditions in the extended atmospheres of supergiant stars. An application to the Kurucz grid of cool supergiant atmospheres demonstrates that models with T_rad =~ T_eff between 6500 K and 7500 K become most unstable against dynamic perturbations, according to Ledoux' stability integral . This results from Gamma1 and acquiring very low values, below 4/3, throughout the entire stellar atmosphere, which causes very high gas compression ratios around these effective temperatures. Based on detailed NLTE-calculations, we discuss atmospheric instability of pulsating massive yellow supergiants, like the hypergiant rho Cas (Ia+), which exist in the extension of the Cepheid instability strip, near the Eddington luminosity limit.Comment: 54 pages including figures and the Appendix, 7 figures, Accepted for The Astrophysical Journal, Main Journal, 558, Sept. 200