Apparent lipid and fatty acid digestion, retention of lipid and growth performance in Caspian salmon (Salmo trutta caspius) fry fed dietary n-3 highly unsaturated fatty acids and vitamin E

A 10-week feeding experiment was conducted to investigate the effect of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) and vitamin E interaction on growth, apparent digestibility, apparent retention and utilization of lipid in Caspian salmon fry. Six experimental diets combining three different dietary levels of n-3 HUFAs (L: low: 1+0.5, DHA+EPA, M: medium 2+1, DHA+EPA, H: high 4+2 DHA +EPA g/100g diet) with two different levels of vitamin E (L: low 300 and H: high 1000 mg/kg diet): LL, LH, ML, MH, HL and HH (HUFA/vitamin E) were investigated. Fry with initial mean (±SD) body weight of 600 ± 25 (mg) were randomly distributed in tanks and fed to apparent satiation. Increase in dietary HUFA and vitamin E markedly improved larval growth. The results showed that the survival of Caspian salmon fry was not affected by dietary omega-3 HUFA and vitamin E. C20:5n-3 and C22:6n-3 fatty acids content of polar and neutral lipids positively correlated with their concentration on diets. Apparent digestibility and apparent retention of total lipid was significantly affected by dietary HUFA. The results showed that n-3 highly unsaturated fatty acids and vitamin E had positive effects on growth performance, lipid metabolism and apparent digestibility of Caspian salmon fry

Prognostic factors for esophageal squamous cell Carcinoma-A Population-Based study in Golestan province, Iran, a high incidence area

Golestan Province in northern Iran is an area with a high incidence of esophageal squamous cell carcinoma (ESCC). We aimed to investigate prognostic factors for ESCC and survival of cases in Golestan, on which little data were available. We followed-up 426 ESCC cases participating in a population-based case-control study. Data were analyzed using the Kaplan-Meier method and the Cox proportional hazard models. Median survival was 7 months. Age at diagnosis was inversely associated with survival, but the association was disappeared with adjustment for treatment. Residing in urban areas (hazard ratio, HR = 0.70; 95 CI 0.54-0.90) and being of non-Turkmen ethnic groups (HR = 0.76; 95 CI 0.61-0.96) were associated with better prognosis. In contrast to other types of tobacco use, nass (a smokeless tobacco product) chewing was associated with a slightly poorer prognosis even in models adjusted for other factors including stage of disease and treatment (HR = 1.38; 95 CI 0.99-1.92). Opium use was associated with poorer prognosis in crude analyses but not in adjusted models. Almost all of potentially curative treatments were associated with longer survival. Prognosis of ESCC in Golestan is very poor. Easier access to treatment facilities may improve the prognosis of ESCC in Golestan. The observed association between nass chewing and poorer prognosis needs further investigations; this association may suggest a possible role for ingestion of nass constituents in prognosis of ESCC. © 2011 Aghcheli et al

The effect of isolated Lactobacillus from gut of Barbus grypus on growth performance, survival and gut microflora of common carp (Cyprinus carpio)

This study evaluated the impact of dietary supplementation bacteria, Lactobacillus plantarum and Lactobacillus bulgaricus isolated from the intestine of Barbus grypus on growth performance, survival and intestinal flora of common carp. For this purpose 480 common carp (average initial weight of 40 ± 6g) were randomly divided to 4 treatments (with three replications) and fed with diet containing 5×107 CFU/g L.plantarum (Group 1), L.bulgaricus (Group 2), Lactobacillus casei (Group3) and a diet without probiotic supplementation (As control group) for 60 days. To evaluate the persistent of the bacteria in digestive system, from the 60 th day to75 th day, experimental fish were fed with diet without probiotics (control). Results showed that Specific growth rate of fish fed diets containing Lactobacillus significantly higher compared with control group (p<0.05). Fish fed diets containing L.casei had significantly lower FCR than the control group on 60 th and 75 th of trial (p<0.05). The protein efficiency ratio and specific growth rate of fish fed the isolated bacteria at different times of trial was higher compared to fish fed than control diet. In general, results of intestinal micro flora assessment showed that Lactobacillus bacteria significantly increased in fish fed with dietary probiotic at 30 and 60 of trial (p<0.05) and groups 2 and 3 showed the greatest amount of Lactobacillus compared to the control group after 30 days of feeding. Theresults of this study showed that the addition of lactobacillus bacteria isolated from the intestines of Barbus grypus in the diet of common carp increased the beneficial micro flora population of intestinal microflora and ultimately improved growth performance and feed utilization

An Intrinsic Description of the Nonlinear Aeroelasticity of Very Flexible Wings

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90662/1/AIAA-2011-1917-972.pd

Evaluation of anthropometric indices, eating habits, and their relationship with CD4 level in individuals with HIV/AIDS

Introduction: Malnutrition is one of the important and complex complications of human immunodeficiency virus (HIV), and anthropometry is a valuable clinical indicator in health planning and policy making among HIV/acquired immunodeficiency syndrome (AIDS) patients. The purpose of this study was to evaluate anthropometric indices, eating habits, and their association with CD4 in individuals with HIV/AIDS. Material and methods: This descriptive-analytical study was conducted on all individuals with HIV/ AIDS over 20 years of age, who referred to a behavioral diseases counseling center in Iran in 2018. Census sampling method was conducted, and 122 patients were selected. Anthropometric indices were measured using standard methods, and nutritional status questionnaire was used to assess patients’ nutritional status. Results: The mean body mass index in women and men were 25 and 22 kg/m2, respectively. Abdominal obesity was 0.89 and 0.87 cm in female and male patients, respectively. Furthermore, 64% of patients had poor eating habits. Among food groups, consumption of water (p = 0.05) and carbonated drinks (p = 0.034) were significantly different between men and women. Among dietary groups, only meat and legumes group had a significant association with CD4 level (p = 0.047). Conclusions: Although 57% of patients with HIV infection were within normal range, abdominal obesity was higher than standard values, and two thirds of the participants presented poor eating habits. Therefore, nutritional interventions are of great importance in this group of patients

Candidate gene association study of esophageal squamous cell carcinoma in a high-risk region in Iran

There is a region with a high risk for esophageal squamous cell carcinoma (ESCC) in the northeast of Iran. Previous studies suggest that hereditary factors play a role in the high incidence of cancer in the region. We selected 22 functional variants (and 130 related tagSNPs) from 15 genes that have been associated previously with the risk of ESCC. We genotyped a primary set of samples from 451 Turkmens (197 cases and 254 controls). Seven of 152 variants were associated with ESCC at the P = 0.05 level; these single nucleotide polymorphisms were then studied in a validation set of 549 cases and 1,119 controls, which included both Turkmens and non-Turkmens. The association observed for a functional variant in ADH1B was confirmed in the validation set, and that of a tagSNP in MGMT, the association was borderline significant in the validation set, after correcting for multiple testing. The other 5 variants that were associated in the primary set were not significantly associated in the validation set. The histidine allele at codon 48 of ADH1B gene was associated with a significantly decreased risk of ESCC in the joint data set (primary and validation set) under a recessive model (odds ratio, 0.41; 95 confidence interval, 0.29-0.76; P = 4 × 10⁻⁴). The A allele of the rs7087131 variant of MGMT gene was associated with a decreased risk of ESCC under a dominant model (odds ratio, 0.79; 95 confidence interval, 0.64-0.96; P = 0.02). These results support the hypothesis that genetic predisposition plays a role in the high incidence of ESSC in Iran. ©2009 American Association for Cancer Research

Evaluating the Anti-Leech Effects of Methanolic Extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica. World&apos;s Vet

ABSTRACT Leeches had several complications such as pain, itching, inflammation, severe anemia, short-term bleeding, hypersensitivity, and anaphylactic reactions in their hosts. Harmal Peganum harmala L. is used as an analgesic and anti-inflammatory agent and it has antibacterial activity. Olive Olea europaea L. has antibacterial, anti-viral, hypoglycemic and the relaxation of blood vessels properties. Antioxidant properties of olive also had been reported. This study was carried out to detect the effects of methanolic extracts of P. harmala L. and O. europaea L. on L. nilotica immature form. In 2011, 55 immature leeches collected from the southern area of Ilam province were prepared. The methanolic extract of O. europaea L and P. harmala L. were compared with levamisole as the control drug. Distilled water was evaluated as the placebo group which investigated L. nilotica using anti-leech assay. Then extract and drugs were added and their effects were screened for 720 min and time to paralyze, kill and death of each leech was recorded. The results showed that olive methanolic extractions (600 and 300mg) could kill the leeches in an average time of 145±77.57 and171±33.28 min, respectively. An average death time for levamisole was found to be 15±7.49 min. The highest effectiveness was found for levamisole at dose 300 mg. Methanol extracts of the Harmal (300 and 600 μg/m) and springs water showed no anti-leech. In sum, olive plant could use for anti Limnatis nilotica expenditure

Gastric atrophy and oesophageal squamous cell carcinoma: Possible interaction with dental health and oral hygiene habit

Background:Gastric fundal atrophy has been hypothesised to increase the risk of oesophageal squamous cell carcinoma (OSCC), but studies have shown inconsistent results.Methods:We measured serum pepsinogen I (PGI) and pepsinogen II (PGII) among 293 incident cases and 524 matched neighbourhood controls in a high-risk area of Northern Iran. Conditional logistic regression model was used to estimate odds ratios (ORs) and their 95% confidence intervals (CIs).Results:After controlling for age, sex, residence area and other potential confounders, gastric atrophy (defined by a validated criterion, PGI <55 μg dl-1) was associated with a two-fold increased risk (OR=2.01, 95% CI: 1.18, 3.45) of OSCC in the absence of nonatrophic pangastritis (defined as PGII <11.8 μg dl-1). Stratification by PGII decreased the misclassification errors due to cancer-induced gastritis. Presence of both poor dental health, indicated by higher than median sum of decayed, missing, and filled teeth (DMFT score), and gastric atrophy further increased the risk of OSCC (OR=4.15, 95% CI: 2.04, 8.42) with relative excess risk due to interaction (RERI) of 1.47 (95% CI: 1.15, 4.1). Coexistence of poor oral hygiene habit with gastric atrophy elevated OSCC risk eight times (OR=8.65, 95% CI: 3.65, 20.46) and the additive interaction index was marginally statistically significant (RERI=4.34, 95% CI: 1.07, 9.76).Conclusion:Gastric atrophy is a risk factor for OSCC, and poor dental health and oral hygiene habit may act synergistically in increasing the risk. © 2012 Cancer Research UK

Golestan cohort study of oesophageal cancer: feasibility and first results

To investigate the incidence of oesophageal cancer (EC) in the Golestan province of North-East Iran, we invited 1349 rural and urban inhabitants of Golestan province aged 35–80 to undergo extensive lifestyle interviews and to provide biological samples. The interview was repeated on a subset of 130 participants to assess reliability of questionnaire and medical information. Temperature at which tea was consumed was measured on two occasions by 110 subjects. Samples of rice, wheat and sorghum were tested for fumonisin contamination. An active follow-up was carried out after 6 and 12 months. A total of 1057 subjects (610 women and 447 men) participated in this feasibility study (78.4% participation rate). Cigarette smoking, opium and alcohol use were reported by 163 (13.8%), 93 (8.8%) and 39 (3.7%) subjects, respectively. Tobacco smoking was correlated with urinary cotinine (κ=0.74). Most questionnaire data had κ >0.7 in repeat measurements; tea temperature measurement was reliable (κ=0.71). No fumonisins were detected in the samples analysed. During the follow-up six subjects were lost (0.6%), two subjects developed EC (one dead, one alive); in all, 13 subjects died (with cause of death known for 11, 84.6%). Conducting a cohort study in Golestan is feasible with reliable information obtained for suspected risk factors; participants can be followed up for EC incidence and mortality

p16INK4a hypermethylation and p53, p16 and MDM2 protein expression in Esophageal Squamous Cell Carcinoma

<p>Abstract</p> <p>Background</p> <p>Tumor suppressor genes <it>p53 </it>and <it>p16</it>^INK4aand the proto-oncogene <it>MDM2 </it>are considered to be essential G1 cell cycle regulatory genes whose loss of function is associated with ESCC carcinogenesis. We assessed the aberrant methylation of the <it>p16 </it>gene and its impact on <it>p16</it>^{<it>INK4a </it>}protein expression and correlations with <it>p53 </it>and <it>MDM2 </it>protein expressions in patients with ESCC in the Golestan province of northeastern Iran in which ESCC has the highest incidence of cancer, well above the world average.</p> <p>Methods</p> <p>Cancerous tissues and the adjacent normal tissue obtained from 50 ESCC patients were assessed with Methylation-Specific-PCR to examine the methylation status of <it>p16</it>. The expression of <it>p16</it>, <it>p53 </it>and <it>MDM2 </it>proteins was detected by immunohistochemical staining.</p> <p>Results</p> <p>Abnormal expression of <it>p16 </it>and <it>p53</it>, but not <it>MDM2</it>, was significantly higher in the tumoral tissue. <it>p53 </it>was concomitantly accumulated in ESCC tumor along with <it>MDM2 </it>overexpression and <it>p16 </it>negative expression. Aberrant methylation of the <it>p16</it>^{<it>INK4a </it>}gene was detected in 31/50 (62%) of esophageal tumor samples, while two of the adjacent normal mucosa were methylated (P < 0.001). <it>p16</it>^{<it>INK4a </it>}aberrant methylation was significantly associated with decreased <it>p16 </it>protein expression (P = 0.033), as well as the overexpression of <it>p53 </it>(P = 0.020).</p> <p>Conclusions</p> <p><it>p16 </it>hypermethylation is the principal mechanism of <it>p16 </it>protein underexpression and plays an important role in ESCC development. It is associated with p53 protein overexpression and may influence the accumulation of abnormally expressed proteins in <it>p53-MDM2 </it>and <it>p16-Rb </it>pathways, suggesting a possible cross-talk of the involved pathways in ESCC development.</p