A Generalized Low Order Model for Vortex Shedding From a Tandem Cylinder Arrangement Using Delay Coupled Van der Pol Oscillators

A generalized low order model (LOM) for the fluctuating lift coefficient caused by vortex shedding from a tandem cylinder pair is proposed to expand upon models from previous authors. This model could provide a reduced computational time method for collecting qualitative and quantitive data from a tandem shedding pair. A delay coupled system with sufficient bifurcation characteristics is developed to account for the different flow regimes (extended-body, reattachment, and co-shedding) which occur as cylinder spacing is varied. Coefficient and parameter fitting is performed to fit experimental data. Finally, results and physical interpretations of the interactions in the model are discussed. It was found that many aspects of the flow at varying L/D ratios could be modeled by the LOM, including vortex suppression in the forward cylinder at the critical spacing, and amplitude growth in the rear cylinder in the co-shedding regime

Design and Performance of Odyssey IV: A Deep Ocean Hover-Capable AUV

The Odyssey IV class AUV was designed to fill the evolving needs of research and industry for a deep rated (6000 meter) vehicle, which is capable of both efficient cruising and precise hovering. This AUV is powerful enough to reject currents typical in the open ocean environment and yet small enough to be deployed from a small fishing boat. The thruster layout, two vectored side thrusters and two fixed cross-body thrusters, allow for 4-DOF control, which gives this vehicle precision and flexibility not possible in previous Odyssey class AUVs. An adaptable payload area allows the mounting of sensors, actuators, or other hardware suitable to a particular mission. The dynamic control layer of our behavior-based MOOS software was completely redesigned to take advantage of the capabilities of this vehicle. This is also the first platform to utilize new graphical controls and database-driven logging which increase operator efficiency and make the vehicle safer to operate. Odyssey IV's intended uses include survey and inspection of cold water corals, fisheries, archaeological sites, and subsea infrastructure. It will also serve as a research platform for computer vision-based servoing and acoustic supervisory control. This paper will document the design considerations and implementation of the Odyssey IV, as well as report on a series of field tests culminating in its first scientific deployment at Georges Bank, observing and mapping the invasive tunicate Didemnum.United States. National Oceanic and Atmospheric Administration (Grant NA16RG2255); United States. National Oceanic and Atmospheric Administration (Grant NA06AOR4170019

Remote identification of the invasive tunicate Didemnum vexillum using reflectance spectroscopy

Benthic coverage of the invasive tunicate Didemnum vexillum on Georges Bank is largely unknown. Monitoring of D. vexillum coverage is vital to understanding the impact this invasive species will have on the productive fishing grounds of Georges Bank. Here we investigate using reflectance spectroscopy as a method for remote identification of D. vexillum. Using two different systems, a NightSea Dive-Spec and a combination of LED light sources with a hyperspectral radiometer, we collected in-situ measurements of reflectance from D. vexillum colonies. In comparison to reflectance spectra of other common benthic substrates, D. vexillum appears to have a unique spectral signature between 500 and 600 nm. Measuring the slope of the spectrum between these wavelengths appears to be the most robust method for spectral identification. Using derivative analysis or principal component analysis, the reflectance spectra of D. vexillum can be identified among numerous other spectra of common benthic substrates. An optical system consisting of a radiometer, light source, and camera was deployed on a remotely operated vehicle to test the feasibility of using reflectance to assess D. vexillum coverage. Preliminary results, analyzed here, prove the method to be successful for the areas we surveyed and open the way for its use on large-scale surveys

An Assessment of Dialysis Provider's Attitudes towards Timing of Dialysis Initiation in Canada

Background: Physicians' perceptions and opinions may influence when to initiate dialysis. Objective: To examine providers' perspectives and opinions regarding the timing of dialysis initiation. Design: Online survey. Setting: Community and academic dialysis practices in Canada. Participants: A nationally-representative sample of dialysis providers. Measurements and Methods: Dialysis providers opinions assessing reasons to initiate dialysis at low or high eGFR. Responses were obtained using a 9-point Likert scale. Early dialysis was defined as initiation of dialysis in an individual with an eGFR greater than or equal to 10.5 ml/min/m 2 . A detailed survey was emailed to all members of the Canadian Society of Nephrology (CSN) in February 2013. The survey was designed and pre-tested to evaluate duration and ease of administration. Results: One hundred and forty one (25% response rate) physicians participated in the survey. The majority were from urban, academic centres and practiced in regionally administered renal programs. Very few respondents had a formal policy regarding the timing of dialysis initiation or formally reviewed new dialysis starts (N = 4, 3.1%). The majority of respondents were either neutral or disagreed that late compared to early dialysis initiation improved outcomes (85–88%), had a negative impact on quality of life (89%), worsened AVF or PD use (84–90%), led to sicker patients (83%) or was cost effective (61%). Fifty-seven percent of respondents felt uremic symptoms occurred earlier in patients with advancing age or co-morbid illness. Half (51.8%) of the respondents felt there was an absolute eGFR at which they would initiate dialysis in an asymptomatic patient. The majority of respondents would initiate dialysis for classic indications for dialysis, such as volume overload (90.1%) and cachexia (83.7%) however a significant number chose other factors that may lead them to early dialysis initiation including avoiding an emergency (28.4%), patient preference (21.3%) and non-compliance (8.5%). Limitations: 25% response rate. Conclusions: Although the majority of nephrologists in Canada who responded followed evidence-based practice regarding the timing of dialysis initiation, knowledge gaps and areas of clinical uncertainty exist. The implementation and evaluation of formal policies and knowledge translation activities may limit potentially unnecessary early dialysis initiation

Ginger Compound [6]-Shogaol and Its Cysteine-Conjugated Metabolite (M2) Activate Nrf2 in Colon Epithelial Cells in Vitro and in Vivo

In this study, we identified Nrf2 as a molecular target of [6]-shogaol (6S), a bioactive compound isolated from ginger, in colon epithelial cells in vitro and in vivo. Following 6S treatment of HCT-116 cells, the intracellular GSH/GSSG ratio was initially diminished but was then elevated above the basal level. Intracellular reactive oxygen species (ROS) correlated inversely with the GSH/GSSG ratio. Further analysis using gene microarray showed that 6S upregulated the expression of Nrf2 target genes (AKR1B10, FTL, GGTLA4, and HMOX1) in HCT-116 cells. Western blotting confirmed upregulation, phosphorylation, and nuclear translocation of Nrf2 protein followed by Keap1 decrease and upregulation of Nrf2 target genes (AKR1B10, FTL, GGTLA4, HMOX1, and MT1) and glutathione synthesis genes (GCLC and GCLM). Pretreatment of cells with a specific inhibitor of p38 (SB202190), PI3K (LY294002), or MEK1 (PD098059) attenuated these effects of 6S. Using ultra-high-performance liquid chromatography–tandem mass spectrometry, we found that 6S modified multiple cysteine residues of Keap1 protein. In vivo 6S treatment induced Nrf2 nuclear translocation and significantly upregulated the expression of MT1, HMOX1, and GCLC in the colon of wild-type mice but not Nrf2–/– mice. Similar to 6S, a cysteine-conjugated metabolite of 6S (M2), which was previously found to be a carrier of 6S in vitro and in vivo, also activated Nrf2. Our data demonstrated that 6S and its cysteine-conjugated metabolite M2 activate Nrf2 in colon epithelial cells in vitro and in vivo through Keap1-dependent and -independent mechanisms

Intra–Party Democracy and Responsiveness to Rival Parties’ Policies

Objective: We address whether intraparty democracy conditions political parties' responsiveness to rival parties' policy shifts. Method: We estimate parameters of a spatial econometric model of parties' policy positions in eleven established democracies. Results: Internally democratic parties respond to shifts in rival parties' policies, and internally undemocratic parties do no t respond rival parties' policy shifts. Conclusion: We argue that this occurs because intra-party deliberation provides a channel through which rival parties influence their competitors' policies. Because rank-and-file party members are influenced by deliberative processes more than party leaders, and the policy goals of internally democratic parties' policies are heavily influenced by their party members deliberative processes lead democratic parties to respond to shifts in rival parties' policy positions. This work has important implication s for our understanding of parties' election strategies, intra-party politics, and how policies diffuse between parties competing in the same election