Biphasic Calcium Phosphate Bioceramics for Orthopaedic Reconstructions: Clinical Outcomes

BCP are considered the most promising biomaterials for bone reconstruction. This study aims at analyzing the outcomes of patients who received BCP as bone substitutes in orthopaedic surgeries. Sixty-six patients were categorized according to the etiology and morphology of the bone defects and received scores after clinical and radiographic evaluations. The final results corresponded to the combination of both parameters and varied from 5 (excellent result) to 2 or lower (poor result). Most of the patients who presented cavitary defects or bone losses due to prosthesis placement or revision, osteotomies, or arthrodesis showed good results, and some of them excellent results. However, patients with segmental defects equal or larger than 3 cm in length were classified as moderate results. This study established clinical parameters where the BCP alone can successfully support the osteogenic process and where the association with other tissue engineering strategies may be considered

Comprehensive Profiling of N‑Linked Glycosylation Sites in HeLa Cells Using Hydrazide Enrichment

The adenocarcinoma cell line HeLa serves as a model system for cancer research in general and cervical cancer in particular. In this study, hydrazide enrichment in combination with state-of-the art nanoLC−MS/MS analysis was used to profile N-linked glycosites in HeLa cells. N-Linked glycoproteins were selectively enriched in HeLa cells by the hydrazide capture method, which isolates all glycoproteins independent of their glycans. Nonglycosylated proteins were removed by extensive washing. N-Linked glycoproteins were identified with the specific NXT/S motif and deamidated asparagine (N). Deglycosylation was carried out in both H_2 (^16)O and H_2 ^(18)O to confirm the deamidation. NanoLC−MS/MS analysis indicated that the method selectively enriched at least 100 fold N-linked glycosites in HeLa cells. When both the membrane and cytosolic fractions were used, a total of 268 unique N-glycosylation sites were identified corresponding to 106 glycoproteins. Bioinformatic analysis revealed that most of the glycoproteins identified are known to have an impact on cancer and have been proposed as biomarkers

California communities deal with conflict and adjustment at the urban-agricultural edge

About 2.5 million agricultural acres are located adjacent or in close proximity to nonfarm residences in California, leading to widespread farm-residential conflicts. This exploratory study compared high- and low-conflict edges in four crop-growing communities in two counties. (A separate analysis of San Diego County in a sidebar compares two edge situations involving animal and nursery operations.) We present tentative generalizations about conflict variations, sources and solutions. High conflict levels were largely due to residents’ unfamiliarity with agricultural activities, although conflict levels were also related to specific farming practices. We also pose questions to guide further and more systematic research on the edge issue in California agriculture

California communities deal with conflict and adjustment at the urban-agricultural edge

About 2.5 million agricultural acres are located adjacent or in close proximity to nonfarm residences in California, leading to widespread farm-residential conflicts. This exploratory study compared high- and low-conflict edges in four crop-growing communities in two counties. (A separate analysis of San Diego County in a sidebar compares two edge situations involving animal and nursery operations.) We present tentative generalizations about conflict variations, sources and solutions. High conflict levels were largely due to residents’ unfamiliarity with agricultural activities, although conflict levels were also related to specific farming practices. We also pose questions to guide further and more systematic research on the edge issue in California agriculture