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Effect of inorganic-to-organic mass ratio on the heterogeneous OH reaction rates of erythritol: Implications for atmospheric chemical stability of 2-methyltetrols
The 2-methyltetrols have been widely chosen as chemical tracers for isoprene-derived secondary organic aerosols. While they are often assumed to be relatively unreactive, a laboratory study reported that pure erythritol particles (an analog of 2-methyltetrols) can be heterogeneously oxidized by gas-phase OH radicals at a significant rate. This might question the efficacy of these compounds as tracers in aerosol source-apportionment studies. Additional uncertainty could arise as organic compounds and inorganic salts often coexist in atmospheric particles. To gain more insights into the chemical stability of 2-methyltetrols in atmospheric particles, this study investigates the heterogeneous OH oxidation of pure erythritol particles and particles containing erythritol and ammonium sulfate (AS) at different dry inorganic-to-organic mass ratios (IOR) in an aerosol flow tube reactor at a high relative humidity of 85 %. The same reaction products are formed upon heterogenous OH oxidation of erythritol and erythritol-AS particles, suggesting that the reaction pathways are not strongly affected by the presence and amount of AS. On the other hand, the effective OH uptake coefficient, eff, is found to decrease by about a factor of 20 from 0:450:025 to 0:020:001 when the relative abundance of AS increases and the IOR increases from 0.0 to 5.0. One likely explanation is the presence of dissolved ions slows down the reaction rates by decreasing the surface concentration of erythritol and reducing the frequency of collision between erythritol and gas-phase OH radicals at the particle surface. Hence, the heterogeneous OH reactivity of erythritol and likely 2-methyltetrols in atmospheric particles would be slower than previously thought when the salts are present. Given 2-methyltetrols often coexist with a significant amount of AS in many environments, where ambient IOR can vary from 1:89 to 250, our kinetic data would suggest that 2-methyltetrols in atmospheric particles are likely chemically stable against heterogeneous OH oxidation under humid conditions
Physics-assisted Generative Adversarial Network for X-Ray Tomography
X-ray tomography is capable of imaging the interior of objects in three
dimensions non-invasively, with applications in biomedical imaging, materials
science, electronic inspection, and other fields. The reconstruction process
can be an ill-conditioned inverse problem, requiring regularization to obtain
satisfactory reconstructions. Recently, deep learning has been adopted for
tomographic reconstruction. Unlike iterative algorithms which require a
distribution that is known a priori, deep reconstruction networks can learn a
prior distribution through sampling the training distributions. In this work,
we develop a Physics-assisted Generative Adversarial Network (PGAN), a two-step
algorithm for tomographic reconstruction. In contrast to previous efforts, our
PGAN utilizes maximum-likelihood estimates derived from the measurements to
regularize the reconstruction with both known physics and the learned prior.
Synthetic objects with spatial correlations are integrated circuits (IC) from a
proposed model CircuitFaker. Compared with maximum-likelihood estimation, PGAN
can reduce the photon requirement with limited projection angles to achieve a
given error rate. We further attribute the improvement to the learned prior by
reconstructing objects created without spatial correlations. The advantages of
using a prior from deep learning in X-ray tomography may further enable
low-photon nanoscale imaging.Comment: arXiv admin note: text overlap with arXiv:2111.0801
Quantum Hall effect and Landau level crossing of Dirac fermions in trilayer graphene
We investigate electronic transport in high mobility (\textgreater 100,000
cm/Vs) trilayer graphene devices on hexagonal boron nitride, which
enables the observation of Shubnikov-de Haas oscillations and an unconventional
quantum Hall effect. The massless and massive characters of the TLG subbands
lead to a set of Landau level crossings, whose magnetic field and filling
factor coordinates enable the direct determination of the
Slonczewski-Weiss-McClure (SWMcC) parameters used to describe the peculiar
electronic structure of trilayer graphene. Moreover, at high magnetic fields,
the degenerate crossing points split into manifolds indicating the existence of
broken-symmetry quantum Hall states.Comment: Supplementary Information at
http://jarilloherrero.mit.edu/wp-content/uploads/2011/04/Supplementary_Taychatanapat.pd
Killing Vector Fields in Three Dimensions: A Method to Solve Massive Gravity Field Equations
Killing vector fields in three dimensions play important role in the
construction of the related spacetime geometry. In this work we show that when
a three dimensional geometry admits a Killing vector field then the Ricci
tensor of the geometry is determined in terms of the Killing vector field and
its scalars. In this way we can generate all products and covariant derivatives
at any order of the ricci tensor. Using this property we give ways of solving
the field equations of Topologically Massive Gravity (TMG) and New Massive
Gravity (NMG) introduced recently. In particular when the scalars of the
Killing vector field (timelike, spacelike and null cases) are constants then
all three dimensional symmetric tensors of the geometry, the ricci and einstein
tensors, their covariant derivatives at all orders, their products of all
orders are completely determined by the Killing vector field and the metric.
Hence the corresponding three dimensional metrics are strong candidates of
solving all higher derivative gravitational field equations in three
dimensions.Comment: 25 pages, some changes made and some references added, to be
published in Classical and Quantum Gravit
Sublingual Immunization with M2-Based Vaccine Induces Broad Protective Immunity against Influenza
The ectodomain of matrix protein 2 (M2e) of influenza A virus is a rationale target antigen candidate for the development of a universal vaccine against influenza as M2e undergoes little sequence variation amongst human influenza A strains. Vaccine-induced M2e-specific antibodies (Abs) have been shown to display significant cross-protective activity in animal models. M2e-based vaccine constructs have been shown to be more protective when administered by the intranasal (i.n.) route than after parenteral injection. However, i.n. administration of vaccines poses rare but serious safety issues associated with retrograde passage of inhaled antigens and adjuvants through the olfactory epithelium. In this study, we examined whether the sublingual (s.l.) route could serve as a safe and effective alternative mucosal delivery route for administering a prototype M2e-based vaccine. The mechanism whereby s.l. immunization with M2e vaccine candidate induces broad protection against infection with different influenza virus subtypes was explored.A recombinant M2 protein with three tandem copies of the M2e (3M2eC) was expressed in Escherichia coli. Parenteral immunizations of mice with 3M2eC induced high levels of M2e-specific serum Abs but failed to provide complete protection against lethal challenge with influenza virus. In contrast, s.l. immunization with 3M2eC was superior for inducing protection in mice. In the latter animals, protection was associated with specific Ab responses in the lungs.The results demonstrate that s.l. immunization with 3M2eC vaccine induced airway mucosal immune responses along with broad cross-protective immunity to influenza. These findings may contribute to the understanding of the M2-based vaccine approach to control epidemic and pandemic influenza infections
GNPS - guided discovery of Madurastatin siderophores from the termite-associated actinomadura sp. RB99
In this study, we analyzed if Actinomadura sp. RB99
produces siderophores that that could be responsible for the
antimicrobial activity observed in co-cultivation studies.
Dereplication of high-resolution tandem mass spectrometry
(HRMS/MS) and global natural product social molecular
networking platform (GNPS) analysis of fungus-bacterium cocultures
resulted in the identification of five madurastatin
derivatives (A1, A2, E1, F, and G1), of which were four new
derivatives. Chemical structures were unambiguously confirmed
by HR-ESI-MS, 1D and 2D NMR experiments, as well as
MS/MS data and their absolute structures were elucidated
based on Marfey’s analysis, DP4+ probability calculation and
total synthesis. Structure analysis revealed that madurastatin
E1 (2) contained a rare 4-imidazolidinone cyclic moiety and
madurastatin A1 (5) was characterized as a Ga3+-complex.
The function of madurastatins as siderophores was evaluated
using the fungal pathogen Cryptococcus neoformans as model
organism. Based on homology models, we identified the
putative NRPS-based gene cluster region of the siderophores
in Actinomadura sp. RB99.The National Research Foundation of Korea, the Postdoctoral Research Program of Sungkyunkwan University, the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation). Open Access funding enabled and organized by Projekt DEAL.https://www.wiley-vch.de/en/shop/journals/234am2023BiochemistryGeneticsMicrobiology and Plant Patholog
Black tea extract prevents lipopolysaccharide-induced NF-κB signaling and attenuates dextran sulfate sodium-induced experimental colitis
<p>Abstract</p> <p>Background</p> <p>Black tea has been shown to elicit anti-oxidant, anti-carcinogenic, anti-inflammatory and anti-mutagenic properties. In this study, we investigated the impact of black tea extract (BTE) on lipopolysaccharide (LPS)-induced NF-κB signaling in bone marrow derived-macrophages (BMM) and determined the therapeutic efficacy of this extract on colon inflammation.</p> <p>Methods</p> <p>The effect of BTE on LPS-induced NF-κB signaling and pro-inflammatory gene expression was evaluated by RT-PCR, Western blotting, immunofluorescence and electrophoretic mobility shift assay (EMSA). The <it>in vivo </it>efficacy of BTE was assessed in mice with 3% dextran sulfate sodium (DSS)-induced colitis. The severity of colitis was measured by weight loss, colon length and histologic scores.</p> <p>Results</p> <p>LPS-induced IL-12p40, IL-23p19, IL-6 and IL-1β mRNA expressions were inhibited by BTE. LPS-induced IκBα phosphorylation/degradation and nuclear translocation of NF-κB/p65 were blocked by BTE. BTE treatment blocked LPS-induced DNA-binding activity of NF-κB. BTE-fed, DSS-exposed mice showed the less weight loss, longer colon length and lower histologic score compared to control diet-fed, DSS-exposed mice. DSS-induced IκBα phosphorylation/degradation and phosphorylation of NF-κB/p65 were blocked by BTE. An increase of cleaved caspase-3 and poly (ADP-ribose) polymerase (PARP) in DSS-exposed mice was blocked by BTE.</p> <p>Conclusions</p> <p>These results indicate that BTE attenuates colon inflammation through the blockage of NF-κB signaling and apoptosis in DSS-induced experimental colitis model.</p
Paraffin-enabled graphene transfer
The transfer process of as-grown graphene limits its electrical performance and reliability. Here, the authors develop a transfer approach using paraffin as a support layer and obtain wrinkle-reduced and clean large-area graphene retaining high mobility
The Flexibility of Nonconsciously Deployed Cognitive Processes: Evidence from Masked Congruence Priming
Background: It is well accepted in the subliminal priming literature that task-level properties modulate nonconscious processes. For example, in tasks with a limited number of targets, subliminal priming effects are limited to primes that are physically similar to the targets. In contrast, when a large number of targets are used, subliminal priming effects are observed for primes that share a semantic (but not necessarily physical) relationship with the target. Findings such as these have led researchers to conclude that task-level properties can direct nonconscious processes to be deployed exclusively over central (semantic) or peripheral (physically specified) representations. Principal Findings: We find distinct patterns of masked priming for "novel" and "repeated" primes within a single task context. Novel primes never appear as targets and thus are not seen consciously in the experiment. Repeated primes do appear as targets, thereby lending themselves to the establishment of peripheral stimulus-response mappings. If the source of the masked priming effect were exclusively central or peripheral, then both novel and repeated primes should yield similar patterns of priming. In contrast, we find that both novel and repeated primes produce robust, yet distinct, patterns of priming. Conclusions: Our findings indicate that nonconsciously elicited cognitive processes can be flexibly deployed over both central and peripheral representations within a single task context. While we agree that task-level properties can influence nonconscious processes, our findings sharply constrain the extent of this influence. Specifically, our findings are inconsistent with extant accounts which hold that the influence of task-level properties is strong enough to restrict the deployment of nonconsciously elicited cognitive processes to a single type of representation (i.e. central or peripheral).13 page(s
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